PreprintArticleVersion 2Preserved in Portico This version is not peer-reviewed
Flow-cytometry Assessment of DNA content Analysis and Immunophenotyping of Immune-cells in Lymph-node-specimens as Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas
Version 1
: Received: 14 November 2023 / Approved: 14 November 2023 / Online: 15 November 2023 (01:56:10 CET)
Version 2
: Received: 31 December 2023 / Approved: 3 January 2024 / Online: 3 January 2024 (05:31:53 CET)
Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Chepa, S.R.; Eshel, E.; Zarfati, M.; Stemer, G.; et al. Flow-Cytometry Assessment of DNA Content and Immunophenotyping of Immune-Cells in Lymph-Node-Specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Annals of Hematology 2024, doi:10.1007/s00277-024-05807-8.
Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Chepa, S.R.; Eshel, E.; Zarfati, M.; Stemer, G.; et al. Flow-Cytometry Assessment of DNA Content and Immunophenotyping of Immune-Cells in Lymph-Node-Specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Annals of Hematology 2024, doi:10.1007/s00277-024-05807-8.
Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Chepa, S.R.; Eshel, E.; Zarfati, M.; Stemer, G.; et al. Flow-Cytometry Assessment of DNA Content and Immunophenotyping of Immune-Cells in Lymph-Node-Specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Annals of Hematology 2024, doi:10.1007/s00277-024-05807-8.
Azoulay, D.; Tapuchi, T.; Ronen, O.; Akria, L.; Cohen, H.I.; Surio, C.; Chepa, S.R.; Eshel, E.; Zarfati, M.; Stemer, G.; et al. Flow-Cytometry Assessment of DNA Content and Immunophenotyping of Immune-Cells in Lymph-Node-Specimens as a Potential Diagnostic Signature of Aggressiveness in B-Non-Hodgkin Lymphomas. Annals of Hematology 2024, doi:10.1007/s00277-024-05807-8.
Abstract
Introduction: Flow-cytometry (FC) is a powerful tool that can assist in lymphoma diagnosis in lymph node (LN) specimens. Although lymphoma diagnosis and classification are mainly based on tumor cell characteristics, surrounding cells are less employed in this process.Methods: We retrospectively investigated alterations in the ploidy status, proliferative cell fraction (PF) and the percentages of surrounding immune cells in 62 consecutive LN specimens with B-Cell Non-Hodgkin Lymphoma (B-NHL) that were submitted for FC evaluation between 2019-2022.Results: Compared with indolent B-NHLs, aggressive B-NHLs show increased DNA aneuploidy and PF, increased monocytes, immature-granulocytes, mature granulocytes, CD8+ T-cells, Double-Negative-T-cells and Double-Positive-T-cells, and decreased total CD45+ cells, total lymphocytes, CD4+ T-cells and CD4/CD8 ratio. Receiver operating characteristic analysis determined PF > 6.8% and immature-granulocytes > 0.9% as optimal cutoffs with highest specificity and sensitivity in differentiating aggressive and indolent B-NHLs.Conclusions: These findings further strength the diagnostic value of DNA content analysis by FC and suggest the utilization of tumor surrounding immune cells in NHL diagnosis and classification.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
3 January 2024
Commenter:
David Azoulay
Commenter's Conflict of Interests:
Author
Comment:
1. We removed the data of reactive specimens to focus in indolent vs. aggressive specimens. 2.We improved the figure 1 and figure 2 by adding more details and removing information regarding non hematopoietic cells from the illustration. 3. We added an author. 4. We added more references that previously show similar data.
Commenter: David Azoulay
Commenter's Conflict of Interests: Author