This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. The article examines various analytical tech-niques, including high-performance liquid chromatography (HPLC), liquid chromatog-raphy-mass spectrometry (LC-MS), and enzyme-linked immunosorbent assay (ELISA), while highlighting their respective advantages and limitations. Additionally, the review delves into recent advancements in sample preparation, extraction, and quantification methods, offering valuable insights into the accurate and efficient determination of glucosinolate content across diverse plant materials. Furthermore, it underscores the critical importance of standardization and validation of these methodologies to ensure reliable glucosinolate analysis in both scientific research and industrial applications.