Article
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Preserved in Portico This version is not peer-reviewed
Research on the Device of Micro Droplet PCR in Microfluidic Chip Based on Constant Pressure Regulation
Version 1
: Received: 29 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (10:31:02 CEST)
A peer-reviewed article of this Preprint also exists.
Duanmu, L.; Yu, Y.; Meng, X. Microdroplet PCR in Microfluidic Chip Based on Constant Pressure Regulation. Micromachines 2023, 14, 1257. Duanmu, L.; Yu, Y.; Meng, X. Microdroplet PCR in Microfluidic Chip Based on Constant Pressure Regulation. Micromachines 2023, 14, 1257.
Abstract
The device and method in accordance with the constant pressure regulation of micro droplet PCR in microfluidic chips are developed to explore the optimization way for the micro droplet movement, fragmentation, and bubble generation in microfluidic chips in existing digital PCR technology. In the developed device, an air source device is adopted to regulate the pressure in the chip, such that micro droplet generation and PCR amplification without bubbles can be achieved. In 3 min, the sample in 20ul will be distributed into nearly 50,000 water-in-oil droplets exhibiting a diameter of about 87μm, and the micro droplet will be subjected to a close arrangement in the chip without air bubbles. The device and chip are adopted to quantitatively detect the human gene . As indicated by the experimental results, a good linear relationship exists between detection signal and DNA concentration ranging from 101~ 105 copies /μL (R2=0.999). The micro droplet PCR devices based on constant pressure regulation chips exhibit a wide variety of advantages (e.g., achieving high pollution resistance, micro droplet fragmentation and integration avoidance, reducing human interference, and standardizing results). Thus, micro droplet PCR devices based on constant pressure regulation chips have promising applications in nucleic acid quantification.
Keywords
Constant Pressure Regulation; Micro droplet Digital PCR; Fluorescence Detection; Quantitative Detection
Subject
Engineering, Control and Systems Engineering
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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