Version 1
: Received: 22 December 2023 / Approved: 25 December 2023 / Online: 25 December 2023 (04:48:30 CET)
How to cite:
Tilahun, Y.; Quijada-Pinango, J.; Johnson, F.; Smith, K.; Moore, K.; McCallum, M.; Gipson, T.; Yadav, A.; Wang, Z. What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus. Preprints2023, 2023121796. https://doi.org/10.20944/preprints202312.1796.v1
Tilahun, Y.; Quijada-Pinango, J.; Johnson, F.; Smith, K.; Moore, K.; McCallum, M.; Gipson, T.; Yadav, A.; Wang, Z. What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus. Preprints 2023, 2023121796. https://doi.org/10.20944/preprints202312.1796.v1
Tilahun, Y.; Quijada-Pinango, J.; Johnson, F.; Smith, K.; Moore, K.; McCallum, M.; Gipson, T.; Yadav, A.; Wang, Z. What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus. Preprints2023, 2023121796. https://doi.org/10.20944/preprints202312.1796.v1
APA Style
Tilahun, Y., Quijada-Pinango, J., Johnson, F., Smith, K., Moore, K., McCallum, M., Gipson, T., Yadav, A., & Wang, Z. (2023). What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and <em>Haemonchus contortus</em>. Preprints. https://doi.org/10.20944/preprints202312.1796.v1
Chicago/Turabian Style
Tilahun, Y., Archana Yadav and Zaisen Wang. 2023 "What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and <em>Haemonchus contortus</em>" Preprints. https://doi.org/10.20944/preprints202312.1796.v1
Abstract
Abstract: Assessing infection by a parasite, such as Haemonchus contortus, requires time and experienced personnel. Field-based diagnostics using metagenomic species-specific targeting methods would be beneficial. Here, we examine two helminth control methods, zoledronic acid (ZA), an activator of γ δ T cells, and a neutralizing antibody (AB) against γ δ T cells. We conducted a randomized complete block designed investigation for the effects of Haemonchus contortus (Hc) infection on Alpine wethers (young castrated male goats) to identify if metagenomic methods will provide strong evidence for diagnostics. Different tissues were collected from wethers and the microbiome compositions were identified. The relative proportion of microbial community members in the environments; the richness of the microbial community; and the evenness of distribution in microbial community members were determined. It was hypothesized that numerous parameters that include many data points will provide a more precise means to identify infection or health based on similarity to uninfected control data parameters. We found that samples did not sequence well, when multiplexed with high concentration libraries, giving taxonomic numbers that had residual and unmeasured confounding bias related to analysis or selection that define significance of OTUs in the tissue samples at the phylum or species level.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.