da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582.
da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582.
da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582.
da Costa, N.M.M.; Parisi, L.; Ghezzi, B.; Elviri, L.; Scombatti de Souza, S.L.; Novaes Júnior, A.B.; de Oliveira, P.T.; Macaluso, G.M.; Palioto, D.B. Anti-Fibronectin Aptamer Modifies Blood Clot Pattern and Stimulates Osteogenesis: An Ex Vivo Study. Biomimetics 2023, 8, 582, doi:10.3390/biomimetics8080582.
Abstract
Background: Scaffold (SCA) functionalization with aptamers (APT) provides adsorption of specific bioactive molecules on biomaterials surface. The aim of this study was to observe if SCA enriched with anti-fibronectin APT can favor coagulum (PhC) and osteoblasts (OSB) differentiation. Methods: 20 ug of APT was functionalized on SCA by simple adsorption. For PhC formation, SCAs were inserted into rat calvaria defects for 17 hours. Following proper transportation (Buffer solution-PB), OSB (UMR-106 lineage) were seeded over PhC + SCAs with and without APT. Cells and PhC morphology, PhC cell population, protein labelling and gene expression were observed in different time points. Results: The APT induced higher ALP and BSP immunolabeling in OSB. CD90, CD45 and CD44 expression was more detected in APT group than when scaffolds were not functionalized. Additionally, an enriched and dense fibrin network and different cell types were observed, with more OSB and white blood cells in PhC formed on SCA with APT. The gene expression showed higher TGF-β1 detection in SCA with APT. Conclusions: The SCA functionalization with fibronectin aptamers provides a selective expression of proteins related to coagulation pattern and osteo differentiation. Additionally, aptamers increase TGF-β1 gene expression, which is highly associated with improvements in regenerative therapies.
Keywords
Aptamer; Blood Clot; Three dimensional; Scaffold; Hydrogel; Osteogenesis.
Subject
Chemistry and Materials Science, Biomaterials
Copyright:
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