Hypothesis
Version 3
Preserved in Portico This version is not peer-reviewed
Encapsulated Bacteria with a Light-Repressed Deadman Switch for Liver Gene Delivery
Version 1
: Received: 24 April 2024 / Approved: 24 April 2024 / Online: 25 April 2024 (09:00:58 CEST)
Version 2 : Received: 26 April 2024 / Approved: 26 April 2024 / Online: 26 April 2024 (14:49:58 CEST)
Version 3 : Received: 9 May 2024 / Approved: 10 May 2024 / Online: 10 May 2024 (11:49:27 CEST)
Version 4 : Received: 10 May 2024 / Approved: 13 May 2024 / Online: 13 May 2024 (14:33:20 CEST)
Version 2 : Received: 26 April 2024 / Approved: 26 April 2024 / Online: 26 April 2024 (14:49:58 CEST)
Version 3 : Received: 9 May 2024 / Approved: 10 May 2024 / Online: 10 May 2024 (11:49:27 CEST)
Version 4 : Received: 10 May 2024 / Approved: 13 May 2024 / Online: 13 May 2024 (14:33:20 CEST)
How to cite: Renteln, M. Encapsulated Bacteria with a Light-Repressed Deadman Switch for Liver Gene Delivery. Preprints 2024, 2024041631. https://doi.org/10.20944/preprints202404.1631.v3 Renteln, M. Encapsulated Bacteria with a Light-Repressed Deadman Switch for Liver Gene Delivery. Preprints 2024, 2024041631. https://doi.org/10.20944/preprints202404.1631.v3
Abstract
Adeno-associated viruses (AAVs) have been used for liver gene therapy. Hemgenix and Roctavian are AAV-based treatments for hemophilia B and A, respectively. They cost $3 million and $2.8 million per dose, respectively. While AAV vectors may eventually be cheaper to mass produce, a bacteria-based DNA delivery system might be much cheaper for patients. Also, “bactofection” would allow for the delivery of much larger DNA packages. Such a bacterial system may now be possible, and a prototype for the liver could possibly be developed immediately.
Keywords
bacterial capsule; caged luciferin/luciferase; CY3PA; light-activated allosteric protein switch; Deadman switch; bactofection
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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