Tsiamis, A.; Buchoux, A.; Mahon, S.T.; Walton, A.J.; Smith, S.; Clarke, D.J.; Stokes, A.A. Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics. Micromachines2023, 14, 537.
Tsiamis, A.; Buchoux, A.; Mahon, S.T.; Walton, A.J.; Smith, S.; Clarke, D.J.; Stokes, A.A. Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics. Micromachines 2023, 14, 537.
Tsiamis, A.; Buchoux, A.; Mahon, S.T.; Walton, A.J.; Smith, S.; Clarke, D.J.; Stokes, A.A. Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics. Micromachines2023, 14, 537.
Tsiamis, A.; Buchoux, A.; Mahon, S.T.; Walton, A.J.; Smith, S.; Clarke, D.J.; Stokes, A.A. Design and Fabrication of a Fully-Integrated, Miniaturised Fluidic System for the Analysis of Enzyme Kinetics. Micromachines 2023, 14, 537.
Abstract
The lab-on-a-chip concept, enabled by microfluidic technology, promises the integration of multi-ple discrete laboratory techniques into a miniaturised system. Research into microfluidics has generally focused on the development of individual elements of the total system (often with rela-tively limited functionality), without full consideration for integration into a complete fully opti-mised and miniaturised system. Typically, the operation of many of the reported lab-on-a-chip devices is dependent on the support of a laboratory framework. In this paper, a demonstrator platform for routine laboratory analysis is designed and built, which fully integrates a number of technologies into a single device with multiple domains such as fluidics, electronics, pneumatics, hydraulics, and photonics. This facilitates the delivery of breakthroughs in research, by incorpo-rating all physical requirements into a single device. To highlight this proposed approach, this demonstrator microsystem acts as a fully integrated biochemical assay reaction system. The re-sulting design determines enzyme kinetics in an automated process and combines reservoirs, three-dimensional fluidic channels, optical sensing, and electronics in a low-cost, low-power and portable package.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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