The construction industries face several challenges related to productivity. Productivity mainly depends upon labour effort and performance. The poor productivity of labour is one of the major reasons of time over-runs and increasing cost in construction projects. Our study aims to build a comprehensive assessment on the relationship between various factors that affect labour productivity in the construction of prestressed concrete Buildings in Ghana. A sample of 200 re-spondents collected from workers of Construction Company in Ghana. The questionnaire was designed which comprises two parts; the first part contains background information of the staff of construction company while second section explores the opinion of the staff regarding factors af-fecting labour productivity. The Smart-PLS was utilized to analyze and estimate the relationship among construct variables. By utilizing relative importance index and multiple linear regressions, it is identified that the management factor such as inadequate incentives; material factor such as poor quality of material; labour factors such as poor quality and training of labors; supervision factor such as incompetence of site supervisor; equipment factor such as frequent damage of equipment have a negative and significant relationship with labour productivity in the construc-tion of prestressed concrete buildings in Ghana. The findings of this study recommend that in order to improve labour productivity, the construction industries must conduct labour productivity measurements by adopting schedule for procurement of materials, safety programs, motivational system, and frequent meeting with project professionals.