Version 1
: Received: 12 April 2018 / Approved: 12 April 2018 / Online: 12 April 2018 (16:18:09 CEST)
How to cite:
Agbozo, E. Y.; Dumashie, E.; Boakye, D. A.; de Souza, D. K. Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability. Preprints2018, 2018040170. https://doi.org/10.20944/preprints201804.0170.v1
Agbozo, E. Y.; Dumashie, E.; Boakye, D. A.; de Souza, D. K. Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability. Preprints 2018, 2018040170. https://doi.org/10.20944/preprints201804.0170.v1
Agbozo, E. Y.; Dumashie, E.; Boakye, D. A.; de Souza, D. K. Effects of Lyophilization and Storage Temperature on Wuchereria bancrofti Antigen Sensitivity and Stability. Preprints2018, 2018040170. https://doi.org/10.20944/preprints201804.0170.v1
APA Style
Agbozo, E. Y., Dumashie, E., Boakye, D. A., & de Souza, D. K. (2018). Effects of Lyophilization and Storage Temperature on <em>Wuchereria bancrofti</em> Antigen Sensitivity and Stability. Preprints. https://doi.org/10.20944/preprints201804.0170.v1
Chicago/Turabian Style
Agbozo, E. Y., Daniel A. Boakye and Dziedzom K. de Souza. 2018 "Effects of Lyophilization and Storage Temperature on <em>Wuchereria bancrofti</em> Antigen Sensitivity and Stability" Preprints. https://doi.org/10.20944/preprints201804.0170.v1
Abstract
Antigen-based rapid diagnostic tests for Lymphatic filariasis do not come with quality control (QC) materials, and research and disease control programmes rely on stored positive plasma samples. This study was undertaken to evaluate the use of freeze-dried Wuchereria bancrofti antigen positive plasma samples to serve as QC materials for LF RDTs. 10 well characterized W. bancrofti positive samples were lyophilized and stored at 4°C, 28°C and 40°C. The samples were evaluated using the Filaria Test Strips before lyophilization and after one and three (3) months of storage. The sensitivity and stability of the lyophilized samples were evaluated. The results revealed a loss of sensitivity and stability with increasing temperature and duration of storage. The results are further discussed in terms of the use of Dried Blood Spot (DBS) in diagnostics studies on LF requiring quantitative assessments, and the need for thoughtful DBS preparation and storage.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.