Article
Version 1
Preserved in Portico This version is not peer-reviewed
Flexible Multiplane Structured Illumination Microscope With a Four-Camera Detector
Version 1
: Received: 29 April 2022 / Approved: 4 May 2022 / Online: 4 May 2022 (12:24:22 CEST)
A peer-reviewed article of this Preprint also exists.
Johnson, K.A.; Noble, D.; Machado, R.; Paul, T.C.; Hagen, G.M. Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector. Photonics 2022, 9, 501. Johnson, K.A.; Noble, D.; Machado, R.; Paul, T.C.; Hagen, G.M. Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector. Photonics 2022, 9, 501.
Abstract
Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a simple multi-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This simple setup allows for the acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics.
Keywords
structured illumination; fluorescence; brain; multi-camera
Subject
Biology and Life Sciences, Biophysics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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