Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multiplex Portable Biosensor for Bacteria Detection

Version 1 : Received: 12 September 2023 / Approved: 13 September 2023 / Online: 14 September 2023 (08:53:53 CEST)

A peer-reviewed article of this Preprint also exists.

Kaci, K.; Enebral-Romero, E.; Martínez-Periñán, E.; Garrido, M.; Pérez, E.M.; López-Diego, D.; Luna, M.; González-de-Rivera, G.; García-Mendiola, T.; Lorenzo, E. Multiplex Portable Biosensor for Bacteria Detection. Biosensors 2023, 13, 958. Kaci, K.; Enebral-Romero, E.; Martínez-Periñán, E.; Garrido, M.; Pérez, E.M.; López-Diego, D.; Luna, M.; González-de-Rivera, G.; García-Mendiola, T.; Lorenzo, E. Multiplex Portable Biosensor for Bacteria Detection. Biosensors 2023, 13, 958.

Abstract

An advanced, cost-effective and portable DNA biosensor capable of detecting multiple bacteria simultaneously have been developed. The biosensor consists of a fast and inexpensive potentiostat that controls the applied potential to a screen-printed electrochemical array platform functionalized with MoS2 flakes and bacteria DNA probes, and monitors the current response obtained by the use of à la carte thionine functionalized carbon nanodots (Ty-CDs) as electrochemical indicator of the hybridization event. The design of the potentiostat prioritizes achieving an optimal signal-to-noise ratio and incorporates a user-friendly interface compatible with various devices such as computers, mobile phones and tablets. The device is compact, lightweight, and manufactured at a low cost. The key components of the potentiostat include a data acquisition board capable of analyzing multiple samples simultaneously and a controller board. The results obtained confirm the ability of the developed device to successfully detect specific bacteria DNA sequences, demonstrating the reliability and performance of the developed multiplexing portable biosensor with better sensibility than using a traditional, more complex and laboratory oriented potentiostat.

Keywords

DNA biosensor, functionalized carbon nanodots, multiplex potentiostat, bacteria detection

Subject

Chemistry and Materials Science, Analytical Chemistry

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.