Loess is a special soil with high water sensitivity, which covers a large area in northwest China. Cracks are prone to generate in loess under the arid and semi-arid climate, which will provide the preferential channel for water and reduce the mechanical properties of soils. It is of great significance to understand the evolution characteristics and mechanisms of the cracks in loess areas. At present, the researches on cracks in soils are mainly concentrated in the characteristics of different cracking patterns. However, the spacing cracks are mostly discussed in materials like rock and concrete, rather than soils. The cracking characteristics and mechanisms of spacing cracks in loess is still inadequate. In this research, the drying tests of loess are carried out with different sizes of specimens. The parameters of spacing cracks and local strain distributions of the loess samples are obtained via PCAS and DIC methods during desiccation, respectively. The cracking modes, space cracking laws like insertion and saturation of spacing cracks are revealed. Finally, the size effect on the characteristics of spacing cracks is verified with a discrete element software MatDEM.