Review
Version 1
Preserved in Portico This version is not peer-reviewed
Flow Cytometry of Oxygen and Oxygen-Related Cellular Stress
Version 1
: Received: 29 April 2023 / Approved: 30 April 2023 / Online: 30 April 2023 (03:08:04 CEST)
A peer-reviewed article of this Preprint also exists.
Jávega, B.; Herrera, G.; Martínez-Romero, A.; O’Connor, J.-E. Flow Cytometry of Oxygen and Oxygen-Related Cellular Stress. Oxygen 2023, 3, 222-255. Jávega, B.; Herrera, G.; Martínez-Romero, A.; O’Connor, J.-E. Flow Cytometry of Oxygen and Oxygen-Related Cellular Stress. Oxygen 2023, 3, 222-255.
Abstract
Oxidative stress has been implicated in cellular senescence and aging, as well as in the onset and progression of many diverse genetic and acquired diseases and conditions. The search for biomarkers of oxidative stress has become relevant to many biomedical fields using many different methods and approaches. Fluorescence methodology is advantageous because of its simplicity and high sensitivity. Fluorescent probes are frequently used to investigate the role of reactive oxygen species in oxidative stress in experimental or clinical settings, often involving the use of flow cytometry and related single-cell based technologies. To assess the specific role of reactive oxygen species in oxidative stress studies by cytometric methodologies, it is essential to detect and characterize these species accurately. However, the detection and quantitation of individual intracellular ROS is still a challenge, but different and complementary strategies may focus on other endpoints of oxidative stress. In this review we present and discuss briefly the limitations and perspectives of such approaches. Based on our results, we also provide recommendations for proper design of cytometric studies of oxidative stress in order to prevent or minimize experimental errors..
Keywords
Fluorescence; Image Cytometry; Cytomics; Reactive Oxygen Species; Antioxidants; Oxydative Stress; In Vitro Toxicology
Subject
Biology and Life Sciences, Toxicology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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