Background and objective. Hip muscles lengthening is commonly associated with the normal function of the lumbar spine and lower extremities. Some evidence correlates hamstrings and iliopsoas tightness with low back pain (LBP). Undergraduates are more prone to LBP as they are involved in prolonged sitting and mal-posture. This study aims to assess the impact of hip muscles lengthening on LBP. Methods: A descriptive study of 70 students who were recruited from Zefat Academic College. Measurement of hamstrings and iliopsoas muscles lengthening as well as a constructive questionnaire were used. Results: The majority of participants (80% for the Hamstrings and 96% for the Iliopsoas) manifested normal muscle lengthening. Muscle flexibility was significantly higher among females. The logistic regression analyses revealed that hamstrings lengthening (right), and stress-related study, are significantly associated with LBP. Conclusions: The current study indicates that muscle length is female-dependent and right-left muscle length is symmetrical. Increased hamstrings length could be related to LBP.