Article
Version 1
Preserved in Portico This version is not peer-reviewed
Inonotus Obliquus extracts as an inhibitor of α-MSH-induced melanogenesis in B16F10 mouse melanoma cells
Version 1
: Received: 8 November 2018 / Approved: 9 November 2018 / Online: 9 November 2018 (13:36:36 CET)
A peer-reviewed article of this Preprint also exists.
Lee, E.J.; Cha, H.J. Inonotus obliquus Extract as An Inhibitor of α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells. Cosmetics 2019, 6, 9. Lee, E.J.; Cha, H.J. Inonotus obliquus Extract as An Inhibitor of α-MSH-Induced Melanogenesis in B16F10 Mouse Melanoma Cells. Cosmetics 2019, 6, 9.
Abstract
Melanogenesis is a biosynthetic pathway for producing of the pigment melanin in human skin. Tyrosinase, a key enzyme, catalyzing is the first step in melanogenesis and the downregulation of the tyrosinase enzyme activity is the most reported method for anti-melanogenesis. According to the hyperpigmentation as an important issue in cosmetic industry, there is a big demand for melanogenesis inhibitors. In the present study, we identified the anti-melanogenic effect of Inonotus Obliquus in α-MSH-induced B16F10 mouse melanoma cells as a new inhibitor. Comparing with control and Inonotus Obliquus extracts treated B16F10, we identified melanin contents, tyrosinase activity, tyrosinase mRNA and protein expression, MITF activity using a constructed plasmid. As shown in these results, we demonstrated that Inonotus Obliquus extracts down-regulated melanin synthesis using down-regulating activity and expression of tyrosinase which is key enzyme to produce melanin. In addition, we revealed expression of tyrosinase is regulated by MITF through repressing MITF transcriptional activity. Inonotus Obliquus extracts has potential to repress melanogenesis and decreased of hyperpigmentation and to use as cosmetic ingredient.
Keywords
Inonotus Obliquus, Melanogenesis, B16F10, Tyrosinase, MITF
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Comments (0)
We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.
Leave a public commentSend a private comment to the author(s)
* All users must log in before leaving a comment