The demand for an efficient utilization of abundant biomasses is growing for the production of biogas and valuable bioproducts. Lignocellulose biomass is a cheap and most abundant carbon source for the production of biofuels such as bioethanol, biobutanediol, and other bio-based chemicals. Due to its complex heterogeneity, its hydrolysis gives rise to a mixture of sugars, mainly glucose; a hexose and xylose; a pentose. Glucose is the most abundant carbohydrate monomer. Most microorganisms have evolved the ability to utilize it preferably due to carbon catabolite repression regulatory mechanism at the detriment of the pentoses. Some microbes even lack the ability to utilize them. This has led to the sequential use of these sugars and accompanying reduced productivity due to inadequate utilization of the pentoses. Also, this sequential utilization of the sugars takes time and makes the overall processes economically costly. Since lignocellulose hydrolysates comprise both hexoses and pentoses, the catabolism of these sugar mixtures to biofuels will require an efficient microbial strain capable of simultaneous utilization. The use of CCR negative mutants can achieve this. CCR negative mutants simultaneously utilize pentoses and hexoses, ensuring an improved fermentation efficacy and greater productivity, thus, making the overall bioprocess economically feasible. This article reviewed several approaches employed in creating these mutant microorganisms. A brief insight on carbon catabolite repression and phosphostransferase system were made. It also highlighted the biogas production processes, factors affecting anaerobic digestion, lignocellulosic biomass structure, challenges with their use and solutions to overcoming the challenges.