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Aquaporin Expression and Regulation in Clinical and Experimental Sepsis

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Submitted:

24 November 2023

Posted:

27 November 2023

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Abstract
Sepsis is an inflammatory disorder caused by the host’s dysfunctional response to infection. Septic patients present diverse clinical characteristics, and in the recent years it has been the main cause of death in intensive care units (ICU). Aquaporins, membrane proteins with role in water transportation, have been reported to participate in numerous biological processes. Their role in sepsis progression has been studied extensively. This review aims to examine recent literature on aquaporin expression and regulation in clinical sepsis, as well as established experimental models of sepsis. We will present how sepsis affects aquaporin expression at the molecular and protein level. Moreover, we will delve into the importance of aquaporin regulation at the transcriptional, post-transcriptional, translational, and post-translational level in sepsis, by presenting data on aquaporin regulation by non-coding RNAs and selected chemical molecules. Finally, we will focus on the importance of aquaporin single nucleotide polymorphisms in the setting of sepsis.
Keywords: 
aquaporin
;  
sepsis
;  
ARDS
;  
SNP
;  
lnRNA
;  
miRNA
Subject: 
Medicine and Pharmacology  -   Pathology and Pathobiology

1. Introduction

Aquaporins (AQPs) compose a family of membrane proteins whose main function is the regulation of water transportation. To this day, 13 unique mammalian aquaporins have been identified. Several members of the aquaporin protein family are known as aquaglyceroporins and retain a role in the passive transport of glycerol and other small solutes, such as urea and carbon dioxide. AQPs are homo-tetramers, that is they consist of four identical monomers of approximately 30 kDa, which are the molecule’s structural backbone, each having the ability to function as a channel [1]. AQPs are implicated in a plethora of biological functions, which differ among water selective AQPs and the aquaglyceroporins [2]. Transepithelial fluid, cell migration, and brain edema have been connected to water selective AQPs. On the other hand, aquaglyceroporins are integral in cell proliferation, adipocyte metabolism, and epidermal water retention. In mammals, aquaporins have been detected primarily in the lung, kidney, eye, and brain and have been examined as possible therapeutic targets in disorders characterized by dysregulation of water homeostasis [3,4]. Most notably, AQP1 is expressed in the brain, kidney, lungs and eyes, AQP2 predominantly in the kidneys, AQP3 in the kidneys, the digestive tract, and immune cells, AQP4 in the central nervous system, kidneys and digestive tract, AQP5 in the lungs and salivary glands, AQP6 in renal epithelia, AQP7 in the liver, kidneys, and cardiac muscle, AQP8 in the liver, kidneys, and pancreas, AQP9 in immune cells, AQP10 in the gastrointerstinal tract, AQP11 in the endoplasmic reticulum membrane, and AQP12 in the pancreas, intestine, and stomach [5].
In recent years, involvement of AQPs in inflammatory processes has been firmly established [6]. Sepsis is an inflammatory disorder and is defined as a life-threatening organ dysfunction caused by the host’s dysregulated response to infection [7]. The disorder’s immense complexity results in high mortality rates. More importantly, in recent years sepsis has been identified as the primary cause of mortality in intensive care units (ICU) [8]. Furthermore, one of the main difficulties presented by sepsis is the fact that patients’ clinical characteristics do not correlate fully with their outcome. Specifically, patients die at every stage regardless of their clinical characteristics [9].
The aim of this review is to provide a clear understanding on the recent advances regarding the expression and regulation of aquaporins in both clinical and experimental models of sepsis. We present an overview regarding AQP1, AQP2, AQP3, AQP4, AQP5, and AQP9 expression and regulation at the transcriptional, translational, and post-translation levels. Reviewed literature covers septic patients and experimental models of sepsis, including mice or rats exposed to lipopolysaccharide (LPS), mice or rats on which cecal-ligation and puncture (CLP) was performed, and human cells exposed to LPS.

2. Aquaporin Expression in Clinical and Experimental Models of Sepsis

Not many studies have investigated the expression of aquaporins in a clinical septic setting. The results of these studies have, however, demonstrated that the expression of aquaporins is dysregulated in sepsis, as well as other pathological conditions that arise due to sepsis development. In a clinical setting, our research group has demonstrated that leukocyte AQP1 mRNA expression levels were significantly elevated in ICU patients who developed sepsis, compared to non-septic ICU patients [10]. Matshushima and colleagues demonstrated that AQP9 expression was significantly upregulated in PMNs of patients diagnosed with systemic inflammatory response syndrome (SIRS) [11]. In whole blood samples of sepsis non-survivors, AQP5 mRNA expression was shown to be increased in comparison to sepsis survivors [12]. In a cohort of twelve healthy human subjects, in whom eight were administered intravenous LPS, AQP9 mRNA levels in peripheral blood leukocytes were significantly elevated following LPS administration [13]. Recently, Xie and colleagues analyzed the expression of AQP9, among other genes, in the GSE32707 dataset. The dataset included mRNA expression data from whole blood samples of healthy individuals and patients with acute respiratory distress syndrome (ARDS). ARDS is a life-threatening disorder, common in the setting of sepsis, comprised of many pathological attributes, including diffuse endothelial injury, increased capillary permeability, and hypoxemia. Furthermore, ARDS constitutes one of the leading causes of death in the ICU [14,15]. Initial results demonstrated an upregulation of AQP9 mRNA expression in ARDS patients, although when validation analysis was performed on a separate group of ARDS patients and healthy individuals, no significant changes for AQP9 expression were detected [16]. Finally, AQP4 protein levels were found elevated in blood samples of patients suffering from sepsis-associated encephalopathy (SAE) [17]. Table 1 presents an overview of human studies on the expression of AQPs in the setting of sepsis and related disorders.
As opposed to human studies, more data exist from studies performed on various experimental septic models. In polymorphonuclear neutrophils (PMNs) isolated from healthy donors, stimulation with LPS resulted in an increase of AQP1 mRNA and protein expression [10]. Furthermore, da Silva and colleagues demonstrated that in primary human monocytes, exposure to LPS resulted in elevated levels of AQP9 mRNA. In addition, AQP9 and AQP3 mRNA upregulation was reported in LPS-exposed monocytes originally treated with phorbol myristate acetate (PMA) [20]. One of the most common complications of sepsis is lung injury. Aquaporin expression has been studied in several animal and cell models of sepsis-induced lung injury. Li et al. studied the expression of AQP1, AQP3, AQP4 and AQP5 in lungs of mice exposed to LPS. They reported that protein expression of AQP1 and AQP5 decreased significantly at 4 and 8 hours post LPS-exposure, while AQP3 and AQP4 protein levels remained unaltered [21]. Furthermore, AQP1 and AQP5 protein expression decreased in the alveoli of lung injury mice exposed to LPS for 12 hours [22]. Rump and colleagues demonstrated that in the same lung injury model, lung AQP1 expression was downregulated while mRNA expression of AQP5 remained unaltered [23]. Moreover, work from our research group has reported the expression patterns of aquaporins in several animal models of lung injury. Specifically, in LPS-exposed mice, mRNA expression and protein levels of AQP5 were found downregulated, while AQP1 expression remained unaffected. Interestingly, AQP9 expression was found to increase significantly, although no changes in the protein levels were identified [24]. Additionally, in rats, both mRNA and protein levels of AQP1 and AQP5 were found to decrease significantly in lung tissues following exposure to LPS [25]. Same results regarding AQP1 and AQP5 mRNA and protein expression were demonstrated in a cecal ligation and puncture (CLP) rat model by Guo and colleagues [26]. Furthermore, CLP-induced septic rats exhibited increased expressions of both AQP3 and AQP4 mRNA in the pulmonary vascular, while only protein expression of AQP3 was found elevated [27].
Expression of aquaporins has also been investigated in experimental models of sepsis-induced acute kidney injury. Wang et al. examined the expression of several aquaporins in renal tubular epithelial cells following LPS exposure for 12 hours. Among all studied aquaporins, only AQP1 and AQP2 mRNA expression was altered, showing a significant decrease [28]. Additionally, Li et al. reported a difference between AQP1 mRNA and protein levels in kidney tissues of LPS-induced rats. Specifically, AQP1 expression in kidney tissues was decreased following injury presenting a steady increase with time, while AQP1 protein expression in serum and kidney tissues was initially upregulated and diminished with time [29]. Kidney AQP2 protein levels have been shown to decrease in both CLP- and LPS-induced septic rats [30,31]. In rats injected with LPS, Olesen and colleagues showed that following treatment, AQP2 protein levels were downregulated in the cortex and the inner stripe of the outer medulla, while no significant changes were observed in the inner medulla [32]. Moreover, Wang and colleagues have demonstrated that in endotoxemia induced mice, protein expression of AQP2 and AQP3 decreased significantly in the kidneys following treatment with LPS [33]. Table 2 lists the experimental sepsis models that have studied the expression of AQPs.

3. Aquaporin Long Non-Coding RNAs and Micro RNAs in Clinical and Experimental Models of Sepsis

Many long non-coding RNAs (lncRNAs) and micro RNAs (miRNAs) have been described as regulators of aquaporin expression in different conditions including lung injury, acute kidney injury, cerebral ischemic reperfusion injury, and various cancer types. Recent endeavors have shed light on the involvement of aquaporin lncRNAs and miRNAs in the pathophysiology of sepsis. Long non-coding RNAs are non-protein coding transcripts consisting of over 200 nucleotides that can regulate gene expression on epigenetic, transcriptional, and post-transcriptional levels. lncRNAs are of great importance in a plethora of biological processes including chromosome modification, transcriptional regulation, as well as genomic imprinting [50]. On the other hand, microRNAs are small non-coding RNAs that are composed by an average of 22 nucleotides. Interaction of miRNAs with gene regions, mainly the 3’ untranslated region (3’ UTR), results in translational repression, although in certain occasions, gene activation has been reported [51,52]. As with lncRNAs, the involvement of miRNAs in many biological processes is well established. Most importantly, the pathophysiology of numerous human diseases has been connected to dysregulated expression of miRNAs [53].
In recent years, involvement of both lncRNAs and miRNAs in the regulation of sepsis pathophysiology has been examined more rigorously, with members of both RNA groups being promising diagnostic markers and potential therapeutic targets [54]. Initially, Fang et al. demonstrated that in the setting of sepsis, the lncRNA H19 functions as an AQP1 competitive endogenous RNA (ceRNA) in regulating miR-874, which directly interacts with AQP1. Expression levels of H19 and AQP1 were found to be diminished in the sera of septic patients, while miR-874 levels were elevated, showing a negative relationship with both H19 and AQP1 [18]. miR-874 is a novel anti-cancer miRNA. Through the inhibition of gene expression, miR-874 retains a key role in numerous cellular mechanisms, including but not limited to apoptosis, cell proliferation, and migration [55]. Interestingly, in sepsis-induced ARDS, lncRNA H19 was shown to have great value as an early diagnostic tool. Specifically, serum H19 levels of septic patients were decreased compared to healthy individuals, and, thus could be potentially used as a tool for early sepsis diagnosis [56]. The long non-coding RNA cancer susceptibility candidate 2 (CASC2) is another lnRNA that has been demonstrated to regulate AQP1 expression in lung carcinoma epithelial cells and lung injury mice models via the miR-144-3p/AQP1 axis [34]. In humans, CASC2 levels have been found decreased in septic patients compared to healthy individuals and could be utilized as a biomarker for disease severity and mortality [57].
LncRNA-5657 is also of great importance in the development of ARDS. Expression of lncRNA-5657 was found elevated in the bronchoalveolar lavage fluid (BALF) cells of patients with sepsis-induced ARDS [58]. Although lnc-5657 has not been shown to regulate aquaporin expression in sepsis-induced ARDS, a link between lnc-5657 and AQP4 has been identified in sepsis-associated encephalopathy (SAE). The main characteristic of SAE is diffuse cerebral dysfunction induced by the systemic response to infection [59]. Finally, AQP1 has been shown to be regulated by miR-126-5p. Healthy individuals exhibited elevated plasma levels of miR-126-5p compared to septic patients and sepsis-induced ARDS patients. Interestingly, patients suffering from sepsis-induced ARDS demonstrated decreased miR-126-5p levels compared to septic patients as well [60].
In sepsis-induced acute kidney injury, AQP2 expression has been shown to be regulated by miR-34b-5p. Serum levels of miR-34b-5p were found elevated in sepsis-induced acute kidney injury (AKI) patients. Additionally, the decreased AQP2 gene and protein expression found in human renal tubular epithelial cells (HK-2) treated with LPS were negatively regulated by miR-34b-5p, thus promoting apoptosis and inflammatory response [45].
In experimental sepsis models, upregulation of the lncRNA H19 has been demonstrated in CLP rats with sepsis-induced lung injury, resulting in the reduction of both apoptosis and pulmonary inflammation, prompting the authors to suggest its role as a possible therapeutic target [56]. Initial exposure of mice to LPS, induced apoptosis and resulted in decreased expression of both lncRNA CASC2 and AQP1, while miR-144-3p expression increased. CASC2 overexpression reversed the effects of LPS on AQP1 gene expression and reduced apoptosis, by regulating miR-144-3p, which binds to the 3’ UTR of the AQP1 gene [34]. The significance of lncRNA CASC2 in regulating expression levels during sepsis-induced lung injury has also been pinpointed in other studies. CASC2 has been shown to serve as a competing endogenous RNA of miR-27b and miR-152-3p, which downregulate transforming growth factor-β activated kinase 1 binding protein 2 (TAB2) and pyruvate dehydrogenase kinase 4 (PDK4), respectively in lung injury models [61,62]. In a murine LPS-induced acute lung injury model, overexpression of miR-126-5p in alveolar type II (ATII) cells was shown to ameliorate the reduction of AQP1 protein expression, which resulted from the LPS treatment [35]. In the lung tissue of a CLP-induced sepsis rat model, downregulation of lncRNA-5657 resulted in the reduction of lung inflammation caused by CLP [58]. Moreover, the elevated AQP4 protein levels found in cortical and hippocampal tissues of CLP-induced SAE mice could be reduced via the downregulation of lncRNA-5657 [17]. Finally, neuronal degradation and necrosis found in the hippocampus of CLP sepsis-induced rats were attenuated following lncRNA-5657 silencing [63]. As in the case of ARDS, lncRNA-5657 downregulation repressed the progression of SAE.
In an LPS-induced lung injury mouse model, the intravenous injection of a miR-34b-5p antagomir in vivo significantly inhibited miR-34b-5p up-regulation, reduced inflammatory cytokine release, decreased alveolar epithelial cell apoptosis, attenuated lung inflammation, and improved survival. The authors suggested that the AQP2 expression regulator miR-34b-5p may be a potential target for lung injury treatments [64].
AQP5 regulation by miRNAs has been examined in the lungs of an LPS-induced disseminated intravascular coagulation (DIC) rat model. DIC is defined as a systemic intravascular activation of coagulation that leads to fibrin formation and deposition in the microcirculation. DIC constitutes a frequents result of sepsis [65]. Zhang et al. reported that following LPS exposure, lung levels of AQP5 mRNA and protein expression decreased, while miR-96 and miR-330 expression levels were found elevated, suggesting that AQP5 is directly regulated by both miRNAs [48]. MicroRNA-96 is a member of the miR-183-96-182 cluster presenting a significant role in cell migration and tumor progression, while miR330 has been shown capable of acting as a suppressor or activator of numerous processes associated with the progression of malignancies [66,67].

4. Aquaporin Regulators in Experimental Models of Sepsis

A growing number of studies have explored the effect of various molecules on the regulation and function of aquaporins, and how they may relate to the attenuation of conditions that arise due to sepsis manifestation.
Many of these studies have focused on identifying molecules with prophylactic effects against sepsis-induced lung injury that act by regulating the expression of aquaporins. Several molecules with a regulatory effect on aquaporin 5 expression have been identified. Lipoxin A4 (LXA4) has been shown to alleviate sepsis-induced lung injury in both LPS-exposed mice and cecal ligation rat models [47,68]. Lipoxins are bioactive autacoid metabolites and are the byproducts of the reaction between arachidonic acid and lipoxygenase enzymes. Lipoxins form during inflammation and activate cellular pathways to elicit their anti-inflammatory role. Lipoxin A4 is naturally occurring and one of the two originally identified lipoxins [69,70]. More specifically, in the LPS-induced lung injury model, exposure to LPS originally resulted in decreased levels of AQP5 protein expression. Following treatment with lipoxin A4, AQP5 protein levels in the lung tissue of sepsis-induced mice increased significantly, which was possibly attributed to the reduction of p38 and c-Jun N-terminal kinase (JNK) phosphorylation. Hence, the authors concluded that LXA4 plays a protective role in LPS-induced lung injury by upregulating AQP5 expression, suggesting a potential new mechanism of LXA4 as an anti-inflammation therapy for the impairment of alveolar fluid transport in ALI [47].
AQP5 mRNA and protein expression can also be regulated by tanshinol. Tanshinol has been identified as the main active compound of Salvia miltiorrhiza Bunge, which is acknowledged by Chinese medicine as a treatment for cardiovascular diseases [71]. Sepsis-induced lung injury in rats following CLP resulted in diminished AQP5 mRNA and protein expression. Tanshinol treatment was able to reverse this effect and return AQP5 expression close to pre-treatment levels. Moreover, tanshinol attenuated the influx of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) concentrations in rat lung tissue, exhibiting an anti-inflammatory role. It was concluded that tanshinol may upregulate the expression of AQP5 by inhibiting the inflammatory cytokines and the phosphorylation of p38, therefore protecting the lung tissue of rats with sepsis [49].
Fasudil, a selective Rho kinase (ROCK) inhibitor, has been demonstrated to impact AQP5 expression in a similar fashion to tanshinol. Fasudil pre-treatment and subsequent exposure of mice to LPS resulting in lung injury demonstrated an upregulation of both AQP5 mRNA and protein levels, which were diminished in LPS-exposed mice. LPS-exposed mice pre-treated with fasudil demonstrated a downregulation of IL-6 in the lung, the peripheral blood, and in the bronchoalveolar fluid (BALF). Hence, fasudil seemed to alleviate LPS-induced lung injury by restoring AQP5 expression, to eliminate LPS-induced lung edema and prevent LPS-induced pulmonary inflammation by inhibiting inflammation in the lungs [46].
More recent studies have generated data in regard to other molecules that can attenuate sepsis-induced lung injury by regulating AQP1 expression. A study by Liang and colleagues demonstrated the role of the myocyte-specific enhancer factor 2C (MEF2C), a transcription factor and member of the Mef2 family, in the suppression of sepsis-induced lung injury in rats. They demonstrated that CLP-induced lung injury resulted in the reduction of MEF2C mRNA and protein levels, whereas MEF2C treatment attenuated the progress of lung injury, while simultaneously upregulating AQP1 mRNA and protein expression. Furthermore, serum concentrations of TNF-α, IL-1β and IL-6 were decreased, while concentrations were of IL-10 were elevated following MEF2C treatment. More importantly, inhibition of AQP1 expression reversed the effect of MEF2C on lung injury suppression, suggesting a possible connection between MEF2C and AQP1 expression in the progress on lung injury [40].
The hypoxia-inducible factor 1α (HIF-1α) holds a significant role in the regulation of AQP1 expression. In human pulmonary microvascular endothelial cells (HPMECs) exposed to LPS, AQP1 mRNA expression was elevated, as was its function, as was demonstrated by the increase of the cells’ volume. Interestingly, silencing of HIF1A expression in the LPS-induced cells attenuated AQP1 upregulation, thus indicating HIF1A as a potential therapeutic target [41].
In addition to the above-mentioned studies, several molecules have been demonstrated to impact simultaneously both AQP1 and AQP5 expression levels in the presence of lung injury. The estrogen steroid hormone estradiol can inhibit processes integral to lung injury development. Pre-treatment with estradiol of mice exposed to LPS resulted in a significant upregulation of both AQP1 and AQP5 mRNA and protein levels compared to the untreated LPS-induced lung injury group. Increases in AQP1 and AQP5 expression were accompanied by reduced oxidative stress and inflammatory responses [36]. Research from the same group attributed to soy isoflavone a similar role to that of estradiol in the regulation of AQP1 and AQP5 expression. Initially, LPS exposure of rats resulted in decreased AQP1 and AQP5 mRNA and protein levels in lung tissues. Pre-treatment of rats with increasing concentrations of soy isoflavone resulted in a dose-dependent upregulation of AQP1 and AQP5 mRNA and protein expression. Additionally, soy isoflavone was found to decrease TNF-α, IL-1β, and IL-6 levels, alleviating pulmonary edema and lung damage [37].
Another molecule connected to the upregulation of aquaporins is emodin, which is a naturally occurring anthraquinone derivative. Emodin can be isolated from Rheum palmatum or Polygonam multiflorum. It acts as a suppressor of various signaling pathways and has been shown to have anti-inflammatory properties in combination with other substances [72]. Cecal ligation puncture in rats decreased mRNA and protein expression levels of both AQP1 and AQP5. Pre-treatment of rats with emodin increased significantly both mRNA and protein expression levels at 12-hours post-CLP. At the same timepoint, emodin was shown to suppress sepsis-induced pulmonary apoptosis and had a positive effect on mortality [26,73].
Finally, hydrogen-rich saline has been shown to reverse the downregulation of AQP1 and AQP5 mRNA and protein expression in an LPS-induced lung injury rat model. Investigators suggested that hydrogen-rich saline participates in the inhibition of p38 MAPK and JNK, thus resulting in the upregulation of aquaporin expression [38]. Another study demonstrated that hydrogen-rich saline could suppress lung injury in LPS-exposed septic rats via apoptosis reduction [74].
Two recent studies focused on molecules regulating AQP3 and AQP4 expression in sepsis-induced lung injury. In both CLP-induced septic rats and LPS-treated pulmonary vein endothelial cells, AQP3 expression was significantly upregulated and correlated with increased pulmonary vascular permeability. Treatment with Ss-31, a novel antioxidant, resulted in decreased expression of AQP3 and pulmonary vascular permeability [27]. Furthermore, AQP4 expression has been reported to increase following sepsis-induced lung injury in LPS-exposed mice and CLP-rats [27,75]. In mice with LPS-induced lung injury, TGN-020 treatment, a selective AQP4 inhibitor, resulted in downregulation of AQP4, suppression of inflammatory cytokine production, and better survival rates [75].
As seen above, many studies have focused on sepsis-induced lung injury. However, studies exploring the possibility of various molecules in the regulation of aquaporins in sepsis-induced kidney injury also exist. One of the studied molecules is selenium, a trace element that mainly serves as an antioxidant and participates in combating inflammation [76,77]. Candan and colleagues explored the effect of selenium on rats with LPS-induced kidney injury. Specifically, selenium treatment resulted in the upregulation of AQP1 expression, which was initially decreased by LPS exposure [39]. Furthermore, Ozden and colleagues demonstrated the protective effect exerted by dexpanthenol in LPS-induced kidney injury. Dexpanthenol is a precursor of vitamin B with proven anti-apoptotic and anti-inflammatory properties [78,79]. Interestingly, in rats exposed to LPS, treatment with dexpanthenol increased AQP2 mRNA expression in kidney tissues through the silent information regulator 1 (SIRT1) signaling pathway [43]. Liu et al. demonstrated that the downregulation of AQP2 protein expression in rats with LPS-induced kidney injury could be attenuated by rhein treatment, an anthraquinone utilized in Chinese medicine with anti-inflammatory, antioxidant, and hepatoprotective properties [42,80]. Pre-treatment with propofol has been demonstrated to protect LPS-exposed rats with sepsis-induced kidney injury from further renal complications, by preventing the downregulation of AQP2 expression [44]. In the case of AQP9, recent studies have demonstrated that inhibition of its expression may present beneficial effects against sepsis progression. Pre-treatment with RG100204, a novel inhibitor of AQP9 expression, has been shown to attenuate cardiac and renal dysfunction and hepatocellular injury caused by CLP-induced sepsis in mice [81]. Interestingly, knockout of the AQP9 gene in LPS-exposed mice has been demonstrated to increase survival rates, reduce the expression of the inflammatory transcription factor NF-kB p65, and protect from LPS-induced oxidative stress [82].
Table 3 lists regulators of AQP expression and their effect on experimental sepsis progression.

5. The Role of Aquaporin Single Nucleotide Polymorphisms in Clinical Sepsis

In recent years, single nucleotide polymorphisms (SNPs) in aquaporin genes have been associated with numerous pathological conditions, presenting great clinical value. Specifically, the 1364 A/C polymorphism in the gene promoter region of AQP5 has been extensively studied in sepsis. An initial study demonstrated that in patients with severe sepsis, 30-day survival was strongly associated with the presence of the 1364 A/C polymorphism. More specifically, 1364 A/C seemed to have a protective role, whereas carriers of the AA genotype were at a higher mortality risk [83]. Furthermore, the 1364 A/C polymorphism was strictly associated with expression levels of AQP5 and the migration of immune cells. AQP5 mRNA expression was significantly greater in septic patients’ blood carrying the AA genotype compared to AC carriers. Additionally, neutrophils from AA donors demonstrated faster and greater migration compared to AC carriers [19]. These results could attribute a protective role of 1364 A/C in the reduction of AQP5 expression, cell migration, and proliferation. The presence of the 1364 A/C polymorphism has been implicated in the development of major adverse kidney events in patients suffering from sepsis. Specifically, AC carriers were found less likely to develop major adverse kidney events within the first 30 days, and furthermore presented significantly lower risk of death within 90 days, compared to patients carrying the AA genotype [84].
The use of AQP5 1364 A/C as a survival prognostic factor has also been examined in ARDS patients. In a cohort of 136 patients suffering from ARDS, individuals carrying the AQP5 AC genotype demonstrated greater 30-day survival rates compared to patients carrying the AA genotype. Results demonstrated that the AQP5 1364 A/C polymorphism could be used as a prognostic factor for 30-day survival in combination with other established predictors [85]. The same investigators demonstrated that in ARDS patients suffering from acute kidney injury (AKI), AQP5 1364 A/C was associated with the patients’ recovery rate. Specifically, individuals carrying the AQP5 AC genotype presented significantly higher AKI recovery rate on day 30, compared to AA carriers [86].
DNA methylation of the AQP5 promoter region is central in the regulation of the gene’s expression. A hypomethylated state of the AQP5 promoter combined with increased binding of the specificity protein 1 (SP1) transcription factor resulted in increased AQP5 expression [87]. Apart from increased AQP5 expression discussed above, increased methylation of C (nt-937), which is located within the promoter region of AQP5 and is a target of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), has been also shown in septic non-survivors compared to septic survivors. Additionally, methylation of the AQP5 promoter site nt-937 could be used as a prognostic tool for 30-day mortality in septic patients [12]. Methylation of the AQP5 promoter was examined in neutrophils, monocytes, and lymphocytes from healthy individuals and septic patients. Carries of the AC genotype exhibited increased methylation in both groups [88].

6. Conclusions

This review focused on describing recent advancements regarding the expression and regulation of aquaporins in sepsis and conditions induced by sepsis, as ARDS and AKI. We examined recently published studies on clinical sepsis and well-established experimental models of sepsis. Many studies have demonstrated the interplay between long non-coding RNAs and micro RNAs in the regulation of aquaporins and how this interaction affects sepsis progression. Furthermore, in recent years a growing number of studies have focused on exploring the effect of novel molecules on the expression of aquaporins during sepsis and their potential as possible therapeutic approaches against sepsis development. AQP1 and AQP5 expression has been found downregulated by numerous investigators in experimental models of sepsis-induced ARDS. Many molecules have been found capable of restoring expression to normal levels, while simultaneously attenuating lung injury progression. AQP3 and AQP4 have been found to promote sepsis development with their suppression proving to be beneficial for the experimental models. Moreover, AQP1 and AQP2 upregulation by various molecules in the setting of sepsis-induced AKI has been shown to suppress the disease’s development. Moreover, AQP9 mRNA expression has been found elevated in human subjects injected with LPS as well as in a cohort of ARDS patients, while recent studies have demonstrated that inhibition of AQP9 expression may protect from sepsis-induced complications. Finally, we explored the effect of SNPs on the expression of aquaporins and their importance as a clinical tool based on recent advancements. Future studies could explore whether molecules with a proven effect on experimental models of sepsis present the same utility in a clinical setting.

Author Contributions

All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kreida, S.; Tornroth-Horsefield, S. Structural insights into aquaporin selectivity and regulation. Curr Opin Struct Biol 2015, 33, 126–134. [Google Scholar] [CrossRef] [PubMed]
  2. Verkman, A.S.; Anderson, M.O.; Papadopoulos, M.C. Aquaporins: important but elusive drug targets. Nat Rev Drug Discov 2014, 13, 259–277. [Google Scholar] [CrossRef] [PubMed]
  3. Agre, P.; King, L.S.; Yasui, M.; Guggino, W.B.; Ottersen, O.P.; Fujiyoshi, Y.; Engel, A.; Nielsen, S. Aquaporin water channels--from atomic structure to clinical medicine. J Physiol 2002, 542, 3–16. [Google Scholar] [CrossRef] [PubMed]
  4. Yasui, M. Molecular mechanisms and drug development in aquaporin water channel diseases: structure and function of aquaporins. J Pharmacol Sci 2004, 96, 260–263. [Google Scholar] [CrossRef] [PubMed]
  5. Markou, A.; Unger, L.; Abir-Awan, M.; Saadallah, A.; Halsey, A.; Balklava, Z.; Conner, M.; Tornroth-Horsefield, S.; Greenhill, S.D.; Conner, A.; et al. Molecular mechanisms governing aquaporin relocalisation. Biochim Biophys Acta Biomembr 2022, 1864, 183853. [Google Scholar] [CrossRef]
  6. Meli, R.; Pirozzi, C.; Pelagalli, A. New Perspectives on the Potential Role of Aquaporins (AQPs) in the Physiology of Inflammation. Front Physiol 2018, 9, 101. [Google Scholar] [CrossRef]
  7. Singer, M.; Deutschman, C.S.; Seymour, C.W.; Shankar-Hari, M.; Annane, D.; Bauer, M.; Bellomo, R.; Bernard, G.R.; Chiche, J.D.; Coopersmith, C.M.; et al. The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA 2016, 315, 801–810. [Google Scholar] [CrossRef] [PubMed]
  8. Rudd, K.E.; Kissoon, N.; Limmathurotsakul, D.; Bory, S.; Mutahunga, B.; Seymour, C.W.; Angus, D.C.; West, T.E. The global burden of sepsis: barriers and potential solutions. Crit Care 2018, 22, 232. [Google Scholar] [CrossRef] [PubMed]
  9. Pierrakos, C.; Velissaris, D.; Bisdorff, M.; Marshall, J.C.; Vincent, J.L. Biomarkers of sepsis: time for a reappraisal. Crit Care 2020, 24, 287. [Google Scholar] [CrossRef]
  10. Vassiliou, A.G.; Maniatis, N.A.; Orfanos, S.E.; Mastora, Z.; Jahaj, E.; Paparountas, T.; Armaganidis, A.; Roussos, C.; Aidinis, V.; Kotanidou, A. Induced expression and functional effects of aquaporin-1 in human leukocytes in sepsis. Crit Care 2013, 17, R199. [Google Scholar] [CrossRef]
  11. Matsushima, A.; Ogura, H.; Koh, T.; Shimazu, T.; Sugimoto, H. Enhanced expression of aquaporin 9 in activated polymorphonuclear leukocytes in patients with systemic inflammatory response syndrome. Shock 2014, 42, 322–326. [Google Scholar] [CrossRef] [PubMed]
  12. Rump, K.; Unterberg, M.; Dahlke, A.; Nowak, H.; Koos, B.; Bergmann, L.; Siffert, W.; Schafer, S.T.; Peters, J.; Adamzik, M.; et al. DNA methylation of a NF-kappaB binding site in the aquaporin 5 promoter impacts on mortality in sepsis. Sci Rep 2019, 9, 18511. [Google Scholar] [CrossRef] [PubMed]
  13. Talwar, S.; Munson, P.J.; Barb, J.; Fiuza, C.; Cintron, A.P.; Logun, C.; Tropea, M.; Khan, S.; Reda, D.; Shelhamer, J.H.; et al. Gene expression profiles of peripheral blood leukocytes after endotoxin challenge in humans. Physiol Genomics 2006, 25, 203–215. [Google Scholar] [CrossRef] [PubMed]
  14. Force, A.D.T.; Ranieri, V.M.; Rubenfeld, G.D.; Thompson, B.T.; Ferguson, N.D.; Caldwell, E.; Fan, E.; Camporota, L.; Slutsky, A.S. Acute respiratory distress syndrome: the Berlin Definition. JAMA 2012, 307, 2526–2533. [Google Scholar] [CrossRef]
  15. Varisco, B.M. The pharmacology of acute lung injury in sepsis. Adv Pharmacol Sci 2011, 2011, 254619. [Google Scholar] [CrossRef] [PubMed]
  16. Xie, Y.; Luo, J.; Hu, W.; Ye, C.; Ren, P.; Wang, Y.; Li, X. Whole Transcriptomic Analysis of Key Genes and Signaling Pathways in Endogenous ARDS. Dis Markers 2022, 2022, 1614208. [Google Scholar] [CrossRef] [PubMed]
  17. Zhu, D.D.; Huang, Y.L.; Guo, S.Y.; Li, N.; Yang, X.W.; Sui, A.R.; Wu, Q.; Zhang, Y.; Kong, Y.; Li, Q.F.; et al. AQP4 Aggravates Cognitive Impairment in Sepsis-Associated Encephalopathy through Inhibiting Na(v) 1.6-Mediated Astrocyte Autophagy. Adv Sci (Weinh) 2023, 10, e2205862. [Google Scholar] [CrossRef] [PubMed]
  18. Fang, Y.; Hu, J.; Wang, Z.; Zong, H.; Zhang, L.; Zhang, R.; Sun, L. LncRNA H19 functions as an Aquaporin 1 competitive endogenous RNA to regulate microRNA-874 expression in LPS sepsis. Biomed Pharmacother 2018, 105, 1183–1191. [Google Scholar] [CrossRef]
  19. Rump, K.; Unterberg, M.; Bergmann, L.; Bankfalvi, A.; Menon, A.; Schafer, S.; Scherag, A.; Bazzi, Z.; Siffert, W.; Peters, J.; et al. AQP5-1364A/C polymorphism and the AQP5 expression influence sepsis survival and immune cell migration: a prospective laboratory and patient study. J Transl Med 2016, 14, 321. [Google Scholar] [CrossRef]
  20. da Silva, I.V.; Cardoso, C.; Martinez-Banaclocha, H.; Casini, A.; Pelegrin, P.; Soveral, G. Aquaporin-3 is involved in NLRP3-inflammasome activation contributing to the setting of inflammatory response. Cell Mol Life Sci 2021, 78, 3073–3085. [Google Scholar] [CrossRef]
  21. Li, B.; Chen, D.; Wang, G.; Dong, C.; Wang, X.; Bai, C. Expression of AQP-1, AQP-3, AQP-4 and AQP-5 in pulmonary tissues of mice with endotoxin-induced acute lung injury. Academic Journal of Second Military Medical University 2008, 29, 131–135. [Google Scholar] [CrossRef]
  22. Hasan, B.; Li, F.S.; Siyit, A.; Tuyghun, E.; Luo, J.H.; Upur, H.; Ablimit, A. Expression of aquaporins in the lungs of mice with acute injury caused by LPS treatment. Respir Physiol Neurobiol 2014, 200, 40–45. [Google Scholar] [CrossRef] [PubMed]
  23. Rump, K.; Brendt, P.; Frey, U.H.; Schafer, S.T.; Siffert, W.; Peters, J.; Adamzik, M. Aquaporin 1 and 5 expression evoked by the beta2 adrenoreceptor agonist terbutaline and lipopolysaccharide in mice and in the human monocytic cell line THP-1 is differentially regulated. Shock 2013, 40, 430–436. [Google Scholar] [CrossRef] [PubMed]
  24. Vassiliou, A.G.; Manitsopoulos, N.; Kardara, M.; Maniatis, N.A.; Orfanos, S.E.; Kotanidou, A. Differential Expression of Aquaporins in Experimental Models of Acute Lung Injury. In Vivo 2017, 31, 885–894. [Google Scholar] [CrossRef] [PubMed]
  25. Hong-Min, F.; Chun-Rong, H.; Rui, Z.; Li-Na, S.; Ya-Jun, W.; Li, L. CGRP 8-37 enhances lipopolysaccharide-induced acute lung injury and regulating aquaporin 1 and 5 expressions in rats. J Physiol Biochem 2016, 73, 381–386. [Google Scholar] [CrossRef] [PubMed]
  26. Guo, R.; Li, Y.; Han, M.; Liu, J.; Sun, Y. Emodin attenuates acute lung injury in Cecal-ligation and puncture rats. Int Immunopharmacol 2020, 85, 106626. [Google Scholar] [CrossRef] [PubMed]
  27. Zhang, J.; Wang, P.; Wu, Y.; Zhu, Y.; Peng, X.Y.; Xiang, X.M.; Xue, M.Y.; Li, Q.H.; Li, J.X.; Yan, Q.G.; et al. Role of AQP3 in the Vascular Leakage of Sepsis and the Protective Effect of Ss-31. J Cardiovasc Pharmacol 2021, 78, 280–287. [Google Scholar] [CrossRef] [PubMed]
  28. Wang, Y.; Zhang, W.; Yu, G.; Liu, Q.; Jin, Y. Cytoprotective effect of aquaporin 1 against lipopolysaccharide-induced apoptosis and inflammation of renal epithelial HK-2 cells. Exp Ther Med 2018, 15, 4243–4252. [Google Scholar] [CrossRef] [PubMed]
  29. Li, B.; Liu, C.; Tang, K.; Dong, X.; Xue, L.; Su, G.; Zhang, W.; Jin, Y. Aquaporin-1 attenuates macrophage-mediated inflammatory responses by inhibiting p38 mitogen-activated protein kinase activation in lipopolysaccharide-induced acute kidney injury. Inflamm Res 2019, 68, 1035–1047. [Google Scholar] [CrossRef]
  30. Rodrigues, C.E.; Sanches, T.R.; Volpini, R.A.; Shimizu, M.H.; Kuriki, P.S.; Camara, N.O.; Seguro, A.C.; Andrade, L. Effects of continuous erythropoietin receptor activator in sepsis-induced acute kidney injury and multi-organ dysfunction. PLoS One 2012, 7, e29893. [Google Scholar] [CrossRef]
  31. Grinevich, V.; Knepper, M.A.; Verbalis, J.; Reyes, I.; Aguilera, G. Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla. Kidney Int 2004, 65, 54–62. [Google Scholar] [CrossRef] [PubMed]
  32. Olesen, E.T.; de Seigneux, S.; Wang, G.; Lutken, S.C.; Frokiaer, J.; Kwon, T.H.; Nielsen, S. Rapid and segmental specific dysregulation of AQP2, S256-pAQP2 and renal sodium transporters in rats with LPS-induced endotoxaemia. Nephrol Dial Transplant 2009, 24, 2338–2349. [Google Scholar] [CrossRef] [PubMed]
  33. Wang, W.; Li, C.; Summer, S.N.; Falk, S.; Wang, W.; Ljubanovic, D.; Schrier, R.W. Role of AQP1 in endotoxemia-induced acute kidney injury. Am J Physiol Renal Physiol 2008, 294, F1473–F1480. [Google Scholar] [CrossRef] [PubMed]
  34. Li, H.; Shi, H.; Gao, M.; Ma, N.; Sun, R. Long non-coding RNA CASC2 improved acute lung injury by regulating miR-144-3p/AQP1 axis to reduce lung epithelial cell apoptosis. Cell Biosci 2018, 8, 15. [Google Scholar] [CrossRef] [PubMed]
  35. Tang, R.; Pei, L.; Bai, T.; Wang, J. Down-regulation of microRNA-126-5p contributes to overexpression of VEGFA in lipopolysaccharide-induced acute lung injury. Biotechnol Lett 2016, 38, 1277–1284. [Google Scholar] [CrossRef] [PubMed]
  36. Wang, X.; Zhou, X.; Xia, X.; Zhang, Y. Estradiol attenuates LPS-induced acute lung injury via induction of aquaporins AQP1 and AQP5. European Journal of Inflammation 2021, 19. [Google Scholar] [CrossRef]
  37. Wang, X.; Zhang, Y.; Zhou, X.; Xia, X.; Teng, W.; Sheng, L.; Ding, J. Soy isoflavone reduces LPS-induced acute lung injury via increasing aquaporin 1 and aquaporin 5 in rats. Open Life Sci 2023, 18, 20220560. [Google Scholar] [CrossRef] [PubMed]
  38. Tao, B.; Liu, L.; Wang, N.; Wang, W.; Jiang, J.; Zhang, J. Effects of hydrogen-rich saline on aquaporin 1, 5 in septic rat lungs. J Surg Res 2016, 202, 291–298. [Google Scholar] [CrossRef] [PubMed]
  39. Candan, B.; Karakuyu, N.F.; Gulle, K.; Sarman, E.; Ulusoy Karatopuk, D. Beneficial Effects of Selenium on Kidney Injury via Nf-Kb and Aquaporin-1 Levels. Biol Trace Elem Res 2023. [Google Scholar] [CrossRef]
  40. Liang, W.; Guo, L.; Liu, T.; Qin, S. MEF2C alleviates acute lung injury in cecal ligation and puncture (CLP)-induced sepsis rats by up-regulating AQP1. Allergol Immunopathol (Madr) 2021, 49, 117–124. [Google Scholar] [CrossRef]
  41. Keskinidou, C.; Lotsios, N.S.; Vassiliou, A.G.; Dimopoulou, I.; Kotanidou, A.; Orfanos, S.E. The Interplay between Aquaporin-1 and the Hypoxia-Inducible Factor 1alpha in a Lipopolysaccharide-Induced Lung Injury Model in Human Pulmonary Microvascular Endothelial Cells. Int J Mol Sci 2022, 23. [Google Scholar] [CrossRef] [PubMed]
  42. Liu, C.; Song, N.; Yao, R.; Zhang, X.; Dong, X.; Feng, L. Rhein Improves Sepsis Rats by Reducing Inflammatory Reaction and Regulating AQP2 Acute Renal Injury. International Journal of Health and Pharmaceutical Medicine 2023, 4, 156–163. [Google Scholar] [CrossRef]
  43. Ozden, E.S.; Asci, H.; Buyukbayram, H.I.; Sevuk, M.A.; Imeci, O.B.; Dogan, H.K.; Ozmen, O. Dexpanthenol protects against lipopolysaccharide-induced acute kidney injury by restoring aquaporin-2 levels via regulation of the silent information regulator 1 signaling pathway. Korean J Anesthesiol 2023, 76, 501–509. [Google Scholar] [CrossRef] [PubMed]
  44. Cui, W.Y.; Tian, A.Y.; Bai, T. Protective effects of propofol on endotoxemia-induced acute kidney injury in rats. Clin Exp Pharmacol Physiol 2011, 38, 747–754. [Google Scholar] [CrossRef] [PubMed]
  45. Zheng, C.; Wu, D.; Shi, S.; Wang, L. miR-34b-5p promotes renal cell inflammation and apoptosis by inhibiting aquaporin-2 in sepsis-induced acute kidney injury. Ren Fail 2021, 43, 291–301. [Google Scholar] [CrossRef]
  46. Wang, J.J.; Kong, H.; Xu, J.; Wang, Y.L.; Wang, H.; Xie, W.P. Fasudil alleviates LPS-induced lung injury by restoring aquaporin 5 expression and inhibiting inflammation in lungs. J Biomed Res 2019, 33, 156–163. [Google Scholar] [CrossRef] [PubMed]
  47. Ba, F.; Zhou, X.; Zhang, Y.; Wu, C.; Xu, S.; Wu, L.; Li, J.; Yin, Y.; Gu, X. Lipoxin A4 ameliorates alveolar fluid clearance disturbance in lipopolysaccharide-induced lung injury via aquaporin 5 and MAPK signaling pathway. J Thorac Dis 2019, 11, 3599–3608. [Google Scholar] [CrossRef]
  48. Zhang, Y.; Chen, M.; Zhang, Y.; Peng, P.; Li, J.; Xin, X. miR-96 and miR-330 overexpressed and targeted AQP5 in lipopolysaccharide-induced rat lung damage of disseminated intravascular coagulation. Blood Coagul Fibrinolysis 2014, 25, 731–737. [Google Scholar] [CrossRef]
  49. Xu, J.; Yang, L.; Dong, L. Tanshinol upregulates the expression of aquaporin 5 in lung tissue of rats with sepsis. Oncol Lett 2018, 16, 3290–3296. [Google Scholar] [CrossRef]
  50. Wang, C.; Liang, G.; Shen, J.; Kong, H.; Wu, D.; Huang, J.; Li, X. Long Non-Coding RNAs as Biomarkers and Therapeutic Targets in Sepsis. Front Immunol 2021, 12, 722004. [Google Scholar] [CrossRef]
  51. Ha, M.; Kim, V.N. Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol 2014, 15, 509–524. [Google Scholar] [CrossRef] [PubMed]
  52. Vasudevan, S. Posttranscriptional upregulation by microRNAs. Wiley Interdiscip Rev RNA 2012, 3, 311–330. [Google Scholar] [CrossRef]
  53. Paul, P.; Chakraborty, A.; Sarkar, D.; Langthasa, M.; Rahman, M.; Bari, M.; Singha, R.S.; Malakar, A.K.; Chakraborty, S. Interplay between miRNAs and human diseases. J Cell Physiol 2018, 233, 2007–2018. [Google Scholar] [CrossRef] [PubMed]
  54. Hashemian, S.M.; Pourhanifeh, M.H.; Fadaei, S.; Velayati, A.A.; Mirzaei, H.; Hamblin, M.R. Non-coding RNAs and Exosomes: Their Role in the Pathogenesis of Sepsis. Mol Ther Nucleic Acids 2020, 21, 51–74. [Google Scholar] [CrossRef] [PubMed]
  55. Zhang, Q.; Zhong, C.; Yan, Q.; Zeng, L.H.; Gao, W.; Duan, S. miR-874: An Important Regulator in Human Diseases. Front Cell Dev Biol 2022, 10, 784968. [Google Scholar] [CrossRef] [PubMed]
  56. Zhou, Y.; Sun, L.; Zhu, M.; Cheng, H. Effects and early diagnostic value of lncRNA H19 on sepsis-induced acute lung injury. Exp Ther Med 2022, 23, 279. [Google Scholar] [CrossRef] [PubMed]
  57. Wang, R.; Zhao, J.; Wei, Q.; Wang, H.; Zhao, C.; Hu, C.; Han, Y.; Hui, Z.; Yang, L.; Dai, Q.; et al. Potential of circulating lncRNA CASC2 as a biomarker in reflecting the inflammatory cytokines, multi-organ dysfunction, disease severity, and mortality in sepsis patients. J Clin Lab Anal 2022, 36, e24569. [Google Scholar] [CrossRef] [PubMed]
  58. Liu, F.; Hu, S.; Zhao, N.; Shao, Q.; Li, Y.; Jiang, R.; Chen, J.; Peng, W.; Qian, K. LncRNA-5657 silencing alleviates sepsis-induced lung injury by suppressing the expression of spinster homology protein 2. Int Immunopharmacol 2020, 88, 106875. [Google Scholar] [CrossRef]
  59. Chaudhry, N.; Duggal, A.K. Sepsis Associated Encephalopathy. Adv Med 2014, 2014, 762320. [Google Scholar] [CrossRef]
  60. Mao, X.; Wu, Y.; Xu, W. miR-126-5p expression in the plasma of patients with sepsis-induced acute lung injury and its correlation with inflammation and immune function. Clin Respir J 2023, 17, 629–637. [Google Scholar] [CrossRef]
  61. Li, X.; Mo, J.; Li, J.; Chen, Y. lncRNA CASC2 inhibits lipopolysaccharide-induced acute lung injury via miR-27b/TAB2 axis. Mol Med Rep 2020, 22, 5181–5190. [Google Scholar] [CrossRef] [PubMed]
  62. Zhu, L.; Shi, D.; Cao, J.; Song, L. LncRNA CASC2 Alleviates Sepsis-induced Acute Lung Injury by Regulating the miR-152-3p/PDK4 Axis. Immunol Invest 2022, 51, 1257–1271. [Google Scholar] [CrossRef] [PubMed]
  63. Zhan, Y.A.; Qiu, X.L.; Wang, X.Z.; Zhao, N.; Qian, K.J. Reducing LncRNA-5657 expression inhibits the brain inflammatory reaction in septic rats. Neural Regen Res 2021, 16, 1288–1293. [Google Scholar] [CrossRef] [PubMed]
  64. Xie, W.; Lu, Q.; Wang, K.; Lu, J.; Gu, X.; Zhu, D.; Liu, F.; Guo, Z. miR-34b-5p inhibition attenuates lung inflammation and apoptosis in an LPS-induced acute lung injury mouse model by targeting progranulin. J Cell Physiol 2018, 233, 6615–6631. [Google Scholar] [CrossRef] [PubMed]
  65. Semeraro, N.; Ammollo, C.T.; Semeraro, F.; Colucci, M. Sepsis-associated disseminated intravascular coagulation and thromboembolic disease. Mediterr J Hematol Infect Dis 2010, 2, e2010024. [Google Scholar] [CrossRef] [PubMed]
  66. Jafarzadeh, A.; Paknahad, M.H.; Nemati, M.; Jafarzadeh, S.; Mahjoubin-Tehran, M.; Rajabi, A.; Shojaie, L.; Mirzaei, H. Dysregulated expression and functions of microRNA-330 in cancers: A potential therapeutic target. Biomed Pharmacother 2022, 146, 112600. [Google Scholar] [CrossRef] [PubMed]
  67. Rahimi, H.R.; Mojarrad, M.; Moghbeli, M. MicroRNA-96: A therapeutic and diagnostic tumor marker. Iran J Basic Med Sci 2022, 25, 3–13. [Google Scholar] [CrossRef] [PubMed]
  68. Hu, X.H.; Situ, H.L.; Chen, J.P.; Yu, R.H. Lipoxin A4 alleviates lung injury in sepsis rats through p38/MAPK signaling pathway. J Biol Regul Homeost Agents 2020, 34, 807–814. [Google Scholar] [CrossRef]
  69. Chiang, N.; Serhan, C.N.; Dahlen, S.E.; Drazen, J.M.; Hay, D.W.; Rovati, G.E.; Shimizu, T.; Yokomizo, T.; Brink, C. The lipoxin receptor ALX: potent ligand-specific and stereoselective actions in vivo. Pharmacol Rev 2006, 58, 463–487. [Google Scholar] [CrossRef]
  70. Serhan, C.N.; Chiang, N.; Van Dyke, T.E. Resolving inflammation: dual anti-inflammatory and pro-resolution lipid mediators. Nat Rev Immunol 2008, 8, 349–361. [Google Scholar] [CrossRef]
  71. Luo, S.; Yang, Y.; Chen, J.; Zhong, Z.; Huang, H.; Zhang, J.; Cui, L. Tanshinol stimulates bone formation and attenuates dexamethasone-induced inhibition of osteogenesis in larval zebrafish. J Orthop Translat 2016, 4, 35–45. [Google Scholar] [CrossRef] [PubMed]
  72. Stompor-Goracy, M. The Health Benefits of Emodin, a Natural Anthraquinone Derived from Rhubarb-A Summary Update. Int J Mol Sci 2021, 22. [Google Scholar] [CrossRef] [PubMed]
  73. Sun, Y.; Sun, L.; Liu, S.; Song, J.; Cheng, J.; Liu, J. Effect of emodin on Aquaporin 5 expression in rats with sepsis-induced acute lung injury. J Tradit Chin Med 2015, 35, 679–684. [Google Scholar] [CrossRef] [PubMed]
  74. Qiu, P.; Liu, Y.; Chen, K.; Dong, Y.; Liu, S.; Zhang, J. Hydrogen-rich saline regulates the polarization and apoptosis of alveolar macrophages and attenuates lung injury via suppression of autophagy in septic rats. Ann Transl Med 2021, 9, 974. [Google Scholar] [CrossRef] [PubMed]
  75. Guo, C.; Wu, T.; Zhu, H.; Gao, L. Aquaporin 4 Blockade Attenuates Acute Lung Injury Through Inhibition of Th17 Cell Proliferation in Mice. Inflammation 2019, 42, 1401–1412. [Google Scholar] [CrossRef]
  76. Hariharan, S.; Dharmaraj, S. Selenium and selenoproteins: it's role in regulation of inflammation. Inflammopharmacology 2020, 28, 667–695. [Google Scholar] [CrossRef] [PubMed]
  77. Kielczykowska, M.; Kocot, J.; Pazdzior, M.; Musik, I. Selenium - a fascinating antioxidant of protective properties. Adv Clin Exp Med 2018, 27, 245–255. [Google Scholar] [CrossRef] [PubMed]
  78. Li-Mei, W.; Jie, T.; Shan-He, W.; Dong-Mei, M.; Peng-Jiu, Y. Anti-inflammatory and Anti-oxidative Effects of Dexpanthenol on Lipopolysaccharide Induced Acute Lung Injury in Mice. Inflammation 2016, 39, 1757–1763. [Google Scholar] [CrossRef] [PubMed]
  79. Shin, J.Y.; Kim, J.; Choi, Y.H.; Kang, N.G.; Lee, S. Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells. Curr Issues Mol Biol 2021, 43, 1361–1373. [Google Scholar] [CrossRef]
  80. Zhou, Y.X.; Xia, W.; Yue, W.; Peng, C.; Rahman, K.; Zhang, H. Rhein: A Review of Pharmacological Activities. Evid Based Complement Alternat Med 2015, 2015, 578107. [Google Scholar] [CrossRef]
  81. Mohammad, S.; O'Riordan, C.E.; Verra, C.; Aimaretti, E.; Alves, G.F.; Dreisch, K.; Evenas, J.; Gena, P.; Tesse, A.; Rutzler, M.; et al. RG100204, A Novel Aquaporin-9 Inhibitor, Reduces Septic Cardiomyopathy and Multiple Organ Failure in Murine Sepsis. Front Immunol 2022, 13, 900906. [Google Scholar] [CrossRef] [PubMed]
  82. Tesse, A.; Gena, P.; Rutzler, M.; Calamita, G. Ablation of Aquaporin-9 Ameliorates the Systemic Inflammatory Response of LPS-Induced Endotoxic Shock in Mouse. Cells 2021, 10. [Google Scholar] [CrossRef] [PubMed]
  83. Adamzik, M.; Frey, U.H.; Mohlenkamp, S.; Scherag, A.; Waydhas, C.; Marggraf, G.; Dammann, M.; Steinmann, J.; Siffert, W.; Peters, J. Aquaporin 5 gene promoter--1364A/C polymorphism associated with 30-day survival in severe sepsis. Anesthesiology 2011, 114, 912–917. [Google Scholar] [CrossRef] [PubMed]
  84. Bergmann, L.; Nowak, H.; Siffert, W.; Peters, J.; Adamzik, M.; Koos, B.; Rahmel, T. Major Adverse Kidney Events Are Associated with the Aquaporin 5 -1364A/C Promoter Polymorphism in Sepsis: A Prospective Validation Study. Cells 2020, 9. [Google Scholar] [CrossRef] [PubMed]
  85. Rahmel, T.; Rump, K.; Peters, J.; Adamzik, M. Aquaporin 5 -1364A/C Promoter Polymorphism Is Associated with Pulmonary Inflammation and Survival in Acute Respiratory Distress Syndrome. Anesthesiology 2019, 130, 404–413. [Google Scholar] [CrossRef] [PubMed]
  86. Rahmel, T.; Nowak, H.; Rump, K.; Siffert, W.; Peters, J.; Adamzik, M. The aquaporin 5 -1364A/C promoter polymorphism impacts on resolution of acute kidney injury in pneumonia evoked ARDS. PLoS One 2018, 13, e0208582. [Google Scholar] [CrossRef] [PubMed]
  87. Nomura, J.; Hisatsune, A.; Miyata, T.; Isohama, Y. The role of CpG methylation in cell type-specific expression of the aquaporin-5 gene. Biochem Biophys Res Commun 2007, 353, 1017–1022. [Google Scholar] [CrossRef]
  88. Rump, K.; Spellenberg, T.; von Busch, A.; Wolf, A.; Ziehe, D.; Thon, P.; Rahmel, T.; Adamzik, M.; Koos, B.; Unterberg, M. AQP5-1364A/C Polymorphism Affects AQP5 Promoter Methylation. Int J Mol Sci 2022, 23. [Google Scholar] [CrossRef]
Table 1. Aquaporin mRNA and protein expression in clinical sepsis.
Table 1. Aquaporin mRNA and protein expression in clinical sepsis.
Aquaporin Findings References
AQP1 mRNA upregulation in leukocytes of ICU septic patients [10]
mRNA downregulation in serum of septic patients [18]
AQP4 Protein upregulation in blood samples of SAE patients [17]
AQP5 mRNA expression elevated in whole blood samples of septic patients carrying the AA genotype of the 1364 A/C SNP compared to AC carriers [19]
mRNA expression in non-surviving septic patients in comparison to survivors [12]
AQP9 mRNA upregulation in healthy humans injected with LPS [13]
mRNA upregulation in ARDS patients [16]
AQP, aquaporin; ARDS, acute respiratory distress syndrome; ICU, intesive care unit; LPS, lipopolysaccharide SAE, sepsis associated encephalopathy; SNP, single nucleotide polymorphism.
Table 2. Aquaporin mRNA and protein expression in experimental models of sepsis.
Table 2. Aquaporin mRNA and protein expression in experimental models of sepsis.
Aquaporin Experimental
Models		 Findings References
AQP1 LPS-exposed mice Protein downregulation in the alveoli [22]
LPS-exposed mice mRNA downregulated in the lung [23,34]
LPS-exposed mice Protein downregulation
In the lungs		 [21,35]
LPS-exposed mice mRNA and protein levels
decreased in lung tissues		 [36]
LPS-exposed rats mRNA and protein levels
decreased in lung tissues		 [25,37,38]
LPS-exposed rats mRNA decreased initially and presented steady increase in kidney tissue,
serum protein increased initially and was downregulated in serum and kidneys		 [29]
LPS-exposed rats Protein levels decreased in the kidneys [39]
CLP-rats mRNA and protein levels
decreased in lung tissues		 [26,40]
LPS-exposed
HPMECs		 mRNA upregulation [41]
LPS-exposed
HK2 cells		 mRNA decrease [28]
LPS-exposed
PMNs		 mRNA and protein upregulation [10]
AQP2
LPS-exposed mice Protein downregulation in kidney [33]
LPS-exposed rats Protein downregulation in kidney [31,32,42]
LPS-exposed rats mRNA downregulation in kidney [43,44]
CLP-rats Protein downregulation in kidney [30]
LPS-exposed
HK2 cells		 mRNA and protein decrease [45]
LPS-exposed
HK2 cells		 mRNA decrease [28]
AQP3
LPS-exposed mice Protein downregulation in kidney [33]
CLP-rats mRNA and protein upregulation in lung [27]
LPS-exposed
PMA-treated monocytes		 mRNA upregulation [20]
AQP4
CLP-rats mRNA upregulation in lung [27]
CLP-rats Protein increases in cortical and
hippocampal tissues		 [17]
AQP5
LPS-exposed mice Protein expression decreased in the alveoli [22]
LPS-exposed mice mRNA and protein levels
decreased in lung tissues		 [24,36,46]
LPS-exposed mice Protein expression decreased in lungs [21,47]
LPS-exposed rats mRNA and protein levels
decreased in lung tissues		 [25,37,38,48]
CLP-rats mRNA and protein levels
decreased in lung tissues		 [26,49]
AQP9
LPS-exposed mice mRNA upregulated in lung tissues [24]
LPS-exposed
human leukocytes		 mRNA upregulation [20]
LPS-exposed
PMA-treated monocytes		 mRNA upregulation [20]
AQP, aquaporin; CLP, cecal ligantion and puncture; HK2, human kidney 2; HPMECs, human pulmonary microvascular endothelial cells; LPS, lipopolysaccharide; PMA, phorbol myristate acetate; PMNs, polymorphonuclear neutrophils.
Table 3. Regulators of aquaporin expression and their effect on experimental sepsis progression.
Table 3. Regulators of aquaporin expression and their effect on experimental sepsis progression.
Aquaporin Experimental
Models		 Findings References
AQP1 LPS-exposed mice Estradiol treatment pre-LPS exposure upregulated AQP1 mRNA and protein levels
reducing oxidative stress and inflammatory responses 		[36]
LPS-exposed rats Pre-treatment with increasing concentrations of soy isoflavone resulted in a dose-dependent upregulation of AQP1 mRNA and protein alleviating pulmonary edema and lung damage [37]
LPS-exposed rats Hydrogen-rich saline reverses AQP1 mRNA and protein downregulation [38]
LPS-exposed rats Selenium treatment resulted in the upregulation of AQP1 protein expression in kidneys [39]
CLP-rats MEF2C treatment attenuated the progress of lung injury, while upregulating AQP1 mRNA and protein expression. [40]
CLP-rats Emodin pre-treatment increased significantly AQP1 mRNA and protein suppressing sepsis-induced pulmonary apoptosis [26]
LPS-exposed HPMECs HIF1A expression silencing in the LPS-induced attenuated AQP1 upregulation and regulated cells’ volume increase [41]
AQP2
LPS-exposed rats Dexpanthenol increased AQP2 mRNA expression in kidney tissues through the SIRT1 signaling pathway [43]
LPS-exposed rats Rhein treatment attenuated downregulation of AQP2 protein expression in kidneys [42]
LPS-exposed rats Propofol pre-treatment protected from further kidney complications by restoring AQP2 mRNA levels [44]
AQP3 CLP-rats Ss-31 treatment resulted in decreased protein expression of AQP3 and pulmonary vascular permeability [27]
AQP4
LPS-exposed mice TGN-020 treatment resulted in downregulation of AQP4, suppression of inflammatory cytokine production, and better survival rates [75]
AQP5 LPS-exposed mice Lipoxin A4 presents a protective role in lung injury by upregulating AQP5 protein
expression in lung tissue		 [47]
LPS-exposed mice Fasudil upregulates AQP5 mRNA and protein levels, while eliminating LPS-induced lung edema and preventing LPS-induced pulmonary inflammation [46]
LPS-exposed mice Estradiol treatment pre-LPS exposure upregulated AQP5 mRNA and protein levels
reducing oxidative stress and inflammatory responses		 [36]
LPS-exposed rats Hydrogen-rich saline reverses AQP5 mRNA and protein downregulation [38]
LPS-exposed rats Pre-treatment with increasing concentrations of soy isoflavone resulted in a dose-dependent upregulation of AQP5 mRNA and protein alleviating pulmonary edema and lung damage [37]
CLP-rats Tanshinol treatment reverses diminished AQP5 mRNA and protein levels, while simultaneously inhibiting inflammatory cytokines and p38 phosphorylation [49]
CLP-rats Emodin pre-treatment increased significantly AQP5 mRNA and protein suppressing sepsis-induced pulmonary apoptosis [26]
AQP9 CLP-mice The novel inhibitor RG100204
demonstrated to present positive effects on renal and cardiac dysfunction		 [81]
AQP, aquaporin; CLP, cecal ligation and puncture; HIF1A, hypoxia-inducible factor 1 alpha; HK2, human kidney 2; HPMEC, human pulmonary microvascular endothelial cells; LPS, lipopolysaccharide; MEF2C, myocyte-specific enhancer factor 2C; SIRT1, silent information regulator 1; Ss-31, elamipretide.
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