Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to Fusarium graminearum Infection

Version 1 : Received: 20 October 2023 / Approved: 20 October 2023 / Online: 23 October 2023 (09:43:00 CEST)

A peer-reviewed article of this Preprint also exists.

Zhang, J.; Shi, H.; Yang, Y.; Zeng, C.; Jia, Z.; Ma, T.; Wu, M.; Du, J.; Huang, N.; Pan, G.; Li, Z.; Yuan, G. Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to Fusarium graminearum Infection. J. Fungi 2023, 9, 1157. Zhang, J.; Shi, H.; Yang, Y.; Zeng, C.; Jia, Z.; Ma, T.; Wu, M.; Du, J.; Huang, N.; Pan, G.; Li, Z.; Yuan, G. Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to Fusarium graminearum Infection. J. Fungi 2023, 9, 1157.

Abstract

Gibberella ear rot (GER) caused by Fusarium graminearum (teleomorph Gibberella zeae) is one of the most destructive diseases in maize that severely reduces grain yield and contaminates several potential mycotoxins. However, few efforts had been devoted to dissect the genetic basis of maize GER resistance. In the present study, a genome-wide association study (GWAS) was conducted in a maize association panel consisting of 303 diverse inbred lines. The phenotypes of GER severity were evaluated using kernel bioassay across multiple time points in the laboratory. Then, three models including fixed and random model circulating probability unification model (FarmCPU), general linear model (GLM) and mixed linear model (MLM), were conducted simultaneously in GWAS to identify single-nucleotide polymorphisms (SNPs) significantly associated with GER resistance. A total of four individual significant association SNPs with the phenotypic variation explained (PVE) ranging from 3.51 to 6.42% were obtained. Interestingly, the peak SNP (PUT-163a-71443302-3341) with the greatest PVE value, was co-localized in all models. Subsequently, 12 putative genes were captured from the peak SNP that several of these genes were directly or indirectly involved in disease resistance. Overall, these findings contribute to understand the complex plant-pathogen interactions in maize GER resistance. The regions and genes identified herein provide a list of candidate targets for further investigation, in addition to the kernel bioassay that can be used for evaluating and selecting elite germplasm resources with GER resistance in maize.

Keywords

maize; Gibberella ear rot; Fusarium graminearum; genome-wide association study; kernel bioassay

Subject

Biology and Life Sciences, Plant Sciences

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