Pancreatitis is a prominent and severe type of inflammatory disorder with has grabbed a lot of scientific and clinical interest to prevent its onset. It should be detected early to avoid the development of serious complications, which occur due to long term damage. The accurate measurement of biomarkers that are released from the pancreas during inflammation is essential for the detection and early treatment of patients with severe acute and chronic pancreatitis, and this is sub-optimally performed in clinically relevant practices mainly due to the complexity of the procedure and the cost of the treatment. Clinically available tests for early detection of pancreatitis are often time consuming. Early detection of pancreatitis also relates to disorders of the exocrine pancreas, such as cystic fibrosis in the hereditary form and the cystic fibrosis-like syndrome in the acquired form of pancreatitis which are genetic disorders whose symptoms can be correlated to the presence of over expression of specific markers such as creatinine in biological fluids like urine. In this review we have studied how to develop a minimally invasive system using hydrogel-based biosensor, which are highly absorbent and biocompatible polymers which can respond to specific stimuli such as enzymes, pH, temperature, or the presence of biomarkers. These biosensors are helpful for real-time health monitoring, environmental monitoring, and medical diagnostics since they translate biological reactions into quantifiable data. These analytical devices can be used to improve early detection of severe pancreatitis in real time.