Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Reproducible Method for 1-methylcylopropene (1-MCP) Application and Quantitation for Post-harvest Research

Version 1 : Received: 11 October 2023 / Approved: 11 October 2023 / Online: 12 October 2023 (05:35:48 CEST)

A peer-reviewed article of this Preprint also exists.

Stowe, E.; Mattinson, D.S.; Blauer, J.M.; Dhingra, A. Reproducible Method for 1-Methylcylopropene (1−MCP) Application and Quantitation for Post-Harvest Research. Horticulturae 2024, 10, 5. Stowe, E.; Mattinson, D.S.; Blauer, J.M.; Dhingra, A. Reproducible Method for 1-Methylcylopropene (1−MCP) Application and Quantitation for Post-Harvest Research. Horticulturae 2024, 10, 5.

Abstract

Actively combating post-harvest food spoilage and waste can dramatically increase the efficiency of food utilization worldwide. In climacteric fruits, chemical treatments such as 1-MCP are an effective way of reducing post-harvest spoilage and waste by inhibiting the fruit’s ability to perceive ethylene. 1-MCP treatment is increasingly being used to explore the complex nature of ripening physiology at a fundamental level, however, differences in application and quantitation methods create difficulties in comparing conclusions across studies. Here we report an effective and reproducible method for 1-MCP application and quantitation for small-medium sized research applications. By use of surrogate alkene standards 1-butene and cis-2-butene, the highly volatile and elusive 1-MCP molecule can be identified and quantified by gas chromatography and subsequent standard curves may be developed. It is hoped that the methodology outlined here can help standardize consistent 1-MCP application for post-harvest research without excessive investment in specialized equipment.

Keywords

fruit ripening; post-harvest; ethylene; 1-methylcylcopropene

Subject

Biology and Life Sciences, Horticulture

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