Prerpints.org logo
Preprint
Review

The Effects of Intermittent Cold Exposure on Adipose Tissue

Altmetrics

Downloads

117

Views

40

Comments

0

A peer-reviewed article of this preprint also exists.

This version is not peer-reviewed

Submitted:

15 November 2023

Posted:

16 November 2023

You are already at the latest version

Alerts
Abstract
Intermittent cold exposure has garnered increased attention in popular culture, largely for its proposed effects on mood and immune function, but there are also suggestions that the energy wasting mechanisms associated with thermogenesis may decrease body weight and fat mass. Considering the continued and worsening prevalence of obesity and type II diabetes, any protocol that can reduce body weight and/or improve metabolic health would be a substantial boon. Here, we present a narrative review exploring the research related to ICE and adipose tissue. Any publicly available original research examining the effects of repeated bouts of ICE with adipose related outcomes was included. While ICE does not consistently lower bodyweight or fat mass, there does seem to be evidence for ICE as a positive modulator of the metabolic consequences of obesity, such as glucose tolerance and insulin signaling. Further, ICE consistently increases the activity of brown adipose tissue (BAT) and transitions white adipose tissue to a phenotype more in line with BAT. Lastly, the combined effects of ICE and exercise do not seem to provide any additional benefit, at least when exercise is done during ICE bouts. The majority of the current literature on ICE is based in rodent models where animals are housed in cold rooms, which does not reflect protocols likely to be implemented in humans such as cold-water immersion. Future research could specifically characterize ICE via cold water immersion in combination with controlled calorie intake to clearly determine the effects of ICE as it would be implemented in humans looking to lower bodyweight via reductions in fat mass.
Keywords: 
Subject: Biology and Life Sciences  -   Endocrinology and Metabolism

1. Introduction

Obesity and type 2 diabetes constitute a global epidemic. Dysfunction of adipose tissue has been implicated in the pathophysiology of metabolic syndrome (MetS) [1], which is a cluster of symptoms including abdominal obesity, impaired blood glucose homeostasis, hypertension, and dyslipidemia [2]. MetS is an underlying factor in heart disease, stroke [3], and cognitive impairments such as Alzheimer’s disease and dementia [4]. Properly functioning adipose tissue is indispensable in the maintenance of metabolic health, as has been established in numerous studies in animals and humans [5].
Given the irreplaceable role of healthy adipose tissue in maintaining proper metabolic status, intense investigation has focused on enhancing the function of adipose tissue in several contexts. As an example, one class of drugs, the thiazolidinediones (TZDs), have demonstrated evidence of improving glucose homeostasis through its action on adipocytes via induction of PPARγ, the master transcriptional regulator of adipocyte function [6]. However, TZDs exhibit undesirable side effects including weight gain, cardiac complications, and increased bone fractures [7]; therefore, they are generally contraindicated. In view of the paucity of favorable outcomes in treating obesity and adipose tissue dysfunction with pharmacological approaches, strategies utilizing lifestyle modification such as dietary and exercise interventions have been explored as logically understandable methods for promoting weight loss, maintenance, and combating metabolic abnormalities. However, despite decades of admonitions to reduce caloric intake and increase energy expenditure using these traditional approaches, the obesity epidemic has proven to be recalcitrant and has only intensified in recent years [8].
Recently, the practice of intermittent cold exposure (ICE) has received attention in popular culture as a modality for improving mood, increasing immune function, and reducing inflammation with some scientific evidence backing those anecdotal reports [9,10,11,12]. ICE has also been suggested as a potential modulator of body weight and adipose tissue function. Cold exposure challenges maintenance of thermal homeostasis, to which homeothermic animals must mount an orchestrated set of defensive responses. These include sympathetic nervous system activation, shivering, and non-shivering thermogenesis [13]. The activation of brown adipose tissue (BAT), which specializes in high rates of substrate metabolism, thereby generating heat as a by-product, is a critical mechanism by which core body temperature is maintained in response to cold exposure [14]. Given the capacity of BAT to metabolize substrate at high rates per unit of tissue mass, it is logical to hypothesize that activation, and perhaps expansion, of BAT might constitute a modality to combat obesity by increasing energy expenditure (EE). Indeed, support for this notion is available in the literature [15,16]. Perhaps more exciting is the speculation that white adipose tissue (WAT), which generally does not exhibit high rates of substrate oxidation, might undergo a shift towards a phenotype more consistent with BAT, a phenomenon referred to as “beiging”. By activating BAT and altering the metabolic function of WAT, cold exposure might exert specific effects on adipose tissue that could act therapeutically in the treatment of obesity and its associated pathologies [17,18].
This narrative review aims to summarize the current state of the field pertaining to intermittent cold exposure as an intervention to combat obesity and obesity induced metabolic syndrome. Emphasis will be placed on the specific effects of repeated bouts of ICE on adipose tissue. The review will discuss mechanisms by which ICE could alter the metabolic and endocrine function of adipose tissue in a manner consistent with increased resistance to obesity, thereby attenuating the disease burden stemming from the dysfunction of adipose tissue, which constitutes a hallmark characteristic of the metabolic syndrome.

2. Overview of Included Literature

We have included all available literature that examined the effects of intermittent cold exposure (ICE) on adipose tissue or, at the very least, body weight. The methods varied and are described both here and in Table 1 with specific detail given to ICE protocols, although many of the studies had broader interests which are partially described in the group columns of Table 1. In all, we have 20 studies that have spanned from 1979 to 2023; although, the data presented in Deshaies et al. is essentially an addendum to Arnold et al., where both studies are reporting outcomes from the same animal cohort [19,20]. The majority of included literature examined the effects of ICE on rodents with a fairly even mix of rat and mouse models, although literature examining the effects of ICE on adipose tissue in humans are also present. There was quite a bit of variability in ICE protocols with respect to ICE duration (<5min-8 hours) and in the span of intervention (2 weeks - 6 months). Further, rodent studies exclusively used cold rooms (air) while studies in humans had a combination of water-cooled suits, cold showers, and cold-water immersion. Cold showers and cold-water immersion would be the most likely method for implementation for people looking to adopt an ICE protocol, but the majority of the literature, again, is rodents housed in cold rooms. ICE frequency in our included studies was fairly consistent; most studies examined ICE at 5 days per week or daily. Lastly, most studies examine ICE intensity at 4-10°C, but some utilized temperatures as low as -20°C for a shorter duration.

3. ICE Effects on Bodyweight, Energy Expenditure, and Adipose Tissue

The recent increased interest in intermittent cold exposure (ICE) is related to the proposed benefits for mood, inflammation, immune function, and general physical wellness. While not as commonly mentioned, there are suggestions that ICE could reduce bodyweight as a function of increased energy expenditure (EE) due to shivering (ST) and non-shivering thermogenesis (NST). ST and NST are considered the major energy sink in the context of cold exposure [37,38], but there is the potential for other factors related to ICE that may modulate energy homeostasis, such as hormone modulation [39,40], that are not discussed in this section. Here and in Table 1, we outline the effects of ICE on bodyweight (BW), adipose tissue weight (white adipose tissue, WAT, and brown adipose tissue ,BAT), adipose tissue morphology (WAT and BAT), EE, and outcomes that may be related to EE.
Table 2. ICE effects on bodyweight, adipose tissue weight/morphology, and energy balance.
Table 2. ICE effects on bodyweight, adipose tissue weight/morphology, and energy balance.
Year First
Author		 Citation ICE Outcomes Model
1979 Doi [21] ↑BW (adults), ↔BW (newborns), ↑BAT, ↑EE in response to NorEp, ↑NST, ↓ST Rats
1984 Harri [22] ↓Weight gain, ↔ BW (trended ↓), ↑BAT, ↑NST, ↓ST Rats
1986 Arnold [19] ↓BW, ↓FFM, ↓FM, ↑BAT, ↑EE, ↑EI Rats
1988 Deshaies [20] ↓BW, ↓eWAT and %eWAT, ↑BAT and %BAT Rats
1989 Yahata [23] ↓BW, ↓eWAT, ↓BAT, ↑EI Rats
2014 Yoo [24] ↑BW, ↔FFM, ↑FM, ↑iWAT, ↑iWAT adipocyte size, ↑iWAT beiging, ↑eWAT, ↓eWAT adipocyte size, ↑BAT activation, ↔EE Mice
2014 Qiao [25] Adipoq-/- : ↑iWAT beiging, ↓Thermogenesis Mice
2014 Ravussin [26] Cohort 1 (1 or 4hrs ICE): ↔BW, ↔FM, ↔FFM, ↔iWAT, ↔eWAT, ↔BAT, ↑EE (4hrs), ↑EI (4hrs)
Cohort 2 (4 or 8hrs ICE): ↔BW, ↔FM, ↔FFM, ↔iWAT, ↑eWAT (4&8hrs), ↔BAT, ↑EE (4hrs), ↑↑EE(8hrs), ↑EI (4hrs), ↑↑EI (8hrs)		 Mice
2014 Blondin [27] ↑BAT activity during CE, ↓Skin Temp, ↓ST Humans
2015 Wang [28] ↔BW, ↔ sWAT, ↓sWAT adipocyte size, ↓vWAT, ↑BAT activity, ↑BAT adipocyte number Mice
2015 Bai [29] ↔BW, ↔sWAT, ↓sWAT adipocyte size, ↑pericardial WAT, ↓pericardial adipocyte size, ↑pericardial WAT beiging Platue Pika (Rodent)
2016 Gibas-Dorna [30] Winter Swimmers vs Controls: ↑BW, ↑FM, ↓vWAT
Winter Swimmers (pre vs post winter swimming season): ↔BW, ↔FM, ↔vWAT		 Humans
2016 Tsibui'nikov [31] ↑BW (↑8hrs, ↑↑1.5hrs), ↑BAT weight (8hrs) Rats
2017 Blondin [32] ↔BW, ↑BAT volume, ↑BAT activity with CE, ↓skin temp, ↓ST, ↑EE with CE, ↔ fuel utilization during CE Humans
2019 Presby [33] ↔BW, ↔FM, ↔FFM, ↔iWAT, ↔eWAT, ↑sWAT beiging, ↑BAT weight, ↑BAT adipocyte size, ↑EE during CE, ↓EE during dark cycle, ↑EE during light cycle Obese Mice
Caloric Restriction During ICE
24hr adlibitum after ICE
2021 Soberg [16] Winter Swimmers: higher supraclavicular skin temp in response to cold exposure, No BAT glucose uptake at thermoneutrality (controls had glucose uptake at thermoneutrality), ↑glucose uptake in perirenal BAT during cold exposure (not significant for control), ↑REE during cold exposure Humans
2021 Zhang [34] ↓BW, ↑EI (trend) Rats
2022 McKie [35] ↑BW, ↑iWAT, ↑eWAT, ↑BAT, ↑EI, ↑↑EI (within 4hrs post ICE) Obese Mice
2023 Weng [36] ↑sWAT, ↔vWAT Obese Rats
2023 Nema [9] ↔BW, ↔BMI, ↔FM, ↔FFM, ↓Waist Circumference (men only) Humans (Soldiers)
Legend: increased (↑), no change (↔), decreased (↓), cold exposure (CE), intermittent cold exposure (ICE), body weight (BW), fat mass (FM), fat free mass (FFM), white adipose tissue (WAT), inguinal WAT (iWAT), epididymal WAT (eWAT), subcutaneous WAT (WAT), visceral WAT (vWAT), brown adipose tissue (BAT), non-shivering thermogenesis (NST), shivering thermogenesis (ST), energy expenditure (EE), energy intake (EI).
Bodyweight and Energy Expenditure. The effects of ICE on BW are mixed, with studies reporting increases [21,24,31,35], decreases [19,22,34], and null effects [9,26,28,29,32]. Still, the methodologies were varied, and a closer look provides some potential insights. Studies using human subjects generally show no change in BW [9,32] but do report a modest decrease in waist circumference [9], while rodent studies represent much of the variability initially mentioned. ICE duration was examined directly by Ravussin et al. and Tsibui’nikov et al. The former directly measured the differential effects of ICE at 1, 4, or 8hs/d and all conditions demonstrated no change in BW [26]. The latter compared 1.5hrs to 8hrs of ICE, where both groups demonstrated increased BW, and the increase in BW was more substantial with the shorter ICE duration [31]. The intensity of ICE may add additional context as Harri et al. used -20°C for 60min per ICE session and found a significant decrease in weight gain over the course of the study [22]. Arnold et al. also used a comparatively more intense ICE temperature, -5°C, where decreased BW was also reported [19]. Lastly, length of ICE interventions did not serve as an explanatory factor, where longer interventions, 10-14wks, seem to confirm a null effect [26,28] and shorter interventions cover the gamut of the initially mentioned disparity. The overall data on ICE does not provide solid evidence on modulating bodyweight in either direction but increases in intensity may be more likely to induce a reduction in BW.
Inconsistency is less dramatic as related to EE in response to ICE. Studies generally indicate increased energy expenditure [16,19,33], further increasing with increased ICE duration [26]. While EE is clearly increased in response to ICE, Presby et al. adds additional nuance, examining EE in the light and dark cycles. ICE was shown to induce increased EE during the times generally associated with sleep for rodents, but decreased EE during the active dark cycle [33]. However, Presby et al. implemented ICE 3 hours prior to the sleep cycle, which may implicate the ICE timing rather than ICE generally.
An overall null effect of ICE on BW is expected when the balance between EE and energy intake (EI) is maintained, and an expected increase in EI, as an effect of ICE, is reported by most studies [19,23,26,34,35]. Interestingly, Yoo et al. capped EI to pre-ICE intervention levels and still reported an increase in BW [24]. Unfortunately, they reported limitations in their ability to accurately measure total EE in the ICE exposed mice; instead reporting no difference in EE at thermoneutrality in both control and ICE exposed mice without account for their EE during CE. Further, Presby et al. also controlled EI and reported a null effect on BW along with an overall null effect on EE, despite increased EE during cold exposure [33]. Still, when considering the combined reports, ICE seems to increase both EE and EI when calories are available ad libitum resulting in an overall null effect on BW based on the combined literature.
White Adipose Tissue Weight and Morphology. Despite the lacking evidence for ICE to modulate BW, there is some evidence for its impact on white adipose tissue (WAT) weight and implications for WAT function based on morphology. Generally, ICE tends to increase subcutaneous WAT (sWAT) [24,30,35,36] and has variable effects on visceral WAT (vWAT), where increases [24,26,29,35], decreases [20,23,28,30], and null effects [33,36] are reported. In humans, winter swimmers have increased fat mass, but a decrease in vWAT as estimated via electrical impedance [30]. In rodents, Yoo et al. reports an increase in both sWAT and vWAT [24], a report that is not consistent across the ICE related literature. As mentioned above, Yoo et al. maintained EI to pre-ICE intervention levels, but still report a significant increase in WAT weight which accounts for most of their reported increases in BW [24]. However, they also showed that ICE induced an increase in WAT multilocularity, multiple lipid droplets within a single adipocyte. This suggests a transition to a more metabolically active beige phenotype of WAT and is indicative of increased thermogenic capacity [41]. The report of ICE-induced WAT beiging is consistent with other morphology-based reports [29,33]. Overall, reports for changes in WAT mass are too inconsistent to be conclusive, but sWAT tends to increase in response to repeated bouts of ICE. Further, studies support ICE as a protocol for inducing a beige adipocyte morphology, related to increased mitochondria and thermogenesis, which may indicate improvements in the metabolic activity of WAT in rodents and humans exposed to ICE. More support for ICE-induced WAT beiging is discussed in section 4.
Brown Adipose Tissue Weight, Morphology, and Energy Expenditure. While WAT beiging is associated with increased capacity for WAT thermogenesis, brown adipose tissue (BAT) is generally considered the prominent site for adipocyte thermogenesis per tissue weight, although WAT does comprise a substantially larger tissue mass that seems to increase with ICE. ICE tends to increase BAT weight, based on the majority of studies [22,28,31,32,33]. Although, one study indicates no change [26] and another reports a decrease [23]. While there is a small disparity in reports for ICE-induced increases in BAT weight, there are clear effects on BAT activity. ICE consistently increased the thermogenic response of BAT to a cold stress, where rodents [24] and humans [16] that undergo regular ICE show increased BAT activation in response to cold exposure. Interestingly, BAT activation is decreased at thermoneutrality in humans who participate in ICE compared to those who do not [16]. Overall, ICE increases BAT activation in response to a cold stress and likely increases BAT weight.
Overall Conclusions from Table 1. While ICE increases EE, BAT activation, and WAT beiging, there is no clear evidence for its potential to decrease FM and BW. In fact, some studies report both increased BW and FM [24,30,35], which may implicate compensatory mechanisms based on the consensus for ICE to increase EI. While BW and FM may increase, the increase seems to be predominantly sWAT rather than vWAT, where increases in vWAT would be generally associated with poor metabolic and health outcomes. Further, both sWAT and vWAT consistently adopt a beiging phenotype in response to ICE, and a transition from a primarily energy storing phenotype, to a more metabolically active tissue, could imply additional benefits. There may still be possibility for ICE induced weight loss when combined with caloric restriction, although reports from EI controlled studies suggest a substantial caloric deficit would be needed [24,33].

4. ICE Effects on Adipose Tissue Gene and Protein Expression

Adipose tissue function, both in a tissue mass and secretory/endocrine context, is modulated by alterations in gene and protein expression in response to environmental stimuli. Intermittent cold exposure (ICE) induces a potent challenge to thermal homeostasis, thereby evoking systemic responses mobilized to defend core body temperature. Adipose tissue has been demonstrated in several studies to be highly responsive to ICE. This responsiveness is not limited to brown adipose tissue (BAT), although BAT has been regarded, logically, to be a major player in regulating body temperature via its high rates of substrate turnover enabled by abundant mitochondria and the high expression of uncoupling proteins (UCPs). These UCPs enable high rates of substrate oxidation uncoupled from generating ATP, with the result of this uncoupled cellular respiration being robust heat generation and, therefore, maintenance of homeostatic body temperature in response to cold challenge. However, research interest in ICE’s effect on adipose tissue has expanded beyond the relatively well-understood role of BAT in cold tolerance to examination of how ICE could alter the patterns of gene and protein expression in white adipose tissue (WAT). Changes in adipose tissue gene and protein expression were reported by 11 of our 20 included studies. Those results are presented in Table 3 and discussed below.
Brown Adipose Tissue Gene and Protein Expression. BAT is characterized by abundant mitochondria, high expression of UCP1, and multilocular lipid droplets, underlying many of the adaptive thermogenic responses to cold exposure and enabling the defense of body temperature in euthermic animals. The cellular lineage of BAT is markedly different from WAT and exhibits similarity to skeletal muscle insofar as both cell types derive from stem cell precursors expressing the transcription factors Pax7 and Myf5 [42]. The responsiveness of BAT to cold exposure has logically received much attention due to its specialized capacity to generate heat by dissipating chemical energy in mitochondria through uncoupled cellular respiration and ATP synthesis. A line of experimental evidence has supported the overall theme that BAT responds to cold exposure by increasing tissue mass via hypertrophy and hyperplasia, induction of uncoupling protein 1 (UCP1) and PPARG coactivator 1 alpha (PGC1α) expression, and increasing fat utilization capacity via increased expression of lipoprotein lipase [26,28,33].
White Adipose Tissue Gene and Protein Expression. WAT has traditionally been regarded solely as a somewhat passive site for storage of excess caloric energy in the form of neutral lipids, thus enabling this tissue to serve as a regulator of nutrient homeostasis [43] . However, upon the discovery of adipocyte-derived factors such as leptin and adiponectin, scientific interest in adipose tissue as a bona fide endocrine organ intensified greatly [44]. WAT has been shown to be highly responsive to temperature; indeed, subcutaneous WAT is uniquely situated to provide sensory input on ambient temperature to the organism [43].
Experiments in mice examining the responsiveness of WAT, in subcutaneous and visceral fat depots, to cold exposure provide evidence that a thermogenic transcriptional program was induced in a cell autonomous manner [45]. The notion that WAT could shift towards a phenotype more related to BAT and thereby become more thermogenic is tantalizing insofar as such a shift could result in greater energy expenditure, representing a possible anti-obesity therapeutic modality. Several experiments have demonstrated that ICE results in alterations specifically in WAT gene and protein expression that underlie a thermogenic transcriptional program. Increased UCP1 expression in WAT has been demonstrated in several studies, supporting the notion that cold exposure could result in increased substrate oxidation in WAT [25,28,33], supporting the notion for ICE induced beiging of white adipose tissue.
Interestingly, beige or “brite” (brown within white) adipocytes have been shown to constitute a distinct cell type resident within WAT depots exhibiting high responsiveness to cold exposure and ready induction to a thermogenic gene expression program enabled by sensitivity to irisin, a polypeptide hormone secreted by muscle [41,46]. In this context, a mechanism by which ICE could act as an exercise mimetic can be proposed, thereby making this concept more concrete. Further support for the notion that WAT is highly plastic and sensitive to temperature was provided in experiments showing that the thermogenic capacity of beige adipocytes induced by cold exposure within WAT depots can be reversed upon removal of cold exposure for a duration of 5 weeks [47].
Overall Conclusions from Table 2. Taken together, results mainly from rodent experiments support the notion that ICE promotes the initiation of a thermogenic gene expression profile in both BAT and WAT, with a more robust induction of thermogenesis in BAT than in WAT. This is expected given that BAT is specialized, particularly in rodents, to generate heat by dissipating the normally tight coupling between substrate oxidation and ATP synthesis. Regardless, the confirmation that WAT can undergo beiging upon exposure to cold challenge implicates this tissue as potentially thermogenic factor. While evidence that cold exposure can independently result in loss of bodyweight and reduce bodyfat percentage is lacking, the shift from a primarily energy-storing to an energy-dissipating phenotype offers a rationale for considering ICE as a modality to promote metabolic health insofar as this shift in gene and protein expression could result in greater energy expenditure. In this context, alterations in adipose tissue gene and protein expression promoting thermogenesis constitute a line of evidence supporting the use of ICE as a modality to promote a beneficial metabolic phenotype that is resistant to the development of insulin resistance and the metabolic consequences of obesity.

5. ICE Effects on Systemic Factors Related to Adipose Tissue and Metabolism

Insulin, Glucose Homeostasis, and Adipokines. Metabolic syndrome (MetS) is characterized by defects in insulin action and blood glucose homeostasis stemming from hyperinsulinemia and insulin resistance in peripheral tissues [48]. Moreover, evidence from multiple studies has implicated hyperinsulinemia per se as a hallmark abnormality underlying a leading cause of mortality globally, atherosclerotic cardiovascular disease [49]. Given the role played by insulin resistance and hyperglycemia in morbidity and mortality, interventions showing potential in enhancing insulin action in peripheral tissue present opportunities to combat a range of pathologies associated with metabolic syndrome. In view of the indispensable role played by healthy adipocytes in maintaining metabolic health via their endocrine signaling and the evidence for cold exposure in favorably altering the metabolic function of adipose tissue [28,43] it is reasonable to speculate that intermittent cold exposure (ICE) could enhance insulin sensitivity and glucose homeostasis via specific effects on adipose tissue. In Table 4, we summarize the reports for ICE induced changes in these systemic factors and discuss them below.
Numerous studies have investigated the effect of cold exposure on the secretory function of adipose tissue and possible modulation of insulin sensitivity and blood glucose via changes in circulating adipokines such as adiponectin and leptin. Unfortunately, the effectiveness of ICE on inducing these systemic factors remains ambiguous, with no clear consensus emerging from the studies done to date. The evidence for ICE as a potent stimulus for increased secretion of adiponectin and leptin, which promote insulin sensitivity and satiety respectively, is somewhat limited. While Ravussin et al. observed a transient increase in glucose tolerance without direct effects on insulin in mice, there was no increase in serum adiponectin nor were there changes in body weight; these observations were largely attributed to compensatory increases in food intake in response to ICE [26]. This logically leads one to hypothesize that administration of supplementary leptin in ICE might offset compensatory increases in food intake. Unexpectedly, Mckie et al. shows a null effect of leptin injection in mice undergoing an ICE protocol but shows the expected reduction in food intake for control mice [35]. With respect to adiponectin, it is notable that adipose tissue-specific adiponectin KO mice did not exhibit the same extent of adaptation to ICE as control mice, perhaps indicating that adiponectin plays at least a permissive role in mediating adaptations to ICE, thereby underscoring the importance of adipose tissue as a mediator in response to ICE [25].
Although direct evidence of a linkage between ICE and the modulation of adipokines, such as leptin and adiponectin, is fairly limited, some studies have demonstrated favorable alterations in glucose homeostasis and insulin sensitivity associated with changes in adipose tissue biochemical factors. Weng et al. reported that a combination of ICE and exercise in rats resulted in several effects on free fatty acid (FFA) production and utilization, producing the net effect of reducing insulin resistance, HOMA-IR, and blood glucose, with a main effect of ICE independent of exercise [36]. This study further demonstrated that adipose triglyceride lipase (ATGL) and lipoprotein lipase (LPL) activity in inguinal adipose tissue were increased in response to ICE and that skeletal muscle FFA oxidative capacity elevated via increases in PPARG coactivator 1 alpha (PGC-1α) and p38 mitogen-activated protein kinases (p38MAPK) in response to ICE, thereby offsetting increases in FFA delivered to the circulation via increased lipolysis. Further evidence for ICE in promoting insulin sensitivity and glucose homeostasis has been provided by trials in rodent and human subjects. Although the literature is currently limited, human trials have demonstrated a pattern of decreased fasting glucose and increased insulin sensitivity assessed by glucose tolerance tests in response to ICE [16,30].

6. Effects of Exercise During ICE

Health-conscious individuals implementing intermittent cold exposure (ICE) are also likely to include other lifestyle practices to improve physical function. Three of our included ICE studies also examined the effects of exercise and ICE in combination, so we’ve reported those effects in Table 5 and discuss them here. Again, however, the reports from Arnold et al. and Deshaies et al. originate from the same cohort of animals [19,20]. Arnold and Deshaies report decreased bodyweight (BW) in response to ICE alone [19,20]. While Weng et al. does not report BW, they do note a trend for increased subcutaneous white adipose tissue (sWAT) [36], where altogether the variable effects of ICE on BW and fat mass are preserved in relation to the larger body of literature. Exercise was shown to reduce BW [19,20] but resulted no change in sWAT when sWAT trended higher with ICE alone [36]. In all studies, the combination of ICE and exercise did not provide any additional benefit with respect to reduced bodyweight or sWAT. More generally, the combination of exercise and ICE blunts the effect of ICE alone, where the combination provides the same benefit of exercise alone. Unfortunately, all three studies examined the effects of exercise during the CE associated with ICE and it’s likely that the heat generated during exercise simply blunted the thermogenic stress associated with ICE. There may be increased potential to derive a combinatory benefit of exercise and ICE if the exercise bout is implemented outside of the ICE bout.

7. Conclusion and Future Directions

The global obesity and type 2 diabetes epidemics constitute a grave threat to public health and demand effective interventions. Efforts to develop pharmaceuticals and decades of generic advice on lifestyle modification (i.e. “eat less, move more”) have thus far not succeeded in favorably altering the global trajectory toward ever-increasing rates of obesity and type 2 diabetes. Given the magnitude of the health-care and economic burdens associated with metabolic dysfunction, the need for effective prevention and treatment has never been more acute.
Intermittent cold exposure (ICE), due to its effects on energy expenditure (EE), seems a logical avenue of approach to combat obesity and its associated pathologies as cold exposure induces a metabolic energy demand. While ICE has received attention in the context of adipose tissue biology related to its effect on brown adipose tissue (BAT) thermogenesis, recent experimental evidence indicates that white adipose tissue (WAT) also engages a set of adaptive responses to cold challenge by becoming more thermogenic, a phenomenon referred to as “beiging” [41]. These adaptive responses at the level of gene expression provide a mechanistic basis for understanding how changes in WAT could underlie alterations in total energy expenditure and energy intake. With respect to our review of the ICE related literature, ICE clearly increases BAT activity and generally increases BAT mass. Further, ICE certainly transitions WAT to a beige phenotype based both on morphology and molecular signaling, but also seems to promote increased adiposity. As a mechanism for weight loss, the evidence does not support ICE; however, the trend for improved metabolic outcomes in response to ICE may still indicate its potential as an anti-diabetic intervention.
The above conclusions are based on a sparse literature in human subjects, where the majority of research examines rodent models housed in cold rooms. In our opinion, there is a need for an expansion of scientific investigation into the modalities of ICE most likely to be implemented by humans. The most likely option would be cold water immersion, which provides a much more intense bout of cold exposure with a reduced duration. This is not to say that all future research should utilize human subjects, but that the effects of ICE via cold water immersion would likely provide the most relevant outcomes in relation to what would most likely be implemented. Lastly, relevant investigations of cold-water immersion, in humans or rodents, would provide a methodological basis for future research aimed at parsing the effects of ICE intensity, duration, and frequency, similar to the literature on other lifestyle modifications such as exercise.

Author Contributions

Conceptualization, M.S. and S.F.; investigation, M.S. and S.F.; writing—original draft preparation, M.S. and S.F.; writing—review and editing, M.S. and S.F. All authors have read and agreed to the published version of the manuscript.

Funding

Dr. Scott is funded by the NCCIH (T32 AT 004094).

Conflicts of Interest

The authors declare no conflict of interest.”

References

  1. Grundy, S.M. Adipose Tissue and Metabolic Syndrome: Too Much, Too Little or Neither. Eur J Clin Invest 2015, 45, 1209. [Google Scholar] [CrossRef] [PubMed]
  2. Fahed, G.; Aoun, L.; Zerdan, M.B.; Allam, S.; Zerdan, M.B.; Bouferraa, Y.; Assi, H.I. Metabolic Syndrome: Updates on Pathophysiology and Management in 2021. Int J Mol Sci 2022, 23. [Google Scholar] [CrossRef] [PubMed]
  3. Qian, T.; Sheng, X.; Shen, P.; Fang, Y.; Deng, Y.; Zou, G. Mets-IR as a Predictor of Cardiovascular Events in the Middle-Aged and Elderly Population and Mediator Role of Blood Lipids. Front Endocrinol (Lausanne) 2023, 14, 1224967. [Google Scholar] [CrossRef] [PubMed]
  4. Kouvari, M.; D’cunha, N.M.; Travica, N.; Sergi, D.; Zec, M.; Marx, W.; Naumovski, N. Metabolic Syndrome, Cognitive Impairment and the Role of Diet: A Narrative Review. Nutrients 2022, 14. [Google Scholar] [CrossRef] [PubMed]
  5. Sakers, A.; De Siqueira, M.K.; Seale, P.; Villanueva, C.J. Adipose-Tissue Plasticity in Health and Disease. Cell 2022, 185, 419–446. [Google Scholar] [CrossRef] [PubMed]
  6. Hauner, H. The Mode of Action of Thiazolidinediones. Diabetes Metab Res Rev 2002, 18 (Suppl 2). [Google Scholar] [CrossRef] [PubMed]
  7. Ahmadian, M.; Suh, J.M.; Hah, N.; Liddle, C.; Atkins, A.R.; Downes, M.; Evans, R.M. PPARγ Signaling and Metabolism: The Good, the Bad and the Future. Nat Med 2013, 19, 557–566. [Google Scholar] [CrossRef] [PubMed]
  8. Jaacks, L.M.; Vandevijvere, S.; Pan, A.; McGowan, C.J.; Wallace, C.; Imamura, F.; Mozaffarian, D.; Swinburn, B.; Ezzati, M. The Obesity Transition: Stages of the Global Epidemic. Lancet Diabetes Endocrinol 2019, 7, 231. [Google Scholar] [CrossRef] [PubMed]
  9. Néma, J.; Zdara, J.; Lašák, P.; Bavlovič, J.; Bureš, M.; Pejchal, J.; Schvach, H. Impact of Cold Exposure on Life Satisfaction and Physical Composition of Soldiers. BMJ Mil Health 2023, e002237. [Google Scholar] [CrossRef]
  10. Van Der Lans, A.A.J.J.; Boon, M.R.; Haks, M.C.; Quinten, E.; Schaart, G.; Ottenhoff, T.H.; van Marken Lichtenbelt, W.D. Cold Acclimation Affects Immune Composition in Skeletal Muscle of Healthy Lean Subjects. Physiol Rep 2015, 3. [Google Scholar] [CrossRef]
  11. L, J.; D, P.; S, H.; B, U.; P, S.; V, Z.; J, K. Immune System of Cold-Exposed and Cold-Adapted Humans. Eur J Appl Physiol Occup Physiol 1996, 72, 445–450. [Google Scholar] [CrossRef] [PubMed]
  12. Van Tulleken, C.; Tipton, M.; Massey, H.; Harper, C.M. Open Water Swimming as a Treatment for Major Depressive Disorder. BMJ Case Rep 2018, 2018. [Google Scholar] [CrossRef] [PubMed]
  13. Castellani, J.W.; Young, A.J. Human Physiological Responses to Cold Exposure: Acute Responses and Acclimatization to Prolonged Exposure. Auton Neurosci 2016, 196, 63–74. [Google Scholar] [CrossRef] [PubMed]
  14. Fenzl, A.; Kiefer, F.W. Brown Adipose Tissue and Thermogenesis. Horm Mol Biol Clin Investig 2014, 19, 25–37. [Google Scholar] [CrossRef] [PubMed]
  15. Blondin, D.P.; Labbé, S.M.; Phoenix, S.; Guérin, B.; Turcotte, É.E.; Richard, D.; Carpentier, A.C.; Haman, F. Contributions of White and Brown Adipose Tissues and Skeletal Muscles to Acute Cold-Induced Metabolic Responses in Healthy Men. J Physiol 2015, 593, 701–714. [Google Scholar] [CrossRef] [PubMed]
  16. Søberg, S.; Löfgren, J.; Philipsen, F.E.; Jensen, M.; Hansen, A.E.; Ahrens, E.; Nystrup, K.B.; Nielsen, R.D.; Sølling, C.; Wedell-Neergaard, A.S.; et al. Altered Brown Fat Thermoregulation and Enhanced Cold-Induced Thermogenesis in Young, Healthy, Winter-Swimming Men. Cell Rep Med 2021, 2, 100408. [Google Scholar] [CrossRef] [PubMed]
  17. Cheng, L.; Wang, J.; Dai, H.; Duan, Y.; An, Y.; Shi, L.; Lv, Y.; Li, H.; Wang, C.; Ma, Q.; et al. Brown and Beige Adipose Tissue: A Novel Therapeutic Strategy for Obesity and Type 2 Diabetes Mellitus. Adipocyte 2021, 10, 48–65. [Google Scholar] [CrossRef]
  18. Liu, X.; Zhang, Z.; Song, Y.; Xie, H.; Dong, M. An Update on Brown Adipose Tissue and Obesity Intervention: Function, Regulation and Therapeutic Implications. Front Endocrinol (Lausanne) 2023, 13. [Google Scholar] [CrossRef]
  19. Arnold, J.; Richard, D. Exercise during Intermittent Cold Exposure Prevents Acclimation to Cold Rats. J Physiol 1987, 390, 45–54. [Google Scholar] [CrossRef]
  20. Deshaies, Y.; Arnold, J.; Richard, D. Lipoprotein Lipase in Adipose Tissues of Rats Running during Cold Exposure. J Appl Physiol (1985) 1988, 65, 549–554. [Google Scholar] [CrossRef]
  21. Doi, K.; Kuroshima, A. Lasting Effect of Infantile Cold Experience on Cold Tolerance in Adult Rats. Jpn J Physiol 1979, 29, 139–150. [Google Scholar] [CrossRef] [PubMed]
  22. Harri, M.; Dannenberg, T.; Oksanen-Rossi, R.; Hohtola, E.; Sundin, U. Related and Unrelated Changes in Response to Exercise and Cold in Rats: A Reevaluation. J Appl Physiol Respir Environ Exerc Physiol 1984, 57, 1489–1497. [Google Scholar] [CrossRef] [PubMed]
  23. Yahata, T.; Kuroshima, A. Metabolic Cold Acclimation after Repetitive Intermittent Cold Exposure in Rat. Jpn J Physiol 1989, 39, 215–228. [Google Scholar] [CrossRef] [PubMed]
  24. Yoo, H.S.; Qiao, L.; Bosco, C.; Leong, L.H.; Lytle, N.; Feng, G.S.; Chi, N.W.; Shao, J. Intermittent Cold Exposure Enhances Fat Accumulation in Mice. PLoS One 2014, 9. [Google Scholar] [CrossRef] [PubMed]
  25. Qiao, L.; Yoo, H.S.; Bosco, C.; Lee, B.; Feng, G.S.; Schaack, J.; Chi, N.W.; Shao, J. Adiponectin Reduces Thermogenesis by Inhibiting Brown Adipose Tissue Activation in Mice. Diabetologia 2014, 57, 1027–1036. [Google Scholar] [CrossRef] [PubMed]
  26. Ravussin, Y.; Xiao, C.; Gavrilova, O.; Reitman, M.L. Effect of Intermittent Cold Exposure on Brown Fat Activation, Obesity, and Energy Homeostasis in Mice. PLoS One 2014, 9. [Google Scholar] [CrossRef] [PubMed]
  27. Blondin, D.P.; Labbé, S.M.; Tingelstad, H.C.; Noll, C.; Kunach, M.; Phoenix, S.; Guérin, B.; Turcotte, É.E.; Carpentier, A.C.; Richard, D.; et al. Increased Brown Adipose Tissue Oxidative Capacity in Cold-Acclimated Humans. J Clin Endocrinol Metab 2014, 99, E438. [Google Scholar] [CrossRef] [PubMed]
  28. Wang, T.Y.; Liu, C.; Wang, A.; Sun, Q. Intermittent Cold Exposure Improves Glucose Homeostasis Associated with Brown and White Adipose Tissues in Mice. Life Sci 2015, 139, 153. [Google Scholar] [CrossRef] [PubMed]
  29. Bai, Z.; Wuren, T.; Liu, S.; Han, S.; Chen, L.; McClain, D.; Ge, R.L. Intermittent Cold Exposure Results in Visceral Adipose Tissue “Browning” in the Plateau Pika (Ochotona Curzoniae). Comp Biochem Physiol A Mol Integr Physiol 2015, 184, 171–178. [Google Scholar] [CrossRef] [PubMed]
  30. Gibas-Dorna, M.; Checinska, Z.; Korek, E.; Kupsz, J.; Sowinska, A.; Krauss, H. Cold Water Swimming Beneficially Modulates Insulin Sensitivity in Middle-Aged Individuals. J Aging Phys Act 2016, 24, 547–554. [Google Scholar] [CrossRef]
  31. Tsibul’nikov, S.Y.; Maslov, L.N.; Naryzhnaya, N. V.; Ivanov, V. V.; Lishmanov, Y.B. Specific Features of Adaptation of Rats to Chronic Cold Treatment. Doklady Biological Sciences 2016, 470, 214–216. [Google Scholar] [CrossRef] [PubMed]
  32. Blondin, D.P.; Daoud, A.; Taylor, T.; Tingelstad, H.C.; Bézaire, V.; Richard, D.; Carpentier, A.C.; Taylor, A.W.; Harper, M.-E.; Aguer, C.; et al. Four-Week Cold Acclimation in Adult Humans Shifts Uncoupling Thermogenesis from Skeletal Muscles to Brown Adipose Tissue. J Physiol 2017, 595, 2099–2113. [Google Scholar] [CrossRef] [PubMed]
  33. Presby, D.M.; Jackman, M.R.; Rudolph, M.C.; Sherk, V.D.; Foright, R.M.; Houck, J.A.; Johnson, G.C.; Orlicky, D.J.; Melanson, E.L.; Higgins, J.A.; et al. Compensation for Cold-Induced Thermogenesis during Weight Loss Maintenance and Regain. Am J Physiol Endocrinol Metab 2019, 316, E977–E986. [Google Scholar] [CrossRef]
  34. Zhang, L.; An, G.; Wu, S.; Wang, J.; Yang, D.; Zhang, Y.; Li, X. Long-Term Intermittent Cold Exposure Affects Peri-Ovarian Adipose Tissue and Ovarian Microenvironment in Rats. J Ovarian Res 2021, 14. [Google Scholar] [CrossRef] [PubMed]
  35. McKie, G.L.; Shamshoum, H.; Hunt, K.L.; Thorpe, H.H.A.; Dibe, H.A.; Khokhar, J.Y.; Doucette, C.A.; Wright, D.C. Intermittent Cold Exposure Improves Glucose Homeostasis despite Exacerbating Diet-Induced Obesity in Mice Housed at Thermoneutrality. J Physiol 2022, 600, 829–845. [Google Scholar] [CrossRef] [PubMed]
  36. Weng, X.; Wang, C.; Yuan, Y.; Wang, Z.; Kuang, J.; Yan, X.; Chen, H. Effect of Cold Exposure and Exercise on Insulin Sensitivity and Serum Free Fatty Acids in Obese Rats. Med Sci Sports Exerc 2023. [Google Scholar] [CrossRef] [PubMed]
  37. Brychta, R.J.; Chen, K.Y. Cold-Induced Thermogenesis in Humans. Eur J Clin Nutr 2017, 71, 345. [Google Scholar] [CrossRef] [PubMed]
  38. van Marken Lichtenbelt, W.D.; Schrauwen, P. Implications of Nonshivering Thermogenesis for Energy Balance Regulation in Humans. Am J Physiol Regul Integr Comp Physiol 2011, 301, 285–296. [Google Scholar] [CrossRef]
  39. Zeyl, A.; Stocks, J.M.; Taylor, N.A.S.; Jenkins, A.B. Interactions between Temperature and Human Leptin Physiology in Vivo and in Vitro. Eur J Appl Physiol 2004, 92, 571–578. [Google Scholar] [CrossRef]
  40. Gibas-Dorna, M.; Checinska, Z.; Korek, E.; Kupsz, J.; Sowinska, A.; Wojciechowska, M.; Krauss, H.; Piątek, J. Variations in Leptin and Insulin Levels within One Swimming Season in Non-Obese Female Cold Water Swimmers. Scand J Clin Lab Invest 2016, 76, 486–491. [Google Scholar] [CrossRef]
  41. Wu, J.; Boström, P.; Sparks, L.M.; Ye, L.; Choi, J.H.; Giang, A.-H.; Khandekar, M.; Virtanen, K.A.; Nuutila, P.; Schaart, G.; et al. Beige Adipocytes Are a Distinct Type of Thermogenic Fat Cell in Mouse and Human. Cell 2012, 150, 366–376. [Google Scholar] [CrossRef] [PubMed]
  42. Timmons, J.A.; Wennmalm, K.; Larsson, O.; Walden, T.B.; Lassmann, T.; Petrovic, N.; Hamilton, D.L.; Gimeno, R.E.; Wahlestedt, C.; Baar, K.; et al. Myogenic Gene Expression Signature Establishes That Brown and White Adipocytes Originate from Distinct Cell Lineages. Proceedings of the National Academy of Sciences 2007, 104, 4401–4406. [Google Scholar] [CrossRef] [PubMed]
  43. Rosen, E.D.; Spiegelman, B.M. What We Talk About When We Talk About Fat. Cell 2014, 156, 20–44. [Google Scholar] [CrossRef] [PubMed]
  44. Straub, L.G.; Scherer, P.E. Metabolic Messengers: Adiponectin. Nat Metab 2019, 1, 334–339. [Google Scholar] [CrossRef] [PubMed]
  45. Ye, L.; Wu, J.; Cohen, P.; Kazak, L.; Khandekar, M.J.; Jedrychowski, M.P.; Zeng, X.; Gygi, S.P.; Spiegelman, B.M. Fat Cells Directly Sense Temperature to Activate Thermogenesis. Proceedings of the National Academy of Sciences 2013, 110, 12480–12485. [Google Scholar] [CrossRef]
  46. Boström, P.; Wu, J.; Jedrychowski, M.P.; Korde, A.; Ye, L.; Lo, J.C.; Rasbach, K.A.; Boström, E.A.; Choi, J.H.; Long, J.Z.; et al. A PGC1-α-Dependent Myokine That Drives Brown-Fat-like Development of White Fat and Thermogenesis. Nature 2012, 481, 463–468. [Google Scholar] [CrossRef] [PubMed]
  47. Rosenwald, M.; Perdikari, A.; Rülicke, T.; Wolfrum, C. Bi-Directional Interconversion of Brite and White Adipocytes. Nat Cell Biol 2013, 15, 659–667. [Google Scholar] [CrossRef]
  48. Roberts, C.K.; Hevener, A.L.; Barnard, R.J. Metabolic Syndrome and Insulin Resistance: Underlying Causes and Modification by Exercise Training. In Comprehensive Physiology; Wiley, 2013; pp. 1–58. [Google Scholar]
  49. Bornfeldt, K.E.; Tabas, I. Insulin Resistance, Hyperglycemia, and Atherosclerosis. Cell Metab 2011, 14, 575–585. [Google Scholar] [CrossRef]
Table 1. ICE Methods.
Table 1. ICE Methods.
Year First
Author		 Citation Methods
Model Groups ICE
Modality		 ICE Freq. ICE Intensity ICE Duration ICE
Intervention
1979 Doi [21] Rats 1. Control Newborn
2. Control Adult
3. ICE Newborn
4. ICE Adult		 Air daily 5°C 4hrs 14 days
1984 Harri [22] Rats 1. Control
2. CCE
3. ICE
4. Control Exercise
5. CCE Exercise		 Air 5d/wk -20°C 1hr ≥6wks
1986 Arnold [19] Rats 1. Control
2. ICE
3. Control Exercise
4. ICE Exercise		 Air daily -5°C 2hrs 28 days
1988 Deshaies [20] Rats 1. Control
2. ICE
3. Control Exercise
4. ICE Exercise		 Air daily -5°C 2hrs 28 days
1989 Yahata [23] Rats 1. Control
2. CCE
3. ICE		 Air daily 5°C 6hs or 2hrs 28 days
2014 Yoo [24] Mice 1. Control
2. ACE
3. ICE		 Air daily 4°C 6hrs 12-14 days
2014 Qiao [25] Mice 1. Control ICE
2. Adipoq-/- ICE		 Air daily 4°C 6hrs 10 days
2014 Ravussin [26] Mice 1. Control
2. ICE 1hr
3. ICE 4hr
4. ICE 8hr		 Air 3d/wk 4°C 1, 4, or 8hrs 10-11wks
2014 Blondin [27] Humans Pre vs Post Liquid cooled suit 5d/wk 18°C 2hrs 4wks
2015 Wang [28] Mice 1. Control
2. ICE		 Air 5d/wk 4°C 2hrs 14wks
2015 Bai [29] Platue Pika (Rodent)
Simulated low air pressure (4100M)		 1. Control
2. ICE		 Air daily 5-6°C 4hrs 15 days
2016 Gibas-Dorna [30] Humans 1. Controls
2. Winter Swimmers
(Pre vs Post)		 Winter Swimming ≥2d/wk 1-10°C 5-15min ~6 months
2016 Tsibui'nikov [31] Rats 1. Control
2. CCE
3. ICE (1.5hrs)
4. ICE (8hrs)		 Air daily 4°C 1.5 or 8hrs 4wks
2017 Blondin [32] Humans Pre vs Post ICE Liquid cooled suit daily 10°C 2hrs 4wks
2019 Presby [33] Obese Mice
Caloric Restriction During ICE
24hr ad libitum after ICE		 1. Caloric Restriction
2. ICE + Caloric Restriction		 Air 5d/wk 4°C 1.5hrs 3wks
2021 Soberg [16] Humans 1. Controls
2. Winter Swimmers		 Winter Swimming 2-3d/wk NA 11min At least 1 season
2021 Zhang [34] Rats 1. Control
2. ICE		 Air daily 10°C 4hrs 2wks
2022 McKie [35] Obese Mice 1. HFD
2. ICE + HFD		 Air 5d/wk 4°C 1hr 4wks
2023 Weng [36] Obese Rats 1.Control
2.Exercise
3.ACE
4.ACE+Exercise
5.CCE
6.CCE+Exercise
7.ICE
8.ICE+Exercise		 Air daily 3-4°C 4hrs 5wks
2023 Nema [9] Humans (Soldiers) 1. Control Pre vs Post
2. ICE Pre vs Post
CWI and CS		 CWI ≥1/wk
CS ≥4/wk		 CWI≤6°C
CS≤10°C		 CWI ≥3min
CS ≥30s		 8wks
Legend: cold exposure (CE), chronic cold exposure (CCE), intermittent cold exposure (ICE), acute cold exposure (ACE), Cold Water Immersion (CWI), Cold Shower (CS), Not Applicable (NA), High Fat Diet (HFD).
Table 3. ICE effects on adipose genes and proteins.
Table 3. ICE effects on adipose genes and proteins.
Year First
Author		 Citation ICE Outcomes Model
1984 Harri [22] ↑Citrate synthase in BAT, ↑Cytochrome C oxidase in BAT Rats
1988 Deshaies [20] ↔eWAT LPL Rats
1989 Yahata [23] ↑BAT glucagon Rats
2014 Yoo [24] ↑iWAT Ucp1 and UCP1, ↑iWAT lipogenic gene expression Mice
2014 Qiao [25] Adipoq-/- : ↑iWAT UCP1, ↑BAT UCP1 and Lipogenic protein at RT, ↓BAT UCP1 and PGC1α protein during CE Mice
2014 Ravussin [26] Cohort 1 (1 or 4hrs ICE): ↑BAT Ucp1 (4hrs), ↑BAT Pgc1α (4hrs), ↓serum leptin (4hrs)
Cohort 2 (4 or 8hrs ICE): ↑BAT UCP1 (4&8hrs)		 Mice
2015 Wang [28] ↑sWAT UCP1 and PGC1α, ↔BAT UCP1 (trended↑) Mice
2015 Bai [29] ↑BAT thermogenic gene expression (Pgc1α, Dio2, Cidea) and adipogenic gene expression (Adipoq, Cebpα, Ppary, Fabp4), ↑ pericardial WAT thermogenic gene expression (Ucp1 and Pgc1α), ↑pericardial WAT UCP1 staining and mito activity genes (Cox8 and ATP5α), ↔ serum leptin Platue Pika (Rodent)
2019 Presby [33] ↑sWAT Dio2, ↑sWAT UCP1, ↑BAT Dio2, ↑BAT UCP1 Obese Mice
Caloric Restriction During ICE
24hr ad libitum after ICE
2021 Zhang [34] ↑peri-ovarian adipose thermogenic gene expression Rats
2023 Weng [36] ↔ iWAT ATGL activity, ↑iWAT LPL activity Obese Rats
Legend: increased (↑), no change (↔), decreased (↓), cold exposure (CE), intermittent cold expsure (ICE), white adipose tissue (WAT), epididymal WAT (eWAT), inguinal WAT (iWAT), subcutaneous WAT (sWAT), brown adipose tissue (BAT), messenger ribonucleic acid (mRNA), lipoprotein lipase (LPL, protein), uncoupling protein 1 (UCP1, protein; Ucp-1, mRNA), PPARG coativator 1 alpha (PGC1α, protein; Pgc1α, mRNA), iodothyronine deiodinase 2 (Dio2, mRNA), cell death inducing DFFA like effector a (Cidea, mRNA), adiponectin (Adipoq, mRNA), CCAAT enhancer binding protein alpha (Cebpα, mRNA), fatty acid binding protein 4 (Fabp4, mRNA), cytochrome c oxidase subunit 8A (Cox8, mRNA), ATP synthase F1 subunit alpha (Atp5α, mRNA), adipose triglyceride lipase (ATGL, protein).
Table 4. ICE effects on systemic factors.
Table 4. ICE effects on systemic factors.
Year First
Author		 Citation ICE Outcomes Model
1988 Deshaies [20] ↓serum cholesterol, ↔HDL Rats
1989 Yahata [23] ↑plasma glucagon, ↑corticosterone Rats
2014 Yoo [24] ↔IS, ↑Liver TGs and Hepatic TG release, ↑DNL Mice
2014 Ravussin [26] Cohort 1 (1 or 4hrs ICE): ↑BAT glucose uptake
Cohort 2 (4 or 8hrs ICE): ↑FFA (4&8hrs), ↓insulin		 Mice
2014 Blondin [27] ↓BG, ↓Cortisol Humans
2015 Wang [28] ↑glucose tolerance, ↑insulin sensitivity Mice
2015 Bai [29] ↔serum glucose, ↔serum TG Platue Pika (Rodent)
2016 Gibas-Dorna [30] ↑IS during the portion of the winter swimming season where water tempterature was <8°C Humans
2017 Blondin [32] ↔FG Humans
2021 Soberg [16] Winter Swimmers had: ↓plasma glucose during IGTT Humans
2022 McKie [35] ↑glucose tolerance, ↑insulin and c-peptide in response to glucose, ↔IS Obese Mice
2023 Weng [36] ↔BG, ↔ insulin, ↓HOMA-IR, ↔FFA Obese Rats
Legend: increased (↑), no change (↔), decreased (↓), intermittent cold exposure (ICE), brown adipose tissue (BAT), high density lipoproteins (HDL), insulin sensitivity (IS), triglyceride (TG), denovolipogenesis (DNL), blood glucose (BG), fasting glucose (FG), intravenous glucose tolerance test (IGTT), homoestatic model assesment for insulin resistance (HOMA-IR), free fatty acid (FFA).
Table 5. Combined effects of ICE and exercise.
Table 5. Combined effects of ICE and exercise.
Year First
Author		 Citation Intervention Outcomes Model
1986 Arnold [19] ICE ↓BW, ↓FFM, ↓FM, ↑BAT, ↑EE, ↑EI Rats
Ex ↓↓BW, ↓FFM, ↓BAT, ↓↓FM, ↔ EE, ↓EI
ICE+Ex ↓↓BW, ↓FFM, ↓↓FM, ↔BAT, ↓EI, ↔EE
1988 Deshaies [20] ICE ↓BW, ↓eWAT, ↑BAT, ↑EI, ↔TG, ↓Cholesterol Rats
Ex ↓↓BW, ↓↓eWAT, ↔ BAT, ↓EI, ↓TG, ↔Cholesterol
ICE+Ex ↓↓BW, ↓↓eWAT, ↔ BAT, ↓EI, ↓TG, ↔ Cholesterol
2023 Weng [36] ICE ↔sWAT (trend↑), ↑sWAT LPL activity, ↑ muscle PGC1α, ↑ muscle p38MAPK Obese Rats
Ex ↔sWAT, ↑sWAT LPL activity, ↑ muscle PGC1α, ↑ muscle p38MAPK
ICE+Ex ↔sWAT, ↑↑sWAT LPL activity
Legend: increased (↑), significantly increased compared to ↑ ( ↑↑), no change (↔), decreased (↓), significantly decreased compared to ↓ (↓↓), treadmill running exercise (Ex), Ex during ICE (ICE+Ex), intermittent cold exposure (ICE), body weight (BW), fat mass (FM), fat free mass (FFM), white adipose tissue (WAT), epididymal WAT (eWAT), subcutaneous WAT (WAT), brown adipose tissue (BAT), energy expenditure (EE), energy intake (EI), triglyceride (TG), lipoprotein lipase (LPL), PPARG coativator 1 alpha (PGC1α, protein), mammalian p38 mitogen-activated protein kinase (p38MAPK, protein).
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
Prerpints.org logo

Preprints.org is a free preprint server supported by MDPI in Basel, Switzerland.

facebook logotwitter logolinkedin logo
MDPI Initiatives
Important Links
Subscribe

Disclaimer

Terms of Use

Privacy Policy

© 2024 MDPI (Basel, Switzerland) unless otherwise stated