PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens

Rigoberto Ortiz-Hernández; Elmer Joel Millán-Casarrubias; Jeni Bolaños; Susana Munguía-Robledo; Carlos Vázquez-Calzada; Elisa Azuara-Licéaga; Jesús Valdés; Mario Alberto Rodríguez

doi:10.20944/preprints202411.0536.v1

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preprints.org > biology and life sciences > parasitology > doi: 10.20944/preprints202411.0536.v1 (registering DOI)

Preprint Article Version 1 This version is not peer-reviewed

PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens

Rigoberto Ortiz-Hernández

Elmer Joel Millán-Casarrubias

Jeni Bolaños

Susana Munguía-Robledo

Carlos Vázquez-Calzada

Elisa Azuara-Licéaga

Jesús Valdés

Mario Alberto Rodríguez

Version 1 : Received: 6 November 2024 / Approved: 7 November 2024 / Online: 7 November 2024 (13:25:15 CET)

How to cite: Ortiz-Hernández, R.; Millán-Casarrubias, E. J.; Bolaños, J.; Munguía-Robledo, S.; Vázquez-Calzada, C.; Azuara-Licéaga, E.; Valdés, J.; Rodríguez, M. A. PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens. Preprints 2024, 2024110536. https://doi.org/10.20944/preprints202411.0536.v1 Ortiz-Hernández, R.; Millán-Casarrubias, E. J.; Bolaños, J.; Munguía-Robledo, S.; Vázquez-Calzada, C.; Azuara-Licéaga, E.; Valdés, J.; Rodríguez, M. A. PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens. Preprints 2024, 2024110536. https://doi.org/10.20944/preprints202411.0536.v1

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MDPI and ACS Style

Ortiz-Hernández, R.; Millán-Casarrubias, E. J.; Bolaños, J.; Munguía-Robledo, S.; Vázquez-Calzada, C.; Azuara-Licéaga, E.; Valdés, J.; Rodríguez, M. A. PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens. Preprints 2024, 2024110536. https://doi.org/10.20944/preprints202411.0536.v1

APA Style

Ortiz-Hernández, R., Millán-Casarrubias, E. J., Bolaños, J., Munguía-Robledo, S., Vázquez-Calzada, C., Azuara-Licéaga, E., Valdés, J., & Rodríguez, M. A. (2024). PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens. Preprints. https://doi.org/10.20944/preprints202411.0536.v1

Chicago/Turabian Style

Ortiz-Hernández, R., Jesús Valdés and Mario Alberto Rodríguez. 2024 "PRMT5 Inhibitor EPZ015666 Decreases Viability and Encystment of Entamoeba invadens" Preprints. https://doi.org/10.20944/preprints202411.0536.v1

Abstract

PRMT5 methyltranserase is an enzyme that produces monomethyl arginine (MMA) as well as symmetric dimethyl arginine (sDMA), posttranslational modifications that regulate several cellu-lar processes, including stage conversion in parasitic protozoa. Entamoeba histolytica, the etiologic agent of human amebiasis, has two stages in its life cycle, the trophozoite, which is the replicative form, and the cyst, corresponding to the infective phase. The study of the molecular mechanisms that regulate differentiation in this parasite has been overdue by the lack of efficient protocols for in vitro encystment. For this reason, Entamoeba invadens, a reptile parasite, has been used as a dif-ferentiation model system for the genus. Here, we demonstrated the presence of sDMA in E. in-vadens, which increases during encystment, and identified the PRMT5 protein of this microorgan-ism (EiPRMT5). In addition, we performed 3D modeling of this enzyme, as well as its molecular docking with the PRMT5 inhibitor EPZ015666, which predicted the affinity of the drug for the ac-tive site of the enzyme. In agreement, EPZ015666 reduced trophozoite viability and encystment. Therefore, EiPRMT5 is a potential target for the inhibition of amebiasis spread.

Keywords

Entamoeba invadens; encystment; Symmetric arginine dimethylation; PRMT5

Subject

Biology and Life Sciences, Parasitology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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