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STAT3 Inhibitory Activities of Lignans Isolated from the Stems of Lindera obtusiloba Blume

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24 October 2023

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24 October 2023

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Abstract
Lindera obtusiloba Blume has several activities, such as anti-inflammatory, anti-allergic, anti-tumor, anti-wrinkle, and antioxidant activities. Interleukin-6 (IL-6) is a classic pro-inflammatory cytokine that is associated with various functions, such as proliferation, invasion, inflammatory responses and functions within antioxidant defence systems. In this study, we investigated IL-6-induced STAT3 activation of lignan compounds isolated from L. obtusiloba. The structures of the isolated compounds were elucidated via spectroscopic nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI-MS). As a result, seven lignans were identified from L. obtusiloba. All the isolated compounds (1-7) were evaluated for their IL-6-induced STAT3 inhibitory effects in Hep3B cells using a luciferase reporter assay. Of the isolates, compounds 1 and 5 showed inhibitory effects against IL-6-stimulated STAT3 activation. Furthermore, the mRNA expression levels of inflammation-related genes such as CRP, IL-1b, and SOCS3 were significantly reduced by exposure to compound 1 and 5. The protein levels of p-STAT3, p-JAK2 in IL-6 induced U266 cells were regulated in the presence of lignans derived from Lindera obtusiloba by western blot assay. Based on the results, this study of L. obtusiloba demonstrates that the species has promise as a bioactive candidate for the treatment of IL-6-induced STAT3-related disease.
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Subject: Biology and Life Sciences  -   Life Sciences

1. Introduction

Lindera obtusiloba Blume, a flowering plant species and deciduous broad-leaved tall tree belonging to Lauraceae, is a ubiquitous tree found mainly in Korea, Japan, and China. This plant is a known medical herb traditionally used for treating fever, abdominal pain, extravasation, and inflammation, improving of blood circulation, and preventing liver damage [1,2]. Previous phytochemical studies revealed that this medicinal plant contains several secondary metabolites, such as lignans [4], neolignans, flavonoids [3], and butanolides [5]. Extracts of this plant have been reported to have anti-tumour, anti-allergy, anti-inflammatory, anti-wrinkle, antioxidant, whitening, antiplatelet, antithrombotic, vasoprotective and antihypertensive effects [4,6,7,8,9,10]. Previous articles showed that L. obtusiloba contained daucosterol, leonuriside A and 3,4-dihydroxyphenethyl glycoside from xyle [11], germacrene B, β-caryophyllene, phytol isomer and (-)-β-elemene [12], flavonoid derivatives quercitrin and hyperoside [3], essential oil such as monoterpenoid and sesquiterpenoid [12] from leaves, secoisolariciresinol [13] and lignan derivatives (+)-syringaresinol and actifolin from stem [4].
Interleukin-6 (IL-6) is a classic pro-inflammatory cytokine important in normal cell inflammatory processes [17] and not only contributes to cancer-related inflammation but also plays crucial roles in DNA damage repair, antioxidant defence systems, proliferation, invasion, metastasis, angiogenesis and metabolic remodeling [18]. IL-6 signaling is initiated by binding IL-6 to IL-6 receptor (IL-6R) complexes, which are associated with IL-6, IL-6Ra, and gp130 receptor chains, and its activation leads to the JAK-STAT and MAPK signaling pathways [19]. Further, the activation of STAT3, which forms phosphorylated dimers that can translocate into the nucleus, promotes the expression of inflammation-related gene, such as C-reactive protein (CRP), The IL-6-induced JAK2/STAT3 signaling pathway plays a positive role in inflammation and neoplasia. The phosphorylation of JAK2 and STAT3 leads to dimerization of STAT3 and translocation to the nucleus [20].
In this study, we described for chemical structure of seven lignans from the stems of Lindera obtusiloba Blume on the basis of spectroscopic data such as nuclear magnetic resonance (NMR) and electrospray-ionization mass spectrometry (ESI-MS) as well as evaluated for inhibitory activities on IL-6/STAT3 activation of all isolated compounds.

2. Materials and Methods

2.1. General experimental procedures

1H, 13C and 2D NMR spectra were generated using a JEOL JNM-ECA 400 and JEOL JNM-ECA600 instruments (JEOL, Tokyo, Japan) using TMS as an internal standard. Optical rotations were obtained on a Jasco P-2000 polarimeter (Jasco Corp., Tokyo, Japan). High resolution election spray ionization mass spectrometry (HRESIMS) data were acquired using a Waters SYNAPT G2-Si HDMS spectrometer (Waters, Milford, MA, USA). Column Chromatography (C.C) was performed using Silica gel (Kieselgel 60, 200 – 400 mesh, Merck, Darmstadt, Germany) and Sephadex LH-20 (GE Healthcare, Uppsala, Sweden). Each fraction was monitored by TLC profiling using silica gel 60 F254 and RP-18 F254s (Merck, Burlington, USA) and medium-pressure liquid chromatography (MPLC, CombiFlash RF, Teledyne lsco, Lincoln, NE, USA) were used to separate the fractions of the extract. semipreparative high-speed liquid chromatography (semipreparative HPLC) was conducted using a Shimadzu LC-6AD instrument (Shimadzu Corp., Tokyo, Japan) equipped with an SPD-20A detector and Phenomenex Luna C18 (21.2mm × 250 mm, 5µm) column. All fractions and compounds were analyzed using an Agilent 1200 series HPLC system (Agilent, Santa Clara, USA).

2.2. Extraction and isolation

Dried stem of Lindera Obtusiloba Blume was extracted with 50% EtOH 200L at 70℃ (5h, ×10). After filtering(No. 10, 600mm, Hyundai Micro Co., Seoul, South Korea), the filtrates were concentrated under reduced pressure to obtain 554.3g of extract. The residue (500 g) was suspended in distilled water (5 L), and the aqueous layer was partitioned with n-hexane, EtOAc and BuOH. The EtOAc layer (71.24 g) was subjected to silica gel colulmn eluted with n-hexane:EtOAc (1:0 → 0:1, v/v), EtOAc:MeOH (9:1 → 0:1, v/v) to obtain 10 fractions (EA1 ~ EA10). Fraction EA5 (801.3 mg) was subjected to a Sephadex LH-20 column eluted with MeOH to yield 7 subfractions (EA5-1~7). Fraction EA5-6 (81.1 mg) was subjected to a Sephadex LH-20 column eluted with MeOH and further purified by preparative HPLC (Phenomenex Luna C18 column 250 × 21.2mm, 5µ), and isocratic elution with 50% CH3CN in H2O to afford compound 1 (11.1mg). Compound 4 (2.2 mg) was obtained from fraction EA5-2 (93.5 mg) by preparative HPLC (Phenomenex Luna C18 column 250 × 21.2mm, 5µ), using isocratic elution with 40% CH3CN in H2O. Fraction EA7 (3.27 g) was subjected to silica gel column eluted with CHCl3:MeOH (50:1 → 0:1, v/v) to yield 15 subfractions (EA7-1~15). Fraction EA7-5 (152.5 mg) and Fraction EA7-8 (200.1 mg) was further purified by preparative HPLC (Phenomenex Luna C18 column 250 × 21.2mm, 5µ), and isocratic elution with 30% and 40% CH3CN in H2O to afford compounds 3 (4.5 mg) and 5 (5.7 mg). Compound 6 (2.7 mg) was obtained from fraction EA7-12 (104.5 mg) by preparative HPLC (Phenomenex Luna C18 column 250 × 21.2mm, 5µ), using isocratic elution with 20% CH3CN in H2O. Fraction EA8 (10.45 g) was subjected to MPLC [column: Silica RediSepRf (40 g); mobile phase: CHCl3:MeOH (50:1 → 0:1, v/v)] to yield 20 subfractions (EA8-1~20). Fraction EA8-13 was purified by preparative HPLC (Phenomenex Luna C18 column 250 × 21.2mm, 5µ), using isocratic elution with 30% and 35% CH3CN in H2O to obtain compound 7 (2.2 mg) and compound 2 (4.7mg).
Episesamin (1) : white amorphous powder, ESI-MS ion peaks at m/z 358.1 [M + H]-, 1H NMR data (chloroform-d, 500MHz) : δH 6.84 (2H, sd, J = 1.5Hz, H-2’,2”), 6.79 (2H, m, J = 8Hz, H-6’6”), 6.76 (2H, m, J = 8Hz, H-5’,5”), 4.81 (1H, d, J = 5Hz, H-6), 4.37 (1H, d, J = 7Hz, H-2), 4.07 (1H, d, J = 9.5Hz, H-4a), 3.82 (1H, m, H-8b), 3.8 (1H, m, H-4b), 3.28 (2H, m, H-5, 8a), 2.84(1H, m, H-1), 5.94 (2H, s, O-CH2-O), 5.92 (2H, s, O-CH2-O); 13C NMR data (chloroform-d, 150MHz) δc 148.13 (C-3’), 147.81 (C-4’), 147.38 (C-4”), 146.74 (C-3”), 135.29 (C-1’), 132.43 (C-1”), 119.79 (C-6’), 118.87 (C-6’), 108.34 (C-5’,5”), 106,75 (C-2’), 106.59 (C-2”), 87.84(C-2), 82.83 (C-6), 71.72 (C-4), 69.88 (C-8), 54.86 (C-1), 50.36 (C-5), 101.24 (O-CH2-O), 101.17 (O-CH2-O)
2-(1,3-Benzodioxol-5-yl)tetrahydro-4-[(4-hydroxy-3-methoxyphenyl)methyl]-3-furanmethanol (2) : Yellow syrup, [ α ] D 25 11.23 (c 0.046 CH3OH), ESI-MS ion peaks at m/z 358.1 [M + H]-, 1H NMR data (methanol-d4, 500MHz): δH 6.84 (1H, d, J = 1.5Hz, H-6’), 6.79 (1H, d, J = 2 Hz, H-6), 6.78 (1H, d, J = 1.5 Hz, H-2’), 6.77 (1H, d, J = 8 Hz, H-3’), 6.67 (1H, d, J = 8 Hz, H-3), 6.63 (1H, dd, J = 2, 8 Hz, H-2), 4.75 (1H, d, J = 6.5 Hz, H-7’), 3.97 (1H, dd, J = 6.5, 8.5 Hz, H-9b), 3.73 (1H, dd, J = 6, 8.5 Hz, H-9a), 3.82 (1H, dd, J = 7.5 Hz, H-9’b), 3.63(1H, dd, J = 6.5, 11 Hz, H-9’a), 2.71 (1H, m, H-8), 2.92 (1H, dd, J = 5, 13.5 Hz, H-7b), 2.49 (1H, dd, J = 11, 13.5 Hz, H-7a), 2.33 (1H, m, H-8’), 3.83 (3H, s, OCH3-5), 5.9 (2H, d, O-CH2-O); 13C NMR data (methanol-d4, 150MHz): δc 149.38 (C-5’), 149.13 (C-5), 148.51 (C-4’), 145.96 (C-4), 138.64 (C-1’), 133.61 (C-1), 122.28 (C-2), 120.52 (C-2’), 116.63 (C-3), 113.53 (C-6), 108.97 (C-3’), 107.4 (C-6’), 84.13 (C-7’), 73.75 (C-9), 60.57 (C-9’), 54.35 (C-8’), 44.01 (C-8), 33.75 (C-7), 56.52 (OCH3-5), 102.44 (O-CH2-O)
Syringaresinol (3), Light brown syrup, ESI-MS ion peaks at m/z 418.0 [M + H]-, 1H NMR data (DMSO-d6, 500MHz): δH 6.6 (4H, s, H-2’,2”,6’,6”), 4.61 (2H, d, J = 3.5 Hz, H-2,6), 4.16 (2H, dd, J = 7, 8 Hz, H-4,8), 3.78 (2H, dd, J = 3.5 Hz, H-4,8), 3.05 (2H, m, H-1,5), 3.75 (12H, s, OCH3-3’,3”,5’,5”), 8.22 (2H, s, OH-4’,4”); 13C NMR data (DMSO-d6, 150MHz): δc 147.84 (C-5’,5”,3’,3”), 134.81 (C-4’,4”), 131.38 (C-1’,1”), 103.62 (C-2’,2”,6’,6”), 85.27 (C-2,6), 71.02 (C-4,8), 53.61 (C-1,5), 55.99 (OCH3-5’,5”,3’,3”)
(7′S,8′R,8R)-lyoniresinol-9-O-(E)-feruloyl ester (4) light brown solid, [ α ] D 25 3.00 (c 0.016 CH3OH), LC-MS ion peaks at m/z 595 [M + H]-, 1H NMR data (chloroform-d, 500MHz): δH 7.56 (1H, d, J = 15Hz, H-7”), 7.04 (1H, dd, J = 8, 2Hz, H-6”), 7.01 (1H, sd, J = 2 Hz, H-2”), 6.9 (1H, d, J = 8 Hz, H-5”), 6.48 (1H, s, H-2), 6.33 (2H, s, H-2, 6), 6.21 (1H, d, J = 15 Hz, H-8”), 4.35 (1H, d, J = 5, 11 Hz, H-7’), 4.29 (1H, dd, J = 5 Hz, 11, H-9a), 4.14 (1H, dd, J = 6, 11 Hz, H-9b), 3.6 (2H, m, H-9’), 2.68 (2H, m, J = 4.5, 15 Hz, H-7), 2.03 (1H, m, H-8’), 1.96 (1H, m, H-8), 3.92 (3”-OCH3), 3.87 (3-OCH3), 3.77 (3’,5’-OCH3), 3.39 (5-OCH3), 5.83 (4”-OH), 5.33 (4’-OH), 5.31 (4-OH); 13C NMR data chloroform-d, 150MHz): δc 167.55 (C=O), 148.38 (C-4”), 147.03 (C-3’,5’), 146.99 (C-3’,5’), 146.42 (C-4), 145.86 (C-5), 145.52 (C-7”), 137.94 (C-1’), 137.31 (C-4), 133.11 (C-4’), 128.62 (C-6), 127.04 (C-1”), 125.12 (C-1), 123.44 (C-6”), 115.25 (C-8”), 114.93 (C-5”), 109.58 (C-2”), 106.25 (C-2), 105.35 (C-2,6), 67.71 (C-9), 63.82 (C-9’), 47.83 (C-8,8’), 41.7 (C-7’), 60.17 (5-OCH3). 56.66 (3’,5’-OCH3), 56.35 (3”-OCH3), 56.25 (3-OCH3),
(-)-(2R,3R)-1-O-feruloyl-8,8’bisdihydrosiringenin (5) Brown syrup, [ α ] D 25 1.55 (c 0.024 CH3OH), ESI-MS ion peaks at m/z 597.0 [M + H]-, 1H NMR data (DMSO-d6, 600MHz) : δH 7.49 (1H, dd, J = 16Hz, H-7”), 7.23 (1H, s, H-2”), 7.05 (1H, d, J = 9Hz, H-6”), 6.72 (1H, d, J = 8Hz, H-5”), 6.39 (1H, d, J = 16Hz, H-8”), 6.35(2H, s, overlap, H-2,6,2’,6’), 6.34 (2H, s, overlap, H-2,6,2’,6’), 4.26 (1H, dd, J = 5,9Hz, H-9a), 4 (1H, dd, J = 5.5,9Hz, H-9b), 3.51 (1H, dd, J = 5.5,9Hz, H-9’a), 3.4 (1H, dd, J = 5.5,9Hz, H-9’b), 2.7 (1H, dd, J = 5.5,11.5Hz, H-7a), 2.58 (1H, dd, J = 5.5,11Hz, H-7’a), 2.5 (2H, m, overlap, H-7b, 7’b), 2.2 (1H, m, H-8), 3.79 (3”- OCH3), 3.67 (6H, 3,5,3’,5’- OCH3), 3.66 (6H, 3,5,3’,5’- OCH3); 13C NMR data (DMSO-d6, 150MHz) : δC 166.76 (C-9”), 150.59 (C-4”), 148.23 (C-3”), 147.71 (C-3,5,3’,5’), 147.66 (C-3,5,3’,5’), 145.04 (C-7”), 133.55 (C-4,4’), 133.38 (C-4,4’), 131.04 (C-1’), 130.37 (C-1), 124.49 (C-1”), 123.52 (C-6”), 115.56 (C-5”), 113 (C-8”), 110.95 (C-2”), 106.23 (C-2,6,2’,6’), 64.45 (C-9’), 64.05 (C-9), 42.7 (C-8’), 38.51 (C-8), 34.34 (C-7), 34.1 (C-7’), 55.8 (3,5,3',5',-OCH3), 55.75 (3,5,3',5',-OCH3), 55.59 (3”-OCH3)
(-)-Lyoniresinol (6) yellow syrup, [ α ] D 25 -7.4 (c 0.024 CH3OH), ESI-MS ion peaks at m/z 419.1 [M + H]-, 1H NMR data (methanol-d4, 600MHz) : δH 6.6 (1H, s, H-6), 6.4 (2H, s, H-2’,6’), 4.31 (1H, d, J = 6Hz, H-7’), 3.6 (1H, dd, J = 4.8, 10.8Hz, H-9a), 3.5 (2H, m, overlap, H-9b, 9’), 2.7 (1H, dd, J = 4.8, 15Hz, H-7a), 2.57 (1H, dd, J = 11.4, 15Hz, H-7b), 1.98 (1H, m, H-8’), 1.65 (1H, m, H-8); 13C NMR data (methanol-d4, 150MHz) : δC 149.09 (C-3’,5’), 148.77 (C-5), 147.8 (C-3), 139.93 (C-1’), 139.01 (C-4), 134.63 (C-4’), 130.29 (C-2),126.37 (C-1), 107.88 (C-6), 106.97 (C-2’,6’), 66.91 (C-9), 64.29 (C-9’), 49.18 (C-8’), 42.45 (C-7’), 41.03 (C-8), 33.72 (C-7), 60.29 (3-OCH3), 56.89 (3’, 5’-OCH3), 56.73 (5-OCH3)
Schizandriside (7) brown solid, [ α ] D 25 12.8 (c 0.001 CH3OH), ESI-MS ion peaks at m/z 491.0[M + H]-, 1H NMR data (DMSO-d6, 500MHz): δH 6.78 (1H, sd, J = 1.8 Hz, H-2), 6.68 (1H, d, J = 7.2 Hz, H-5), 6.6 (1H, s, H-2’), 6.48 (1H, dd, J = 1.8, 7.8 Hz, H-6), 6.07 (1H, s, H-5’), 4.01 (2H, sd, J = 11.2 Hz, H-7), 3.91 (1H, d, J = 7.5 Hz, H-1”), 3.83(1H, dd, J = 1.8, 9.6 Hz, H-9b), 2.98 (1H, m, H-9a), 3.65 (1H, dd, J = 5.4, 11.4 Hz, H-5”b), 2.96 (1H, m, H-5”a) 3.57 (1H, m, H-9’b), 3.47 (1H, m, H-9’a), 3.27 (1H, overlap, H-4”), 3.08 (1H, t, J = 8.4 Hz, H-3”), 2.98 (1H, m, H-2”), 2.71 (2H, d, J = 8.1 Hz H-7’), 1.87 (1H, m, H-8’), 1.7 (1H, t, J = 10.2 Hz, H-8), 3.72 (3-OCH3), 3.7 (3’-OCH3); 13C NMR data DMSO-d6, 150MHz): δC 147.08 (C-3), 145.43 (C-3’), 144.43 (C-4), 143.99 (C-4’), 136.79 (C-1), 132.58 (C-1’), 126.97 (C-6’), 121.03 (C-6), 116.21 (C-5’), 115.4 (C-5), 113.86 (C-2), 111.81 (C-2’), 104.46 (C-1”), 76.53 (C-3”), 73.3 (C-2”), 69.53 (C-4”), 67.26 (C-9), 65.62 (C-5”), 62.62 (C-9’), 45.8 (C-7), 44.86 (C-8), 37.98 (C-8’), 31.75 (C-7’), 55.57 (C-3), 55.49 (C-3)

2.3. Cell culture

Human hepatoma Hep3B (HB-8064) cell line was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Hep3B cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (Gibco-BRL, Cat. No. 16000-044), 50 U/mL penicillin and 100 mg/mL hygromycin (InvivoGen, Cat. No. ant-hg-1). The cells were maintained under standard cell culture conditions in an atmosphere of 5% CO2 at 37℃.

2.4. Cell viability

The MTT (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide) assay was performed to assess of cell viability. Hep3B Cells (3 × 10⁴cells/well) were seed in a 96-well flat-bottom microplate and incubated for 24 hours at 37℃ in a CO2 incubator. After treatment with the extracts, fractions and compounds for 24 hours, MTT solution (0.5mg/ml) was added to each well, and incubated for 3 hours. Then the supernatant was removed, and the formed formazan crystals were dissolved by adding 200µL of dimethyl sulfoxide (DMSO) per well for 30 min at 37℃ in a CO2 incubator. The absorbance measurement of each well was read at 540 nm using a microplate reader (Varioskan LUX, Thermo Fisher Scientific Inc., Waltham, MA, USA).

2.5. IL-6 induced STAT3 luciferase reporter assay.

Hep3B cells (3 × 10⁴per well) stably transformed with pSTAT3-Luc were seed into 96-well culture plates and stabilized for 24 hours. Then the medium was replaced with serum-free medium and incubated for 12 hours. The cells were treated with extracts or compounds for 1 hour followed by stimulation with 10ng/ml IL-6. After incubation for 12 hours, the medium was removed, and Passive Lysis Buffer (Promega Corp., Madison, WI, USA) was added at 60µL/well. Then lysed for 30 min, the cells lysates (25µL) were transferred to the wells (White 96-well plate). The luciferase activity was evaluated according to the manufacturer’s instructions (Promega Corp., Madison, WI, USA). The inhibitory activity with half-maximal inhibitory concentration (IC50) values (means ± S.D.) were calculated from dose-response curves of six concentrations of each compound versus normalized luciferase activity in three independent experiments (n=3) and this value was used as a measure of the inhibitory activity. Human IL-6 and static IL-6, used for the control group, were obtained from R&D System (Minneapolism MN, USA) and Sigma-Aldrich Ltd. (St. Louis, MO, USA), respectively.

2.5.1. Real-time PCR

Real-time PCR was performed according to previously reported methods [Jang et al., 2019]. Briefly, the total cellular RNA was extracted from the Hep3B cells (1 × 106 cells/well in a 6-well plate) using a Pure Link RNA Mini Kit (Invitrogen) following the manufacturer’s protocol. The complementary DNA (cDNA) was synthesized from 1 µg of the total RNA using a Superscript III First-Strand Synthesis Super Mix for qRT-PCR (Invitrogen). Quantitative real-time PCR of CRP (Hs04183452_g1), IL-1β (Hs01555410_ma), and SOCS3 (Hs02330328_s1) was performed with a TaqMan Gene Expression Assay Kit (Applied Biosystems). To normalize the gene expression, and 185 rRNA endogenous control (Applied Biosystems) was used. Quantitative real-time PCR was employed to verify the mRNA expression using a Step-One Plus Real-Time PCR System (Applied Biosystems). cDNA (1 µL), 0.5 µL of the TaqMan Gene Expression Master Mix, 0.2 µL of 18S rRNA endogenous control and 3.3 µL of dH2O were combined to give reaction mixtures with final volumes of 10 µL in each reaction tube. The amplification conditions were as follows: 10s at 95℃, 60 cycles of 5s at 95℃ and 30s at 60℃, 15s at 95℃, 30s at 60℃, and 15s at 95℃.

2.5.2. Western blot analysis

U266 cells were stimulated with IL-6 (10 ng/mL) for 20 min in the presence or absence of compound. Western blot analysis was conducted to assess STAT3 and JAK2 protein expression in the U266 cell line, as reported study [Lee et al., 2016]. The phosphorylation status of JAK2, STAT3, and ERK was examined using anti-phospho-STAT3 (1:1000), anti-STAT3 (1:1000), anti-phospho-JAK2 (1:1000), anti-JAK2 (1:1000), anti-phospho-ERK (1:1000), and anti-ERK (1:1000) antibodies (Cell Signaling, Beverly, MA, USA) and then were incubated with the appropriate horseradish peroxide-conjugated secondary antibody (1:3000) at RT, triplicate washes were followed with TBS-T, and developed for visualization using an ECL detection kit by Luminescent Image Analyzer, LAS-3000 (Fuji, Tokyo, Japan).

2.6. Statistical Analysis

Statistical analyses were performed on data collected in triplicate for all the experiments. All quantitative results are presented as means ± standard deviations (SD). Statistical analyses were performed using Prism 5 software (GraphPad Software, San Diego, CA, USA), and statistical significance was determined by one-way ANOVA followed by Dunnett’s test.

3. Results and Discussion

3.1. Identification of compounds from Lindera obtusiloba

Seven compounds were isolated from the 50% EtOH extract of L. obtusiloba Blume. The EA fractions are composed of seven lignans (1-7). Structure determination was performed using spectroscopic data, including 1H NMR, 13C NMR, 1H-1H COSY, HMBC, HMQC, HRESIMS, and polarimetry. Comparing the spectroscopic data of the isolated compounds to published literature reports, the known compounds were identified as episesamin (1) [11], 2-(1,3-Benzodioxol-5-yl)tetrahydro-4-[(4-hydroxy-3-methoxyphenyl)methyl]-3-furanmethanol (3) [21], (+)-syringaresinol (3) [4], (7′S,8′R,8R)-lyoniresinol-9-O-(E)-feruloyl ester (4) [23], (-)-(2R,3R)-1-O-feruloyl-8,8′bisdihydrosiringenin (5) [24], (-)-Lyoniresinol (6) [23] and Schizandriside (7) [25].
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Figure 1. Structure of isolated compounds 1-7 of L. obtusiloba.
Figure 1. Structure of isolated compounds 1-7 of L. obtusiloba.
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3.2. L. Obtusiloba Blume extract and fractions inhibit IL-6-induced pSTAT3 luciferase activity.

To identify IL-6/STAT3 inhibitors, IL-6-induced STAT3 activity was confirmed by luciferase assay using Hep3B cells stably expressing pSTAT3-luc. The cells were stimulated with IL-6 (10 ng/ml) for 12 h in the presence or absence of the EtOH extract or the fourteen fractions (n-hexane, chloroform, ethyl acetate, butanol, water, EA fraction 1∼10). Treatment of Hep3B cells with IL-6 alone for 12 h led to an approximately 17-fold increase in pSTAT3-Luc activity, an increase that was substantially and dose-dependently inhibited by pre-treatment with the extract and fractions for 1 h. The EA-6 fraction was found to be the most effective for inhibiting IL-6-induced pSTAT3-Luc activation, followed by the EA-7 fraction and hexane layer. The MTT assay was employed to evaluate cytotoxicity at the tested concentrations. The EA7 and 10 µg/ml EA6 fractions were found to be toxic, while all other concentrations were found to be cytotoxic. Except for the 100 µg/ml EA concentration, hexane, EA7 and the rest of the EA6 concentrations exhibited over 80% cell survival, suggesting that the inhibitory effects of these substances on IL-6-induced STAT3 activation were not related to cytotoxicity.
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Figure 2. IL-6 induced STAT3 inhibitory effect of L.obtusiloba extract and fractions were evaluated using a luciferase assay in Hep3B cells (1). The viability of Hep3B cells after treatment with L.obtusiloba extract and fractions. 1: 50% EtOH extract, 2: Hexane fraction, 3: Ethyl acetate, 4: Butanol, 5: water, 6 - 15: EA fraction 1 – 10.
Figure 2. IL-6 induced STAT3 inhibitory effect of L.obtusiloba extract and fractions were evaluated using a luciferase assay in Hep3B cells (1). The viability of Hep3B cells after treatment with L.obtusiloba extract and fractions. 1: 50% EtOH extract, 2: Hexane fraction, 3: Ethyl acetate, 4: Butanol, 5: water, 6 - 15: EA fraction 1 – 10.
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3.3. Inhibitory effects of isolated compounds on IL-6 induced pSTAT3 luciferase activity

All isolated compounds were measured for their inhibitory effect on IL-6-induced pSTAT3 luciferase activity in Hep3B cells. Additionally, an MTT assay was employed to evaluate cytotoxicity at the tested concentrations (data not shown), and the results indicated that the observed bioactivities were not due to cellular cytotoxic effects. Among the tested compounds, compound 5 showed the most significant inhibitory effects with an IC50 value of 10.83 µM, which was compared with stattic (a positive control with an IC50 value of 0.27 µM). Additionally, compound 1 exhibited high inhibitory effects with an IC50 value of 14.3 µM, and compound 4 exhibited a slight inhibitory effect with an IC50 value of 40.39 µM. Considering these results with respect to preliminary structural requirements for activity, two partial structures responsible for the bioactivity cold be suggested as follows: a feruloyl moiety and a 1,4-diphenylbutane moiety. Based on these bioassay results, we hypothesized that compound 1 and 5 could be promising IL-6/STAT3 inhibitors.
Table 1. Inhibitory effect of compounds 1-7 on IL-6/STAT3 activation.
Table 1. Inhibitory effect of compounds 1-7 on IL-6/STAT3 activation.
compounds IC50(µM)
1 14.3±2.67
2 >50
3 >50
4 40.39±3.71
5 10.83±2.40
6 >50
7 >50
stattica 0.27±0.03
Data are expressed as the IC50 values of three independent experiments (n=3). a Stattic was used as the positive control.

3.4. Inhibitory effects of active compounds on IL-6 induced gene expressions

Next, we investigated whether the IL-6-induced pSTAT3 luciferase inhibitory effects of compounds affect pSTAT3 induced gene expressions. To confirm that, gene expressions of CRP, IL-1β and SOCS3 were analyzed using quantitative real time PCR. The gene expressions were significantly downregulated 30 μM of compound 1 and 30 and 60 μM of compound 5 treatment (Figure 3).

3.5. Effect of compound 1 and 5 on IL-6/STAT3 signaling molecules

To determine inhibitory mechanism of pSTAT3-induced gene, pSTAT3 expressions of Hep3B cells were investigated using immunofluorescence staining. The results showed that the compounds downregulated pSTAT3 nuclear translocation and pSTAT3 expression (Figure 4). Furthermore, western blot analysis of JAK2 and STAT3, which are IL-6 signaling molecules, the compound treatment decreased phosphorylations of JAK2 and STAT3 (Figure 5). These results indicate that the compounds regulate IL-6/STAT3 signaling through JAK2.

4. Conclusions

The stems of Lindera obtusiloba Blume are known to have inhibitory effects on various inflammatory responses such as neuroprotective activity and anti-allergic activity [13,15]. Based on this, compounds with potential as a promising inflammatory response inhibitor from the stems of Lindera obtusiloba have been reported. Previous physiochemical studies have represented that is consists of secondary metabolites such as lignan, neolignan, flavonoids, and butanolide [3,4,5]. In particular, compounds of the lignan family, such as xanthoxyol, syringaresinol, and linderin A, have been reported to have inhibitory activity against various inflammatory diseases [15]. In this study, compounds with IL-6-induced STAT3 inhibitory activities from the stems of Lindera obtusiloba Blume are investigated. There are seven types of compounds isolated from the 50% ethanol extract of Lindera obtusiloba Blume stem, and the structures of isolated compounds were identified using NMR and MS. The isolated compounds were mainly compounds of lignan family. These have structural features linked through C8-C8’ linkage. Most lignans represent physiologically important functions for plant defense and human health. Lignans are grouped according to functional groups such as furofuran, tetrahydrofurans, dibenzylbutanes and arylnaphthalenes [26,27].
IL-6 is a major cytokine involved in the inflammatory response and is a key upstream mediator of STAT3 [17]. IL-6 signaling is provoked by binding IL-6 to IL-6 receptor (IL-6R) complexes, which are related with IL-6, IL-6Ra, and gp130 receptor chains, and its activation result in the JAK2/STAT3 and MAPK signaling pathways [18,19]. The IL-6-induced JAK2/STAT3 signaling pathway plays a positive role in inflammation and neoplasia. The regulation of IL-6 is a useful method to inhibit the inflammatory response by regulating the phosphorylation of JAK2 and STAT3. All the isolated compounds (1-7) were evaluated for their IL-6-induced STAT3 inhibitory effects in Hep3B cells using a luciferase reporter assay. Additionally, we investigated whether compounds could inhibit the phosphorylation of STAT3 and JAK2.
In conclusion, the isolated compounds are identified for lignan family having furofuran and naphthalene functional groups. All the isolated compounds (1-7) were evaluated for their IL-6-induced STAT3 inhibitory effects in Hep3B cells using a luciferase reporter assay. Of the isolates, compounds 1 and 5 showed strong inhibitory effects on IL-6-stimulated STAT3 activation. These results can provide valuable biochemical information for the use of L. obtusiloba as a pharmaceutical material, and the bioactive compounds obtained from this plant may be promising candidates for the treatment of IL-6-mediated STAT3 disease. Further studies will be needed to elucidate the precise mechanism of these materials on the up- or downstream IL-6 signaling pathways, and their therapeutic efficacies as potent IL-6 inhibitors will be investigated in vivo, such as in arthritis or ovariectomized animal models.

Acknowledgments

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Crop Viruses and Pests Response Industry Technology Development Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA)(120087-5) and a grant from the KRIBB Research Initiative Program (KGM5242113).

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Figure 3. Effects of compounds 1 and 5 on the mRNA expression of IL-6-induced gene expressions. Hep3B cells were pretreated with 1 and 5 at 30 and 60 μM for 1 h and induced with IL-6 (10 ng/mL) for 5 h. The gene expressions were analyzed using real-time PCR. C, 20 μM of cirsiliol. *p<0.05, **p<0.01 compared with the only IL-6 treated group. Values are expressed as the means ± S.D. of three individual experiments. * p < 0.05 and ** p < 0.01 versus the only- IL-6-treated control group obtained through one-way ANOVA followed by Dunnett’s test.
Figure 3. Effects of compounds 1 and 5 on the mRNA expression of IL-6-induced gene expressions. Hep3B cells were pretreated with 1 and 5 at 30 and 60 μM for 1 h and induced with IL-6 (10 ng/mL) for 5 h. The gene expressions were analyzed using real-time PCR. C, 20 μM of cirsiliol. *p<0.05, **p<0.01 compared with the only IL-6 treated group. Values are expressed as the means ± S.D. of three individual experiments. * p < 0.05 and ** p < 0.01 versus the only- IL-6-treated control group obtained through one-way ANOVA followed by Dunnett’s test.
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Figure 4. Effects of compound 1 and 5 on IL-6-induced p-STAT3 expression in Hep3B cells. Representative green fluorescence microscopy images indicating pSTAT3 stained by Alexa 488 and blue fluorescence microscopy images indicating the nucleus stained by DAPI. The Hep3B cells were treated with compounds 1 and 5 for 1 h and stimulated with 10 ng/ml IL-6 for 30 min. C20, 20 μM of cirsiliol.
Figure 4. Effects of compound 1 and 5 on IL-6-induced p-STAT3 expression in Hep3B cells. Representative green fluorescence microscopy images indicating pSTAT3 stained by Alexa 488 and blue fluorescence microscopy images indicating the nucleus stained by DAPI. The Hep3B cells were treated with compounds 1 and 5 for 1 h and stimulated with 10 ng/ml IL-6 for 30 min. C20, 20 μM of cirsiliol.
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Figure 5. Effects of compounds 1 and 5 on the IL-6-induced phosphorylation of JAK2 and STAT3 in U266 cells. Cells were pretreated with compound 1 and 5 at 30 and 60 μM for 1 h and treated with IL-6 (10 ng/ml) for 20 min. The p-STAT3 and p-JAK2 proteins were detected by Western blot analysis. The total nonphosphorylated proteins served as a loading control for the phosphorylated protein.
Figure 5. Effects of compounds 1 and 5 on the IL-6-induced phosphorylation of JAK2 and STAT3 in U266 cells. Cells were pretreated with compound 1 and 5 at 30 and 60 μM for 1 h and treated with IL-6 (10 ng/ml) for 20 min. The p-STAT3 and p-JAK2 proteins were detected by Western blot analysis. The total nonphosphorylated proteins served as a loading control for the phosphorylated protein.
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