In Latvia, livestock depredation by wolves has increased during the last two decades. Most attacks had occurred in summer and autumn within wolf hunting season. Cumulative numbers of wolf attacks and number of affected sheep per year at regional forest management units were analyzed in relation to estimated wolf density, extent of culling and proportion of juveniles, as well as sheep density and estimated number of wild prey. The response variables (cumulative number of attacks and cumulative number of affected sheep) were modelled by a negative binomial regression, testing effects of every covariate separately and building models from the significant covariates. Depredation level was related to sheep density and estimated wolf population size. No reducing effect was found for culling, and even greater depredation rate was expected at higher proportions of culled wolves. Estimated number of wild prey or proportion of juvenile wolves had an insignificant effect. However, greater numbers of affected sheep were expected at higher red deer density, suggesting increased opportunistic livestock depredation when the red deer may locally outcompete the preferred wolf prey – roe deer.