Edible films were produced combining pectin (P) matrix with chitosan nanoparticle (CSNP), polysorbate 80 (T80), and garlic essential oil (GEO) as an antimicrobial agent. The CSNP were analyzed for their size and stability, and the films, throughout their contact angle, scanning electron microscopy (SEM), mechanical and thermal properties, WVP and antimicrobial activity. Four filming-forming suspension were investigated: PGEO (control); PGEO@T80; PGEO@CSNP; PGEO@T80@CSNP. The average particle size was 317nm with zeta potential reaching +21.4 mV, which indicated colloidal stability. The wettability of the films exhibited values of 65°, 43°, 78°, 64° respectively. In antimicrobial tests, the films containing GEO showed inhibition only by contact for S.aureus. For E. coli, the inhibition occurred only in films containing CSNP and by direct contact in the culture. The results indicate a promising alternative for designing stable antimicrobial nanoparticles for application in novel food active packaging.