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The Plagiochilins From Liverworts Plagiochila: Binding to α-Tubulin and Drug Design Perspectives

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19 April 2023

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Abstract
Among bryophytes, the Plagiochila genus represents a large group of leafy liverworts, with over 500 species. Plagiochilins A-to-X are sesquiterpenoids isolated from Plagiochila species. The lead compound plagiochilin A (Plg-A), endowed with anticancer and anti-parasitic properties, has been characterized as a potent mitosis inhibitor, acting selectively at the late stage of cytokinesis termed abscission. The compound perturbs the dynamic of microtubules, blocking cell cycle progression and triggering the death of malignant cells. Based on the compound mechanism of action and the analogy with other natural products bearing a dihydro-pyrone moiety, we postulated that Plg-A could bind to the pironetin site of α-tubulin. A molecular docking analysis has been performed to compare binding of all 24 plagiochilins to α-tubulin and to establish structure-binding relationships. The identification of Plg-E and Plg-G as the best binders in the series pointed out to the importance of the C13-OH or C=O group for α-tubulin recognition. This observation led to the testing of the natural product ester plagiochilin A-15-yl n-octanoate and the corresponding alcohol (Plg-OH), both identified as robust α-tubulin binders. The study provides a rational to explain potentially the mechanism of action of Plg-A and to guide the design of new derivatives.
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Subject: Biology and Life Sciences  -   Biochemistry and Molecular Biology

1. Introduction

Bryophytes were photosynthetic organisms among the first to colonize the terrestrial environment. The group includes mosses, hornworts and liverworts, found in nearly all terrestrial habitats on all continents [1]. The medicinal use of bryophytes dates to ancient times, with the first testimonies of their medical use reported in the 16th century [2]. A large chemical diversity of compounds can be isolated from bryophytes and liverworts in particular, including many terpenoids of prime pharmacological interest [3,4]. A large diversity of sesqui- and diterpenoids have been isolated and their antimicrobial, antifungal, cytotoxic, anti-inflammatory, and antiparasitic activities characterized [5]. We were particularly interested in bioactive natural products isolated from liverworts of the genus Plagiochila.
Plagiochilin A (Figure 1) is a secoaromadendrane-type sesquiterpenoid first isolated 45 years ago from the liverworts Plagiochila yokogurensis Steph. and Plagiochila hattoriana Inoue [6,7]. Later, the product was found in other Plagiochila species, such as P. semidecurrens, P. pulcherrima Horik., and P. disticha (Lehm. & Lindenb.) Lindenb. [8,9,10]. Plagiochilin A was initially characterized as an as a strong insect antifeedant agent, but this natural product displays a range of bioactivities including insecticidal, anti-inflammatory, anti-parasitic and anti-proliferative properties. Notably, the compound has been shown to reduce the growth of P-388 leukemia cells with a good efficacy (IC50 = 3.0 µg/ml) [11]. Another study demonstrated that Plg-A can inhibit the growth of a range of tumor cell lines, including prostate, breast, lung and leukemia cells. The compound proved particularly efficient at inhibiting the growth of DU145 prostate cancer cells, with an efficacy superior to reference anticancer drug fludarabine phosphate (GI50 = 3.0 µM) [10].
Plagiochilin A (hereafter designated Plg-A) is the leading member of a series of 24 derivatives, designated plagiochilins A-to-X (Figure 1), discovered over the past forty years. Plagiochilins are all natural products, isolated from diverse Plagiochila species from 1978 (Plg-A) to 2005 (Plg-X), as reviewed recently [12]. They are seco-aromadendrane-type sesquiterpenoids, considered as chemosystematic markers in the Plagiochilaceae [13]. The pharmacological properties of plagiochilins A and C have been investigated, but otherwise the other compounds have been rarely studied. A recent work has identified a key aspect of the mechanism of action of Plg-A. The compound was shown to block DU145 cell division by preventing completion of cytokinesis, stopping the cell cycle progression at the G2/M phase. Plg-A induced a blockade of cell division at the membrane abscission stage, which is the late stage of cytokinesis, thereby triggering cell apoptosis (Figure 2). The process is characterized by cytoskeletal effects, with a rearrangement of microtubules, implicating α-tubulin [14]. Cytokinetic abscission is a well-orchestrated, tightly regulated process which influences cell fate and tissue growth [15]. It implicates different proteins containing a microtubule-interacting and trafficking (MIT) domain. A microtubule-rich structure formed during cytokinesis, called the midbody, is a key regulator of the terminal stages of cell division [16]. Drugs affecting cytokinesis, such as PLK-1 (Polo-like kinase-1) and Aurora kinase inhibitors are considered for the treatment of cancers and new drugs capable of inhibiting cellular division are needed.
The molecular target of Plg-A is unknown at present, but based on previous studies, we considered that the natural product could bind to tubulin dimers, so as to block the recruitment of α- and β-tubulins for microtubule nucleation. The pironetin-binding site of α-tubulin is known to accommodate compounds bearing a dihydro-pyrone moiety, as found in plagiochilin Q for example [17,18]. These considerations prompted us to investigate the potential binding of plagiochilins to α-tubulin, using molecular modeling. Here we report a molecular docking analysis of all 24 plagiochilins to the pironetin site of α-tubulin, with the objective to identify the best potential binders in the series and to guide the development of plagiochilin analogues. The study identified plagiochilin G as the best α-tubulin-interacting compound in the series and provided important structure-binding relationships. Based on these observations, we can propose a rational mechanism of action for a highly potent hemisynthetic plagiochilin derivative discovered in the past [19,20]. The study provides novel perspectives to guide the design of tumor-active plagiochilin derivatives.

2. Materials and Methods

2.1. Molecular structure and software.

The molecular structure of α-tubulin interacting with pironetin was obtained from the Protein Data Bank (www.rcsb.org) under the PDB code 5FNV. It corresponds to a high-resolution structure (2.61 Å resolution) determined by X-ray diffraction [21]. We used the GOLD 5.3 package from the Cambridge Crystallographic Data Centre (Cambridge, UK) to software perform the docking experiments, with the support of the molecular graphic system provided by Discovery Studio Visualizer, Biovia 2020 (Dassault Systèmes BIOVIA Discovery Studio Visualizer 2020; San Diego, CA, USA, Dassault Systèmes, 2020). For the determination of the potential drug-binding sites on α-tubulin, we used the web server CASTp 3.0 (Computed Atlas of Surface Topography of proteins) which is well suited to define cavities on a protein surface and the specific amino acid positioning, important for the interaction. The updated CASTp web server is convenient to study protein surface architecture and functional regions with the key residues implicated in the interaction [22,23]. For analysis and visualization, the molecular modeling software Chimera 1.15 was used (https://www.cgl.ucsf.edu/chimera/).

2.2. Molecular modeling procedure.

For model building and evaluation, we used an iterative multi-step procedure. The BOSS software (Biochemical and Organic Simulation System, available at http://zarbi.chem.yale.edu/software.html) was used to perform a Monte Carlo (MC) conformational search of the different ligands [24]. Within BOSS, a general purpose molecular mechanics engine capable of performing various calculations, the MS simulation was realized in the isothermal-isobaric (NPT, constant number of particles, constant pressure, and constant temperature) ensemble. The analysis of the conformations defines the optimum geometry for each natural product. Thus, the minimum-energy conformers were identified through energy minimization. The next step refers to the calculation of the free energy of hydration (ΔG) for a given structure of the compound. The knowledge of ΔG is useful to investigate the origin of hydrophobic interactions. For this, the molecular mechanics/generalized Born surface area (MM/GBSA) procedure was used according to a described procedure [25]. MC search and computation of ΔG were performed within BOSS using the xMCGB script according to previously described procedures [25,26].
In our docking procedure, the pironetin-binding site on tubulin was defined as the ligand-binding site for all naturally-occurring plagiochilins and derivatives. With the 5FNV structure, the following flexible amino acids during the docking with GOLD procedure were defined: Phe135, Phe202, Leu248, Leu252, Phe255, Gln256, Leu259, Cys316, Lys352, and Leu378, based on shape complementarity criteria. Shape complementarity and geometry considerations favor a docking grid defined from the volume of the central amino acid. In our typical docking process, we used the ChemPLP scoring function to define the best 100 energetically favorable poses and search for the optimized binding mode of the test ligand. Each pose is analyzed and their ranking, aided by the PLP fitness scoring function available from GOLD version 5.3, helps to identify the best trial poses [27]. We usually keep up to six poses per compound and for the ranked complexes, the empirical potential energy of the interaction ΔE was evaluated using the equation ΔE(interaction) = E(complex) − [E(protein) + E(ligand)]. Energies are calculated on the basis of the SPASIBA (Spectroscopic Potential Algorithm for Simulating Biomolecular conformational Adaptability) spectroscopic force field, a convenient system to predict conformational relative energies, derived from vibrational wavenumbers obtained in the infrared and Raman spectra of a large series of compounds including organic molecules, amino acids, saccharides, nucleic acids and lipids. SPASIBA is essential to define the best protein-ligand structure. It has been specifically developed to provide refined empirical molecular mechanics force field parameters [28,29,30]. SPASIBA is integrated into CHARMM force field [31] which was found excellent at reproducing crystal-phase infrared data. Overall, this iterative procedure was used to build, analyze and compare the various drug-protein complexes.

3. Results

3.1. Comparative docking of plagiochilins to α-tubulin

The crystal structure of the reference natural product pironetin bound to an α/β-tubulin dimer was used as a template (PDB: 5FNV) to study the binding of plagiochilins to α-tubulin. Each of the 24 compounds was docked into the pironetin site and the most energetically favorable conformation was identified in each case. There is a small binding pocket in the center of the protein, sufficiently deep to accommodate completely a ligand like plagiochilin A, as represented in Figure 3. The compound is fully engaged into the cavity, inserting its epoxide unit deep into the pocket. All portions of the molecule contributed to the protein interaction, with notably three potential H-bonds with residues Ser241, Gln256 and Lys352, in addition to an array of van der Waals contacts and alkyl/π-alkyl interactions. The epoxide unit of Plg-A is interacting with Lys352 whereas the two acetyl groups are engaged in H-bonds with Ser241 and Gln256. Twenty-four similar models were built with the different plagiochilins. A specific model was elaborated for each compound, maintaining the same binding site. The 24 models were then analyzed and compared. For each compound and each model, the empirical energy of interaction (ΔE) and free energy of hydration (ΔG) were calculated (Table 1). Significant variations among the compounds were observed, from -37.9 kcal/mol for the weakest ligand Plg-N to -77.0 kcal/mol with the best compound Plg-G. The free energy of hydration (ΔG) also varied significantly from one compound to another. Calculated ΔG is a molecular descriptor which usually correlates reasonably well with experimental water solubility.
Plg-A can form stable complexes with α-tubulin, characterized by an energy of interaction (ΔE) of -63.8 kcal/mol, more favorable (more negative) than the energy calculated with the reference ligand pironetin. The tubulin binding capacity was found to vary significantly among the plagiochilins, as represented in Figure 4. Some plagiochilin derivatives such as Plg-C, H, J, N, Q, X and W only showed a weak tubulin binding capacity (ΔE< 50 kcal/mol). In contrast, the five compounds Plg-D, O, P, R and T revealed a tubulin binding aptitude similar or superior to that of Plg-A. A representative model of Plg-D bound to the pironetin site of α-tubulin is shown in Figure 5. This peracetylated compound can form apparently very stable complexes with the tubulin protein, stabilized by five H-bonding interactions with residues Ser241, Asn249, Asn258, Cys316 and Lys352, in addition to several weaker van der Waals contacts. The carbonyl groups are major elements of the drug-protein interaction. Plg-D differs from Plg-A by the addition of two acetyl groups at positions 12 and 13 of the seco-aromadendrane scaffold, but only the acetyl a position 12 is implicated in the protein interaction. A comparable situation was observed with Plg-P and R for which the addition of an -OAc substituent at position 13 led to a significant reinforcement of the tubulin binding capacity. However, the binding energy calculated with Plg-P is only 5% superior (more negative) to that obtained with Plg-A, a modest improvement.
Interestingly, two compounds emerge from the docking analysis, Plg-E and G with a binding capacity significantly superior to that of Plg-A in both cases. The best compound in the series is Plg-G with an empirical energy of interaction of -77 kcal/mol, 20% better than that of Plg-A. This compound fits well into the pironetin site, placing its bicyclic core at the bottom of the binding cavity and utilizing the acetyl groups for the protein anchorage. The OH group at C-13 is chiefly implicated in the interaction, via two major H-bonds with Ile238 and Ser241 (Figure 6). A similar model was obtained with Plg-E. This point has implications in term of drug design (see below). The epoxide unit of Plg-G also participates in the protein interaction via residue Lys352, as observed with Plg-A. No less than 24 molecular contacts between the compound and α-tubulin were identified in this case, including 4 potential H-bonds and 18 van der Waals contacts maintaining solidly the stability of the natural product-protein complex. The complex formed with Plg-E was similar, with key H-bonds with Ser241 and Lys352, and an array of van der Waals contacts (not shown). The oxygen atom of the two OAcetyl groups of Plg-E at C12 and C13 were both involved in the protein interaction. To sum up, the docking analysis supports the hypothesis that plagiochilins can form stable complexes with α-tubulin and suggests that two specific derivatives, plagiochilins E and G, are particularly well adapted to bind to the pironetin site of α-tubulin.

3.2. Drug design implications

The docking analysis helped us to identify drug substituents of the plagiochilin scaffold important for protein binding. The epoxide moiety of Plg-A, together with its two OAc substituents contribute significantly to the protein interaction, as represented in Figure 3c. But the main discovery is the possibility to reinforce significantly the interaction when a hydroxyl or acetyl group is introduced at positions C-12 and/or C-13. The optimal configuration was observed with Plg-G bearing a C-13 OH group. Based on this observation, we searched the literature for plagiochilin derivatives possibly bearing such a functional group. We could not directly find such as compound but interestingly, we identified the derivative plagiochilin A-15-yl n-octanoate (Figure 7) which has been presented many years ago as a highly active anti-proliferative compound, 60-times more potent against P-388 leukemia cells than the parent compound Plg-A (ID50 = 0.05 and 3.0 µg/mL, respectively) [18]. This compound is an ester, with an octanoate side chain which may serve as a lipophilic carrier to enhance the bioavailability of the compound. Such ester derivatives are generally unstable in vivo and can hydrolyze to release the corresponding acid (caprylic acid in the present case) and the alcohol derivative which we have designated here Plg-OH (Figure 7). Therefore, we modeled these two plagiochilin derivatives in interaction with α-tubulin, to determine the potential contribution of the C13-OH or C=O group to the protein interaction.
The ΔE value calculated with Plg-OH is intermediate between that of Plg-E and Plg-G (Table 1 and Figure 4). The binding configuration superimposed well with that of Plg-G, implicating the same H-bonds with residues Ile238 and Ser241, Lys352. The two models were very similar (not shown). Therefore, the alcohol derivative Plg-OH could be used as a starting point to design other esters, ethers or different entities. In addition, surprisingly we observed that the elongated compound bearing the octanoate side chain could also fit well into the binding cavity. The ΔE value for PlgA-octanoate (-77.5 kcal/mol) is identical to that of Plg-G, the best compound in the series. The presence of the long alkyl chain does not prevent drug binding, but contributes to the stability of the drug-protein complex. The model in Figure 8 shows the folding of the compound, placing the alkyl chain into the cavity, in interaction with the protein via van der Walls and alkyl contacts. The interaction of PlgA-octanoate with α-tubulin may contribute, at least partially, to its potent anti-proliferative activity.

4. Discussion

Bryophytes (non-vascular plants), are extremely abundant and diversified. The group includes three main subcategories: Bryophyta (mosses), Marchantiophyta (liverworts) and Anthocerotophyta (hornworts). More than 7400 species of liverworts and hornworts have been inventoried [32]. The genus Plagiochila refer to one of the most important groups of leafy liverworts with >500 species distributed on all continents and territories [33]. Yet, Plagiochilaceae have been little studied thus far. They represent a potential source of bioactive compounds, such as the plagiochilins studied here, but also sesquiterpenoids (plagicosins) and alkaloids (plagiochianins), as discussed recently [7].
The series of plagiochilins has been characterized over the past 30 years, mainly from a natural product discovery perspective. The 24 derivatives were successively isolated, structurally identified, but their biological and pharmacological properties have not been significantly investigated. Anti-parasitic and anti-cancer effects have been reported essentially for Plg-A and Plg-C [10,19,34,35,36,37]. The other compounds have been somewhat neglected at present. But recently, a major study has provided key element to understand the mechanism of action of the lead product Plg-A. The product has been identified as a potent inhibitor of cytokinesis and its anti-mitotic effects are likely at the origin of the anti-proliferative action of the compound [14]. Plg-A was shown to induce specific mitotic figures, with accumulation of dividing DU145 cells connected with intercellular bridges, corresponding to abscission stage of cytokinesis. By so doing, the natural product reduced the number and size of DU145 cell colonies and induced cell death [14]. Plg-A exerts an effect on the cytoskeleton, with a rearrangement of α-tubulin characteristic of cytokinetic membrane abscission. Based on these observations, we postulated that the compound can alter the microtubule-organizing center which recruits α- and β-tubulins for microtubule nucleation. We postulated that the compound can bind the pironetin-binding site of α-tubulin, which is known to accommodate compounds bearing a dihydro-pyrone moiety [17,18]. This moiety can be found in plagiochilin Q and recently, we showed that a related series of natural products with a 5,6-dihydro-α-pyrone unit, the cryptoconcatones, can function as α-tubulin-binding agents. Notably, we identified cryptoconcatones F and L as robust α-tubulin binders capable of forming covalent protein adducts [38]. We also evidenced the cytotoxic lactone spicigerolide with a pyranone moiety as a tubulin binder. These considerations prompted us to investigate the binding of plagiochilins to α-tubulin.
The molecular modeling analysis suggests that several plagiochilin derivatives can form stable complexes with α-tubulin, via binding to the pironetin site. The best potential binders are Plg-E and Plg-G which are structurally similar. The analysis points to the key role of the C13-OH or O-C=O group in the interaction with tubulin. The importance of this unit was further investigated with the two compounds Plg-OH and plagiochilin A-15-yl n-octanoate, both identified as potent α-tubulin binding agents. The observation is important to guide future drug design in the series. Analogues bearing different ester groups or other side chains could be designed. The study opens novel perspectives for the discovery and design of novel plagiochilin-like molecules. The difficulty is to obtain the starting materials for synthesis. Thus far, only the total (and difficult, 16-steps) synthesis of Plg-N has been reported, starting from the anthelmintic sesquiterpene lactone santonin [39]. However, there exist options to obtain sufficient quantities of Plg-A to perform for hemisyntheses. Plagiochila biomass can be produced under laboratory conditions through bryo-reactors and molecular farming [40]. Through such biotechnological processes, secondary metabolites from bryophytes can be produced in large quantity, leading to the exploitation of their pharmaceutical properties. A new era of bryo-pharmaceuticals is put into perspective [41]. Bryophytes become increasingly considered as a source of medicinal products, notably as antifungal agents [3].
With no doubt, Plagiochila species warrant further investigations as a source of bioactive compounds. Different molecules of biopharmaceutical interest have been isolated recently from various species such P. porelloides which afforded novel antiparasitic compounds [42]. A series of terpenoids designated plagicosins A-N, isolated from the Chinese liverwort P. fruticosa Mitt., has been characterized, with a lead compound plagicosin F inhibiting the adhesion and biofilm formation of the fungus Candida albicans [43]. There are also interesting macrocyclic bisbibenzyl compounds, such as isoplagiochins C and D from P. fruticosa and other species [44,45]. Plagiochilins, notably the lead molecules plagiochilin A and plagiochilin G are emerging as an interesting series of anticancer agents. Plg-G has been isolated more than 40 years ago from P. ovalifolia Mitt. [34], the same species that produced Plg-N [39], and plagiochilin-A-15-yl octanoate [19]. P. ovalifolia is an eastern Asiatic species, so far known essentially known from China, Japan, Korea and Philippines [46]. It is possible to establish suspension culture from callus tissue induced by culturing spores of P. ovalifolia [47]. Therefore, there is a possibility to generate plagiochilin derivatives. Hopefully, our docking analysis will promote researches on Plagiochila and plagiochilins. Beyond the plagiochilins, the work reinforces the interest of targeting α-tubulin and the pironetin site, still considered an under-explored target for cancer therapeutics [18]. The present study adds a few new compounds to the list of natural products with microtubules destabilizing properties. The design of novel plagiochilin-A derivatives shall be highly encouraged.

Author Contributions

(CRediT roles): GV: Visualization; Software; Computations; Molecular modeling. CB: Conceptualization; Investigation; Visualization; Writing—original draft; Writing—review & editing.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data sharing not applicable.

Acknowledgments

The OncoLille institute has been supported by a grant from Contrat de Plan Etat-Région CPER Cancer 2015-2020.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Structures of plagiochilins.
Figure 1. Structures of plagiochilins.
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Figure 2. Cytokinesis inhibition by plagiochilin A (Plg-A). At the telophase stage of mitosis, prior to cell division, the two cells are connected by an intercellular bridge with a central midbody. Plg-A prevents completion of cytokinesis at the final abscission stage, so as to block cell division. The drug-induced inhibition of the late stage of cytokinesis leads to cell cycle arrest (G2/M) and subsequent induction of apoptotic cell death [14].
Figure 2. Cytokinesis inhibition by plagiochilin A (Plg-A). At the telophase stage of mitosis, prior to cell division, the two cells are connected by an intercellular bridge with a central midbody. Plg-A prevents completion of cytokinesis at the final abscission stage, so as to block cell division. The drug-induced inhibition of the late stage of cytokinesis leads to cell cycle arrest (G2/M) and subsequent induction of apoptotic cell death [14].
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Figure 3. Molecular model of Plg-A bound to α-tubulin (PDB: 5FNV). (a) A ribbon model of α-tubulin with bound Plg-A. The α-helices (in red) and β-sheets (in cyan) are shown. (b) A close-up view of Plg-A bound to the pironetin site. (c) A representation of the main Plg-A/α-tubulin contacts (H-bonds) to the indicated amino acid residues.
Figure 3. Molecular model of Plg-A bound to α-tubulin (PDB: 5FNV). (a) A ribbon model of α-tubulin with bound Plg-A. The α-helices (in red) and β-sheets (in cyan) are shown. (b) A close-up view of Plg-A bound to the pironetin site. (c) A representation of the main Plg-A/α-tubulin contacts (H-bonds) to the indicated amino acid residues.
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Figure 4. Representation of the empirical energies of interaction (ΔE) calculated with each plagiochilin derivative and the reference compound pironetin (Pr). Values obtained with the derivatives Plg-OH and plagiochilin A-15-yl n-octanoate (plg-octanoate) are also pointed out. The top part of the figure shows the structures of the aromadendrane and 2,3-seco-aromadendrane scaffolds (with the numbering scheme) and the structural analogy between the two best compounds Plg-E and G.
Figure 4. Representation of the empirical energies of interaction (ΔE) calculated with each plagiochilin derivative and the reference compound pironetin (Pr). Values obtained with the derivatives Plg-OH and plagiochilin A-15-yl n-octanoate (plg-octanoate) are also pointed out. The top part of the figure shows the structures of the aromadendrane and 2,3-seco-aromadendrane scaffolds (with the numbering scheme) and the structural analogy between the two best compounds Plg-E and G.
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Figure 5. Molecular model of Plg-D bound to α-tubulin. (a) A surface model to show that Plg-D fits into a central, deep cavity of the protein. (b) A detailed view of the Plg-G binding site, with the H-bond donor/acceptor groups surrounding the drug binding zone (color code indicated). (c) Binding map contacts for Plg-D bound to α-tubulin (color code indicated).
Figure 5. Molecular model of Plg-D bound to α-tubulin. (a) A surface model to show that Plg-D fits into a central, deep cavity of the protein. (b) A detailed view of the Plg-G binding site, with the H-bond donor/acceptor groups surrounding the drug binding zone (color code indicated). (c) Binding map contacts for Plg-D bound to α-tubulin (color code indicated).
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Figure 6. Molecular model of Plg-G bound to α-tubulin. (a) Surface model with a close-up view of the binding cavity which accommodates the compound. (b) A view of the Plg-G binding site, with the solvent-accessible surface (SAS) surrounding the drug binding zone (color code indicated). (c) Binding map contacts for Plg-G bound to α-tubulin (color code indicated).
Figure 6. Molecular model of Plg-G bound to α-tubulin. (a) Surface model with a close-up view of the binding cavity which accommodates the compound. (b) A view of the Plg-G binding site, with the solvent-accessible surface (SAS) surrounding the drug binding zone (color code indicated). (c) Binding map contacts for Plg-G bound to α-tubulin (color code indicated).
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Figure 7. Structure of the hemisynthetic derivative plagiochilin A-15-yl n-octanoate and its suspected hydrolysis to release the alcohol derivative Plg-OH and the corresponding acid.
Figure 7. Structure of the hemisynthetic derivative plagiochilin A-15-yl n-octanoate and its suspected hydrolysis to release the alcohol derivative Plg-OH and the corresponding acid.
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Figure 8. A model of plagiochilin A-15-yl n-octanoate bound to the pironetin site of α-tubulin (a) and the corresponding contact map (b).
Figure 8. A model of plagiochilin A-15-yl n-octanoate bound to the pironetin site of α-tubulin (a) and the corresponding contact map (b).
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Table 1. Potential energy of interaction (ΔE) and free energy of hydration (ΔG) for the interaction of plagiochilins with α-tubulin.
Table 1. Potential energy of interaction (ΔE) and free energy of hydration (ΔG) for the interaction of plagiochilins with α-tubulin.
Compounds ΔE (kcal/mol) ΔG (kcal/mol)
Plagiochilin A -63.80 -19.90
Plagiochilin B -62.30 -26.00
Plagiochilin C -47.80 -18.55
Plagiochilin D -67.30 -19.10
Plagiochilin E -71.00 -17.20
Plagiochilin F -59.30 -23.15
Plagiochilin G -77.00 -26.00
Plagiochilin H -44.00 -21.30
Plagiochilin I -53.25 -16.80
Plagiochilin J -42.40 -18.40
Plagiochilin K -52.10 -15.50
Plagiochilin L -57.60 -22.70
Plagiochilin M -53.70 -21.20
Plagiochilin N -37.90 -15.10
Plagiochilin O -64.40 -23.70
Plagiochilin P -66.85 -24.00
Plagiochilin Q -39.30 -17.30
Plagiochilin R -66.50 -25.50
Plagiochilin S -53.40 -20.70
Plagiochilin T -63.10 -23.30
Plagiochilin U -60.40 -20.90
Plagiochilin V -57.45 -16.10
Plagiochilin W -46.60 -19.90
Plagiochilin X -41.30 -20.00
PlgA-octanoate -77.50 -28.40
Plg-OH -72.40 -22.20
Pironetin -57.32 -24.20
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