Rammler, T.; Wackenhut, F.; Rapp, J.; zur Oven-Krockhaus, S.; Forchhammer, K.; Meixner, A.J.; Harter, K. Analysis of Fast Fluorescence Kinetics of a Single Cyanobacterium Trapped in an Optical Microcavity. Plants2023, 12, 607.
Rammler, T.; Wackenhut, F.; Rapp, J.; zur Oven-Krockhaus, S.; Forchhammer, K.; Meixner, A.J.; Harter, K. Analysis of Fast Fluorescence Kinetics of a Single Cyanobacterium Trapped in an Optical Microcavity. Plants 2023, 12, 607.
Rammler, T.; Wackenhut, F.; Rapp, J.; zur Oven-Krockhaus, S.; Forchhammer, K.; Meixner, A.J.; Harter, K. Analysis of Fast Fluorescence Kinetics of a Single Cyanobacterium Trapped in an Optical Microcavity. Plants2023, 12, 607.
Rammler, T.; Wackenhut, F.; Rapp, J.; zur Oven-Krockhaus, S.; Forchhammer, K.; Meixner, A.J.; Harter, K. Analysis of Fast Fluorescence Kinetics of a Single Cyanobacterium Trapped in an Optical Microcavity. Plants 2023, 12, 607.
Abstract
Photosynthesis is one the most important biological processes on earth, producing life-giving oxygen and is the basis for a large variety of plant products. Measurable properties of photosynthesis provide information about its biophysical state and, in turn, the physiological conditions of a photoautotrophic organism. For instance, chlorophyll fluorescence of an intact photosystem is not linear as in the case of a single fluorescent dye in solution, but shows temporal changes related to the quantum yield of the photosystem. Commercial photosystem analyzers already use the fluorescence kinetics characteristics of photosystems to infer the viability of organisms under investigation. Here, we provide a novel approach based on an optical Fabry-Pérot microcavity or that enables the readout of photosynthetic properties and activity for an individual cyanobacterium. This approach offers a completely new dimension of information, which would normally be lost due to averaging in ensemble measurements obtained from a large population of bacteria.
Keywords
cyanobacteria; photosystem; fast fluorescence kinetics; optical microcavity; fluorescence microscopy
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.