Euphorbia helioscopia is a common weed of the agricultural crops, it not only competes with the crop plants for food and space but also causes a significant losses to the final crop production. Therefore, it was need of the hour to find some sustainable approach to control this weed in agricultural lands. An experiment was conducted to access the potential of various fungal organisms (Alternaria tenuissimia, Alternaria alternata and Fusarium oxysporum) to inhibit the growth of Euphorbia helioscopiai in vegetable crops. E. helioscopia was transplanted in March 2020, the experiment was designed with three treatments and having five replicates for each treatment. Plantlets were inoculated with different concentrations (1×10³, 1×10⁵, 1×10⁷ spores/ml.) of fungal spore suspensions of selected fungi. A control experiment was also added in the experiment which was not inoculated with any of the spore suspension. Maximum disease severity was observed in case of inoculation with the spore suspension of Alternaria tenuissimia. The agronomic traits which were observed after inoculation as indicator were number of infected leaves, number of infected plants, and number of spots on leaves. Control experiment has exhibited the plants without any sign of infection.