This study addresses the pressing need for standardized methodologies to quantify microplastics within the fine fraction of municipal solid waste (MSW), often overlooked despite its potential en-vironmental impact. Five extraction protocols were evaluated to identify the most effective method for isolating microplastics in fine waste. These were specifically applied to samples from the Uni-versidad Autónoma Metropolitana and one transfer station in Mexico City. A potassium hydroxide digestion protocol with subsequent flotation and centrifugation steps achieved optimal results, ensuring complete organic matter degradation and high microplastic recovery. Subsequent analyses revealed notable concentrations of microplastics, primarily fragments and fibers, with higher abundance at the university site. Statistical tests confirmed significant differences between sample sites. These findings highlight the vulnerability of MSW fine fractions to microplastic contamination and underline the importance of targeted waste management strategies. This research contributes to understanding microplastic behavior in waste management systems and emphasizes the need for mitigation efforts to prevent environmental contamination.