Version 1
: Received: 17 June 2021 / Approved: 18 June 2021 / Online: 18 June 2021 (15:26:13 CEST)
How to cite:
Pfeiffer, P.; Coates, J. R.; Kennedy, A.; Getchell, K.; Kosa, E.; Agbas, A. Exosome TDP-43 Profile in Canine Model of ALS: A Preliminary Study in Developing Surrogate Biomarker. Preprints2021, 2021060488. https://doi.org/10.20944/preprints202106.0488.v1
Pfeiffer, P.; Coates, J. R.; Kennedy, A.; Getchell, K.; Kosa, E.; Agbas, A. Exosome TDP-43 Profile in Canine Model of ALS: A Preliminary Study in Developing Surrogate Biomarker. Preprints 2021, 2021060488. https://doi.org/10.20944/preprints202106.0488.v1
Pfeiffer, P.; Coates, J. R.; Kennedy, A.; Getchell, K.; Kosa, E.; Agbas, A. Exosome TDP-43 Profile in Canine Model of ALS: A Preliminary Study in Developing Surrogate Biomarker. Preprints2021, 2021060488. https://doi.org/10.20944/preprints202106.0488.v1
APA Style
Pfeiffer, P., Coates, J. R., Kennedy, A., Getchell, K., Kosa, E., & Agbas, A. (2021). Exosome TDP-43 Profile in Canine Model of ALS: A Preliminary Study in Developing Surrogate Biomarker. Preprints. https://doi.org/10.20944/preprints202106.0488.v1
Chicago/Turabian Style
Pfeiffer, P., Edina Kosa and Abdulbaki Agbas. 2021 "Exosome TDP-43 Profile in Canine Model of ALS: A Preliminary Study in Developing Surrogate Biomarker" Preprints. https://doi.org/10.20944/preprints202106.0488.v1
Abstract
Blood-based biomarkers are much-needed diagnostic and prognostic tools for ALS. Canine degenerative myelopathy (DM) is recognized animal disease model to study the biology of human ALS. Serum derived exosomes are potential carrier that transport intercellular hormone-like messengers, together with their stability as carrier of proteins and RNA, make them ideal as biomarkers for a variety of diseases and biological processes. We study exosomal TDP-43 pattern as a surrogate biomarker that reflects biochemical changes in central nervous system. We isolated exosomes from canine serum using commercial exosome isolation reagents. TDP-43 and SOD1 profile in spinal cord homogenate lysate and that of serum-derived exosomes were found elevated in dogs with DM. We conclude levels of spinal cord TDP-43 and serum-derived exomes were similar in TDP-43 profiling, which warrant further investigation of disease sensitivity and specificity for establishing as a blood-based biomarker in canine DM.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.