Version 1
: Received: 11 December 2019 / Approved: 12 December 2019 / Online: 12 December 2019 (05:07:02 CET)
Version 2
: Received: 15 January 2020 / Approved: 16 January 2020 / Online: 16 January 2020 (05:13:40 CET)
Version 3
: Received: 14 April 2020 / Approved: 15 April 2020 / Online: 15 April 2020 (07:59:18 CEST)
How to cite:
Lainetti, P. D. F.; Brandi, A.; Filho, A. F. L.; Prado, M. C. M.; Kobayashi, P. E.; Laufer-Amorim, R.; Fonseca-Alves, C. E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints2019, 2019120162
Lainetti, P. D. F.; Brandi, A.; Filho, A. F. L.; Prado, M. C. M.; Kobayashi, P. E.; Laufer-Amorim, R.; Fonseca-Alves, C. E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints 2019, 2019120162
Lainetti, P. D. F.; Brandi, A.; Filho, A. F. L.; Prado, M. C. M.; Kobayashi, P. E.; Laufer-Amorim, R.; Fonseca-Alves, C. E. Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability in vitro and in vivo. Preprints2019, 2019120162
APA Style
Lainetti, P. D. F., Brandi, A., Filho, A. F. L., Prado, M. C. M., Kobayashi, P. E., Laufer-Amorim, R., & Fonseca-Alves, C. E. (2020). Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability <em>in vitro</em> and<em> in vivo</em>. Preprints. https://doi.org/
Chicago/Turabian Style
Lainetti, P. D. F., Renée Laufer-Amorim and Carlos Eduardo Fonseca-Alves. 2020 "Establishment and Characterization of Canine Mammary Gland Carcinoma Cell Lines with Vasculogenic Mimicry Ability <em>in vitro</em> and<em> in vivo</em>" Preprints. https://doi.org/
Abstract
Mammary neoplasms affect a population of intact and elderly female dogs and 50% of these neoplasms are malignant. In order to study this disease, cell culture is as a promising preclinical model, creating the opportunity to deposit cell lines at a cell bank, allowing a great reproducibility of the assays and making the validation of the results more reliable. Another important aspect is the possibility to establish models for the better understanding of tumour characteristics, such as vasculogenic mimicry. VM is related to cancer cells able to generate vascular-like structures from epithelial cells, mimicking the vasculogenic process and it is closely related to patient’s prognosis. Due the importance of cancer cell lines in preclinical models, this study aimed to establish and characterize ten cell lines from canine mammary gland tumours according to immunophenotype and tumorigenicity, and with its ability to form vasculogenic mimicry-like structures in vitro and in vivo. Cell cultures were evaluated for morphology, phenotype, vasculogenic mimicry and tumorigenicity abilities. All cell lines presented spindle shape morphology and expressed concomitant pan-cytokeratin and cytokeratin 8/18. Four cell lines had vasculogenic mimicry ability in vitro and two of them showed in vivo tumorigenic potential and forming VM in the xenotransplant tumour. Cell characterization of those lines will help to create a database for more knowledge of mammary carcinomas in dogs, including studies of tumor behavior and new therapeutic targets.
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
23 January 2020
Commenter:
Amos Bairoch
The commenter has declared there is no conflict of interests.
Comment:
Nice article, but 2 things need to be done before it is published:
1) Provide the information on the breed and age at sampling of the dogs used as donors for these cell lines.
2) Change the names of the cell lines: using CMn or MMn is a very bad idea: there are already many other cell lines with such short conflicting names. You could prefix these designations with the acronym of your institution, for example: UNESP-CM1. This is what I will use to create preliminary entries in the Cellosaurus for your cell lines.
The commenter has declared there is no conflict of interests.
Comment:
Dear Dr. Bairoch
Thank you so much for your kind comment. Both suggestions are great!
We are organizing the new version of our manuscript and we will include both alterations.
Once again, thank you for you comments.
We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.
Commenter: Patricia Lainetti
Commenter's Conflict of Interests: Author
Commenter: Amos Bairoch
The commenter has declared there is no conflict of interests.
1) Provide the information on the breed and age at sampling of the dogs used as donors for these cell lines.
2) Change the names of the cell lines: using CMn or MMn is a very bad idea: there are already many other cell lines with such short conflicting names. You could prefix these designations with the acronym of your institution, for example: UNESP-CM1. This is what I will use to create preliminary entries in the Cellosaurus for your cell lines.
Commenter:
The commenter has declared there is no conflict of interests.
Thank you so much for your kind comment. Both suggestions are great!
We are organizing the new version of our manuscript and we will include both alterations.
Once again, thank you for you comments.