Camphora longepaniculata is an important economic crop renowned for its rich volatile terpene compounds. Terpene synthases (TPS) are key enzymes in the biosynthesis of these compounds, playing significant roles in plant growth, development, and secondary metabolism. In this study, a total of 86 TPS genes were identified in Camphora longepaniculata, which were classified into five groups based on their evolutionary relationships. Analysis of cis-regulatory elements revealed associations between TPS genes and processes related to plant growth, development, and environmental stress responses. Gene Ontology (GO) enrichment analysis indicated that these TPS genes are predominantly linked to various enzymatic activities. Furthermore, analysis of duplication events revealed that tandem duplications (TD) and whole genome duplications (WGD) are major driving forces in the evolution of the TPS gene family. Notably, 18 TPS genes were found to be upregulated in high essential oil content varieties of Camphora longepaniculata. These findings lay a solid foundation for further exploration of the functions of TPS genes in Camphora longepaniculata.

Background: On 1 January 2024, the Noto Peninsula was struck by a 7.6 magnitude earthquake. In the previous article, The food provided in the evacuee shelters proved insufficient in energy and protein for the older adults, 65% of whom visited our emergency clinic. Methods: (1) analyze the amino acid and fatty acid composition of 11 evacuation meals using the "Duplicated Combination" Model, (2) assess the adequacy of the amino acid and fatty acid composition, the digestible essential amino acid score (DIAAS) was calculated and analyzed in relation to the half-life determined by the N-terminal amino acid of the proteins.. Linoleic acid (LA) and alpha-linolenic acid (ALA) levels were compared with EFSA recommendations, (3) The national survey of emergency food stocks in hospitals and social care institutions conducted in Jan, 2024 was analyzed. Results: (1) DIAAS was less than 1.00 for all 11 foods provided and was considered inadequate, (2) the half-life of the protein, whose N-terminal valine has a half-life of 100 hours, must be considered a possible deficiency when living in a shelter for more than a week, (3) LA and ALA levels were less than 40% of EFSA recommended, (4) the nationwide survey found that 80% of people have a 3-day supply and are likely to be energy and protein deficient. Conclusion: Analysis of foods in evacuation shelters after the Noto Peninsula earthquake revealed that the quality of amino acids and fatty acids seem inadequate according to DIAAS and EFSA recommendations. The “Duplicated Combination” Model might be useful to investigate the quality of foods in the shelters.