Pomegranate cultivation in the Indian peninsular region, particularly in Maharashtra, significantly contributes to farmers' economic well-being but faces threats from microbial diseases, notably bacterial blight caused by Xanthomonas axonopodis pv. punicae. This study aimed to evaluate the antibacterial potential of bacteriocin produced by Lactobacillus helveticus PMP-6 (Genebank accession number MT 444488) against said pathogen. The bacteriocin was purified, resulting in a specific activity increase from 233.849 AU/mg to 3615.81 AU/mg. Antibacterial assays demonstrated that the purified bacteriocin effectively inhibited X. axonopodis Xpp-1 at 40°C and pH 7.0, with a minimal inhibitory concentration (MIC) of 300 mg/ml and a minimum bactericidal concentration (MBC) of 400 mg/ml, particularly in the absence of proteases. SDS-PAGE analysis revealed a molecular weight of approximately 17 kDa for the isolated and purified bacteriocin. The efficacy was validated through the Detached Leaf Assay (DLA), confirming that treated samples exhibited no symptom development over 96 hours. This research represents the first documentation of the antagonistic activity of Lactobacillus helveticus PMP-6 against X. axonopodis, suggesting its potential as an eco-friendly biocontrol agent to enhance Pomegranate production and promote sustainable agriculture while reducing reliance on agrochemicals.