Version 1
: Received: 5 January 2020 / Approved: 9 January 2020 / Online: 9 January 2020 (04:44:49 CET)
How to cite:
Goudarzi, S. H.; Kabat, B.; Cannon, M. L.; Gashkoff, M.; Zurek, R. Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols. Preprints2020, 2020010068. https://doi.org/10.20944/preprints202001.0068.v1
Goudarzi, S. H.; Kabat, B.; Cannon, M. L.; Gashkoff, M.; Zurek, R. Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols. Preprints 2020, 2020010068. https://doi.org/10.20944/preprints202001.0068.v1
Goudarzi, S. H.; Kabat, B.; Cannon, M. L.; Gashkoff, M.; Zurek, R. Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols. Preprints2020, 2020010068. https://doi.org/10.20944/preprints202001.0068.v1
APA Style
Goudarzi, S. H., Kabat, B., Cannon, M. L., Gashkoff, M., & Zurek, R. (2020). Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols. Preprints. https://doi.org/10.20944/preprints202001.0068.v1
Chicago/Turabian Style
Goudarzi, S. H., Maggie Gashkoff and Rachel Zurek. 2020 "Pilot Study of the SCFA Headspace Analysis of Streptococcus mutans Metabolites in Media with and without Polyols" Preprints. https://doi.org/10.20944/preprints202001.0068.v1
Abstract
This pilot study of Streptococcus mutans ATCC 35668 grown in media with and without polyols (erythritol) measured the resultant metabolites, including the short chain fatty acids by using head space analysis. Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing no polyols or either erythritol or xylitol and Streptococcus mutans (ATCC 35668) was grown aerobically. After 48 hours of growth the supernatant were harvested and centrifuged to pellet bacteria. Supernatants were removed from bacterial pellets then submitted for Short Chain Fatty Acid (SCFA) analysis with an Agilent Technologies (Santa Clara, CA 95051) system configured from three components, a 5973 mass selective detector, a 6890N gas chromatographer, and a 7697A headspace sampler. Streptococcus mutans growing in Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose but containing no polyols produced the following short chain fatty acids: methyl isovalerate, acetic acid, propionic acid, butanoic acid, pentanoic acid, ethyl butaric acid, 4-methylvaleric acid, hexanoic acid. When the Brain Heart Infusion Broth (BHI2 or BHI10) supplemented with 2% or 10% sucrose containing erythritol was used as media for this Streptococcus mutans strain, the following were produced: ethanol, acetoin, and acetic acid. Our results would suggest that constituents of the media may affect the bacterial metabolite production.
Keywords
prebiotics; polyols; short chain fatty acids, Headspace Analysis
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.