Antimicrobial resistance (AMR) remains a persistent world-wide health concern. Poultry production in Nigeria has become a large-scale venture and is largely characterised by intensive management system. This intensive management system disposes to more antibiotic use and thus a greater risk of AMR. Previous reviews on AMR data in Nigeria have not considered the accuracy of methods and interpretation criteria. Therefore, in contribution to the “One Health” AMR Surveillance system, which is one of the five focus areas described by the Nigeria action plan on AMR control, this comprehensive review collates, curate, and analyse AMR published data in the Nigeria poultry sector and assessed the AST methods and interpretation criteria. A search of AMR reports from poultry in Nigeria prior to 2020 after screening for relevance yielded 69 studies from 2005-2020 with 91 AMR reports (some studies reported more than one organism). From these studies we reviewed the organisms reported, antibiotics tested, resistance to tested antibiotics, distribution of reports within the country’s geopolitical zones, multidrug resistance pattern, the inclusion or absence of control strains in antimicrobial testing, the antimicrobial testing method and interpretative criteria used.There was no AMR study in poultry earlier than 2005 in Nigeria, highest number of study was in 2019 (n=12). South-Western region has the highest number (n = 27) of study and the North-Western region has the lowest (n = 5). Organisms mostly reported were Salmonella, 31%, Escherichia coli, 26% and Staphylococcus aureus, 15%). Other organisms (≤5%) were Campylobacter, Klebsiella, Bacillus, Listeria, Enterobacter, Proteus, Norcardia, Streptococcus, Alkaligenes, Shigella, Micrococcus, Enterococcus, Ochrobacturm, and Yersinia. The antimicrobial susceptibility testing (AST) method mostly used was disc diffusion (80%), also majority (95.7%) of the studies used the CLSI standards for interpretation and 79.7% of the studies did not mention the use of control strains for AST. The 69 studies reviewed tested 69 antimicrobials belonging to 17 classes of Antibiotics; the most tested classes of antimicrobial were β-lactams (33%), quinolones (21%) and aminoglycosides (15.4%). All of the organisms showed varying multi-drug resistance; greater proportion (50-100%) has MAR Index greater than 0.2. The review of the various studies showed very high multi-drug resistance among the various organisms of poultry origin and also a gap in AST carried out in Nigeria. We therefore recommend that AST research methods must be of global standard and additional efforts should be made towards education of the general public on best practices of antibiotics usage and the dangers of AMR.