Version 1
: Received: 21 January 2021 / Approved: 25 January 2021 / Online: 25 January 2021 (13:03:02 CET)
How to cite:
Jimenez Jr, V. M.; Ostrom, E. L.; Traustadόttir, T.; Monroy, F. P. Sulforaphane Pre-treatment Protects From Alcohol-induced Phagocytic Dysfunction During Burkholderia thailandensis E264 Infection. Preprints2021, 2021010495. https://doi.org/10.20944/preprints202101.0495.v1
Jimenez Jr, V. M.; Ostrom, E. L.; Traustadόttir, T.; Monroy, F. P. Sulforaphane Pre-treatment Protects From Alcohol-induced Phagocytic Dysfunction During Burkholderia thailandensis E264 Infection. Preprints 2021, 2021010495. https://doi.org/10.20944/preprints202101.0495.v1
Jimenez Jr, V. M.; Ostrom, E. L.; Traustadόttir, T.; Monroy, F. P. Sulforaphane Pre-treatment Protects From Alcohol-induced Phagocytic Dysfunction During Burkholderia thailandensis E264 Infection. Preprints2021, 2021010495. https://doi.org/10.20944/preprints202101.0495.v1
APA Style
Jimenez Jr, V. M., Ostrom, E. L., Traustadόttir, T., & Monroy, F. P. (2021). Sulforaphane Pre-treatment Protects From Alcohol-induced Phagocytic Dysfunction During Burkholderia thailandensis E264 Infection. Preprints. https://doi.org/10.20944/preprints202101.0495.v1
Chicago/Turabian Style
Jimenez Jr, V. M., Tinna Traustadόttir and Fernando P Monroy. 2021 "Sulforaphane Pre-treatment Protects From Alcohol-induced Phagocytic Dysfunction During Burkholderia thailandensis E264 Infection" Preprints. https://doi.org/10.20944/preprints202101.0495.v1
Abstract
Binge drinking is associated with increased mortality and morbidity. Burkholderia pseudomallei, the causative agent of pneumonic melioidosis can occur in healthy humans; however, binge alcohol intoxication is a major risk factor. Previous findings indicate that a single binge alcohol episode increases Burkholderia spp. infection by reducing alveolar macrophage function. The aim of this study was to test the ability of the phytonutrient sulforaphane (SFN) to rescue the phagocytic function of alveolar macrophages when infected with Burkholderia spp. in vitro. B. thailandensis E264 was used as a useful BSL-1 model to determine the effects of SFN pre-treatment. The primary outcome was macrophage phagocytic killing, while the secondary outcome was the nuclear factor (erythroid-derived 2)-like (Nrf2) signaling response measured by western blot analysis. Results indicate that alveolar macrophages pre-treated with SFN (5 M) and challenged with 0.2% (v/v) alcohol for 3 or 8 h prior to live B. thailandensis infection improved intracellular killing of B. thailandensis ~2-fold compared to MH-S cells treated with alcohol alone. These data demonstrate that SFN may be an effective pre-treatment option to prevent alcohol mediated immune dysfunction and restore macrophage phagocytic killing of Burkholderia spp. and other similar opportunistic pathogens.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.