Preprint Review Version 2 Preserved in Portico This version is not peer-reviewed

Cell-type-specific neuroproteomics of synapses

Version 1 : Received: 18 May 2023 / Approved: 19 May 2023 / Online: 19 May 2023 (15:40:25 CEST)
Version 2 : Received: 10 June 2023 / Approved: 13 June 2023 / Online: 13 June 2023 (10:44:42 CEST)

A peer-reviewed article of this Preprint also exists.

Yim, Y.Y.; Nestler, E.J. Cell-Type-Specific Neuroproteomics of Synapses. Biomolecules 2023, 13, 998. Yim, Y.Y.; Nestler, E.J. Cell-Type-Specific Neuroproteomics of Synapses. Biomolecules 2023, 13, 998.

Abstract

In the last two decades, our knowledge of synaptic proteomes and their relationship to normal brain function and neuropsychiatric disorders has been expanding rapidly through the use of more powerful neuroproteomic approaches. However, mass spectrometry (MS) based neuroproteomic studies of synapses still need cell-type, spatial, and temporal proteome information. With the advancement of sample preparation and MS techniques, we have just begun to identify and understand proteomes within a given cell type, subcellular compartment, and cell-type-specific synapse. Here, we review the progress and limitations of MS-based neuroproteomics of synapses in the mammalian CNS and highlight the recent applications of these approaches in studying neuropsychiatric disorders such as major depressive disorder and substance use disorders. Combining neuroproteomic findings with other omics studies can generate an in-depth, comprehensive map of synaptic proteomes and possibly identify new therapeutic targets and biomarkers of several central nervous system disorders.

Keywords

Neuroproteomics, synapse, neurological and psychiatric disorders, cell-type specificity

Subject

Biology and Life Sciences, Neuroscience and Neurology

Comments (1)

Comment 1
Received: 13 June 2023
Commenter: Yun Young Yim
Commenter's Conflict of Interests: Author
Comment: This version is an updated version of the manuscript to correct errors in the previous version. 
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Comment 2
Received: 15 June 2023
Commenter: Matt Rowan
The commenter has declared there is no conflict of interests.
Comment: Wonderful review! I wanted to point you to our very recent TurboID preprint currently under review, which isolated the native-state in vivo protome of PV interneurons in both WT and Alzheimer's models. We would certainly appreaciate it if you would consider mentioning our work here during your revision.

https://www.biorxiv.org/content/10.1101/2023.05.17.541038v1
All the best,
Matt Rowan
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