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Exosomes from Adipose Tissue-Derived Stem Cells Induce Proapoptotic Gene Expression in Breast Tumor Cell Line

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Submitted:

04 January 2024

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04 January 2024

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Abstract
Lipofilling is an option for breast reconstruction after tumor resection to avoid the complications of an implant-based reconstruction. Although some concerns exist regarding the oncological safety of a tissue rich in mesenchymal stem cells with their proangiogenic and proliferation-supportive properties, there are also reports that adipose tissue-derived stem cells can exhibit antitumoral properties. We isolated primary adipose tissue-derived stem cells. Both conditioned medium and exosomes were harvested from the cell culture and used to treat the breast cancer cell line MCF-7. Cell viability, cytotoxicity, and gene expression of MCF-7 cells in response to the indirect co-culture were evaluated. MCF-7 cells incubated with exosomes from adipose tissue-derived stem cells show reduced cell viability in comparison to MCF-7 cells incubated with adipose-tissue derived stem cell-conditioned medium. Expression of proapoptotic genes was upregulated, expression of antiapoptotic genes was downregulated. The debate about the oncological safety of autologous fat grafting after tumor resection continues. Here, we show that exosomes from adipose tissue-derived stem cells exhibit some antitumoral properties on breast cancer cell line MCF-7.
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Subject: Biology and Life Sciences  -   Life Sciences

1. Introduction

Breast cancer is the most common malignant tumor in women with an incidence of around 25 % and is responsible for 15 % of all cancer related deaths in women [1]. Whereas the mortality rates of mammary carcinoma are declining due to improved therapeutic options and early detection screening, the incidence is continuously increasing [2,3]. Although non-surgical therapy options including radiotherapy and chemotherapy are available, tumor resection remains the crucial mainstay [4,5] and for later stages of breast cancer mastectomy is often inevitable [6]. This procedure is accompanied with a high degree of psychological strain for the patients leading to a strong desire for breast reconstruction [7]. Until a few decades ago implant-based reconstruction was the gold standard for breast reconstruction [8,9,10,11]. However, using the patients’ own tissue in a more regenerative approach has increasingly gained interest in the last years.
Lipofilling or autologous fat grafting (AFT) is one of the most common procedures in plastic surgery. Adipose tissue can be harvested with minimally invasive methods and is rich in adipose tissue-derived stem cells (ADSCs). The immunomodulatory, angiogenic, and proliferation-supporting properties of ADSCs makes adipose tissue an ideal material for regenerative medicine [12,13,14]. Due to complication with silicone implants AFT has also gained increasing interest as therapy after breast tumor resection and mastectomies in the last years [8]. In contrast to the implant-based reconstruction, the autologous reconstruction prevents the risk of implant failure or foreign body reactions. Additionally, autologous reconstruction leads to a higher patient satisfaction and postoperative quality of life [7]. When reconstruction with autologous tissue alone is not possible, for example in very lean patients, the additional usage of adipose tissue in the implant-based reconstruction has been shown to improve the aesthetic outcome [15,16]. However, there are some concerns regarding the oncological safety of autologous fat grafting. ADSCs may be capable of supporting neoangiogenesis and cell proliferation through paracrine signaling and therefore, ADSCs may contribute to tumor relapse [17]. On the other hand, antitumoral properties of both ADSCs and extracellular vesicles (EVs) from ADSCs have been reported [18,19].
The influence of cells on each other both between the same cells and different cell types often relies on paracrine signaling by secreted particles and molecules [20]. EVs are defined as naturally secreted particles with a lipid bilayer that are not self-replicatory [21]. Several subtypes can be identified that differ in terms of particle size as well as the composition of lipids, proteins and nucleic acids [22]. The most important subtypes are microvesicles and exosomes. Microvesicles are between 100 nm and 1000 nm in diameter and as ectosomes emerge from the outward budding and fission of the plasma membrane. Exosomes (between 30 nm and 100 nm in diameter) are initially formed by endocytosis as endosomes. After combining with a multivesicular body the endosomes are released as exosomes by exocytosis. Exosomes carry the tetraspanin CD63 on the surface. Exosomes have been shown to influence cellular behaviour and might mediate the adaptive immune responses to tumors [23].
Here, we evaluated the effect of exosomes isolated from adipose tissue-derived stem cells on the viability and gene expression of breast tumor cell line MCF-7 in comparison to the conditioned medium (CM).

2. Results

2.1. Exosome detection

Isolated exosomes were evaluated using western blot. After electrophoretic separation and blotting a signal from the mouse anti-human CD63 antibody can be detected between 30 kDa and 50 kDa. No signal is seen for the conditioned medium (Figure 1).

2.2. Cell viability and cytotoxicity

Cell viability was tested using a resazurin assay for MCF-7 treated with conditioned medium and medium supplemented with exosomes in the concentrations 10 µl/cm2, 20 µl/cm2, and 30 µl/cm2. Normal growth medium served as control. In comparison to the conditioned medium, the ES medium shows a concentration dependent negative effect on MCF-7 cell viability. However, in the tested concentrations no significant negative effect in comparison to the control medium can be seen (Figure 2). ADSC-conditioned medium has a positive effect on MCF-7 cell viability. In comparison to the conditioned medium the ES medium shows a significantly reduced cell viability in all tested concentrations on day 2 and day 3.
Additionally, cytotoxicity was tested using a LDH assay for MCF-7 treated with conditioned medium and medium supplemented with exosomes in the concentrations 10 µl/cm2, 20 µl/cm2, 30 µl/cm2, and 35µl/cm2. Normal growth medium served as control. The MCF-7 cells treated with ES medium show an increased LDH release after 24 hours, both in comparison with the conditioned medium and the control medium. LDH release in the conditioned medium was lower than in the control medium, whereas LDH release was higher in all exosome concentrations than in the control medium (Figure 3). However, only the 35 µl/cm2 exosome concentration sample was significantly higher than the control medium.

2.3. Gene expression

Real time-RT-PCR was done to evaluate gene regulation after exosome treatment. Expression of pro-apoptotic genes TP53, Bax, Bad, Casp3, Bak1, and RB1 was generally higher in cells treated with the exosome medium compared to both the cells treated with the conditioned medium and the control cells. Only pro-apoptotic gene Bak1 was downregulated in exosome treated cells compared to the control. For anti-apoptotic gene Bcl-2, angiogenesis promoting genes VEGF-A, PDGF-A, and PDGF-B, and the proliferation marker Ki67 expression was higher in cells treated with the conditioned medium compared to the exosome medium. However, except for PDGF-B, expression of these genes was not downregulated in exosome treated cells in comparison to the control cells (Figure 4).

3. Discussion

Lipofilling is commonly used in plastic surgery for the treatment of soft tissue deficits [24,25,26]. Adipose tissue is rich in mesenchymal stem cells which possess a multipotent differentiation potential and have immunomodulatory, angiogenetic and proliferation supporting properties, making it an ideal biomaterial for regenerative medicine [27,12,13,28,29]. However, these properties might also exert a prooncogenic effect on residual cells after tumor resection. Therefore, AFT is viewed critically for breast reconstruction after mastectomy.[30,31]. On the other hand, anti-tumor properties of mesenchymal stem cells have been reported. Especially exosomes as part of the adaptive immune response to tumors could exert antitumoral properties [19,23].
The gold standard for exosome isolation is ultracentrifugation [32]. Lacking the necessary equipment, we have utilized a method based on magnetic beads. Exosomes were successfully isolated from ADSC-conditioned cell culture medium as confirmed with immunodetection (Figure 1). CD63 is a marker for exosomes although it has also been detected on the surface of other vesicles [33,34]. However, when separated electrophoretically, exosomes show a size range between 30 kDa and 60 kDa [35,36], matching the size of the particles detected with our western blot.
Conditioned medium is rich in cytokines and growth factors. It has been shown that ADSC-conditioned medium can increase proliferation in a mammary carcinoma cell line [37]. This is in accordance with our findings. Compared to control medium, ADCS conditioned medium supported MCF-7 cell proliferation significantly (Figure 2). Additionally, ADCS conditioned medium causes a reduced LDH release in MCF-7 cells, which suggests protective properties of ADSC from cell death (Figure 3). For the cytotoxicity assay, ES medium had the adverse effect. LDH release was higher for all tested exosome concentrations than in the control medium. However, only for the highest concentration these differences were statistically significant. In the viability assay, the effects of the ES medium were unambiguou. For all concentrations the cell viability in ES treated cells was lower than in the cells treated with CM, but only for the highest concentration cell viability was lower compared to the control medium, and this difference was statistically not significant. However, the shown viability decrease is concentration dependent, and it is likely that with higher exosome concentrations a more distinct impact on MCF-7 cell viability would have been observed. This is in accordance with the observation that the only significant LDH release increase was seen with the 35 µl/cm2 concentration which was not tested in the viability assay. Preparation of exosome isolate is material- and time-consuming. The LDH assay showed significant results after 24 hours. Therefore, fewer samples were needed which allowed for the testing of higher exosome concentrations. For the viability assay, new ES medium was needed after every measurement, restricting the examinable concentrations. However, for following studies a higher concentration should be used for the viability assays.
There are several properties a cell needs to acquire to become a tumor cell. Among these hallmarks of cancer are the ability to evade apoptosis as well as sustained angiogenesis [38,39]. Therefore, it is important whether ADSCs support angiogenesis and apoptosis resistance in residual tumor cells at the recipient site after AFT. ADSC CM enhances the gene expression of genes for PDGF and VEGF, which are important for neovascularization, of the proliferation marker Ki67, and the antiapoptotic gene Bcl-2 both in comparison to control medium and ES medium. Antiapoptotic genes on the other hand are downregulated in comparison to the ES medium. In contrast, MCF-7 cells treated with ES medium showed increased gene expression for pro-apoptotic genes compared to both cells treated with control medium and cells treated with ADSC CM. This is accordance with other studies where a pro-apoptotic effect of exosomes isolated from mesenchymal stem cells was shown [40]. In our study, antiapoptotic genes, proliferation marker and angiogenesis-related genes are downregulated in comparison to cells treated with ADSC CM. However, compared with the control medium these markers were upregulated, too for the tested concentration. The strong increase in LDH release observed for the highest exosome concentration supports the hypothesis that a higher exosome concentration could cause a further downregulation of these genes, resulting in increased apoptosis and decreased proliferation and angiogenesis. However, it is also possible that a more distinct gene regulation is unnecessary to explain the pronounced effect observed for the LDH assay. An important factor for the initiation of apoptosis is the ratio between Bcl-2 and Bad. If Bcl-2 is predominant, apoptosis is inhibited; if Bad is predominant apoptosis is induced [41]. This mechanism might explain why small changes in the relative gene expression of these genes after CM or ES medium incubation can cause a profound difference in apoptosis induction observed with the LDH assay.
The growth factors and cytokines found in ADSC CM support cell proliferation, cell survival, and angiogenesis. Therefore, concerns about the oncological safety of AFT after tumor resection are justified. However, in our own studies we didn’t find any evidence that autologous fat gafting increases the risk for cancer recurrence [42]. Here, we confirm these properties for the ADSC secretome. However, in most cases of lipofilling these are desired properties to improve the graft take rate. Actually, efforts are being made to improve transplant survival through enhanced angiogenesis and cell survival. In order to reach this goal lipoaspirate is for example enriched with stem cells or supplemented with platelet rich plasma [43,44]. Nonetheless a risk for tumor recurrence from the ADSC secretome cannot be dismissed. Exosomes isolated from ADSCs however seem to have a concentration-dependent opposite effect in regard to apoptosis, proliferation, and angiogenesis. Maybe the intentional enrichment of lipoaspirate should be enriched with exosomes prior to injection for tissue augmentation should be considered specifically for post-oncologic patients, just like adipose tissue lipoaspirate is currently augmented with growth factors or growth factor producing stem cells for non-oncologic indications.
One limitation of our study is the exosome isolation method without ultracentrifugation. In future studies the exosome concentration that caused a significant increase in LDH increase or even higher concentrations should be tested for their effect on cell viability and gene expression.

4. Materials and Methods

4.1. Cell Culture

ADSCs were isolated as previously described XXX. Briefly, lipoaspirate from patients that underwent liposuction was enzymatically digested with a 0.05 % collagenase solution (Collagenase from C. histolyticum, Sigma Aldrich) at 37 °C for 60 minutes, filtered through a 100-µm-filter and centrifuged at 500 rcf for 5 minutes. The supernatant was discarded and the cell pellet was washed with PBS (Pan Biotech) and seeded into cell culture flasks with αMEM (Pan Biotech) supplemented with 10 % fetal calf serum (FCS) and 1 % of a penicillin/streptomycin solution (growth medium). When reaching subconfluency cells were detached using a trypsin/EDTA solution and seeded in a density of 5000 cells per cm2. ADSCs in passage 3 were used for all experiments. MCF-7 cell line was purchased from CLS Cell Lines Service (Eppelheim, Germany) and cultured in the same growth medium. Seeding density was 30,000 cells per cm2.

4.2. Medium harvesting, exosome isolation and exosome detection

ADSCs were seeded into cell culture flasks. Upon reaching subconfluency the medium was changed to a serum-free medium. The medium was harvested 24 hours later. For exosome isolation the harvested medium underwent serial centrifugation (300 rcf for 10 minutes, 2000 rcf for 20 minutes and 4500 rcf for 45 minutes) to remove cell debris. The supernatant was transferred to a new centrifugation tube after every centrifugation step and filtered through a 0.2 µm filter after the last centrifugation step. Subsequently, the exosomes were preconcentrated using Centricon Plus-70 Centrifugal Filter Units (Merck Millipore) with a Molecular Weight Cut Off of 100 kDa. In this way 75 ml medium collected from 5 T175 cell culture flasks with a combined growth area of 875 cm2 were concentrated to a volume of ca. 350 µl. For the final exosome isolation from the medium concentrate the Pan Human Exosome Isolation Kit (Miltenyi Biotec) based on magnetic beads-coupled antibodies was used according to the manufacturers’ instructions. The final isolation volume was 100 µl. For the experiments, normal growth medium was supplemented with different concentrations of exosome isolate. For standardization, the volume of exosome isolate per treated cell growth area in µl/cm2 was used as unit.
ADSC-conditioned medium (CM) was also harvested after 24-hour incubation in serum free medium and was supplemented with 10 % FCS prior to the experiments.
Isolated exosomes were detected using western blot. For SDS-PAGE the Mini-PROTEAN® Tetra Cell electrophoresis chamber (BioRad, Hercules, CA, USA) and the PageRuler Prestained NIR Protein Ladder (Thermo Fisher Scientific) were used. After electrophoresis, separated vesicles were blotted onto a nitrocellulose membrane (Amersham Protran 0.2 NC, Sigma-Aldrich) using the Mini Trans-Blot® Cell wet blotting system (BioRad). Membranes were incubated with primary antibody (mouse anti-human CD63 antibody (Thermo Scientific)) overnight at 4 °C, and the next day with infrared-labeled secondary antibody IRDye 680CW goat anti-mouse (Li-Cor Biosciences, Lincoln, NE, USA)) for one hour at room temperature. Visualization was performed using the Odyssey® Infrared Imaging System.

4.3. Viability and cytotoxicity assays

MCF-7 cells were seeded into 96-well plates (4.800 Zellen/cm2) and incubated with different concentrations of exosome isolate, with conditioned medium and normal growth medium as control. Viability was evaluated using a resazurin assay on 4 consecutive days where day 0 refers to the measurement prior to treatment start. Cells were incubated with normal growth medium supplemented with 0.07 µM resazurin (Sigma Aldrich) for two hours. Metabolic conversion of resazurin into the fluorescent resorufin was detected using a multiwell plate reader (excitation: 530 nm, emission: 590 nm, Varioskan, Thermo Scientific). Cytotoxicity was evaluated using the LDH Assay (abcam) according to themanufacturers’ instructions.

4.4. RNA isolation and RT-PCR

MCF-7 cells were seeded into 24 well plates (50,000 cells per well) in triplicates and allowed to adhere. Afterwards, the medium was changed to control medium (normal growth medium), conditioned medium (CM) or exosome supplemented (ES) medium (30 µl/cm2). RNA was harvested after 24 hours using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. Isolated RNA was reversely transcribed using the QuantiTect Reverse Transcription Kit (Qiagen). For real-time RT-PCR the DyNAmo HS SYBR Green qPCR Kit (Life Technologies) with the Eco™ Real-Time PCR System (Illumina, San Diego, CA, USA) was used. Primers were designed using Primer3 and were purchased from Eurofins MWG Operon. Primer sequences are listed in Table 1. The relative gene expressions were calculated using the ΔΔCt method XVX181, and normalized to the control using glycerinaldehyd-3-phosphat-dehydrogenase (GAPDH) as the housekeeping gene. The gene expression was evaluated for genes related to angiogenesis and apoptosis.

Author Contributions

“Conceptualization, O.F. and L.P.; methodology, S.V.; software, S.V.; validation, O.F., A.E. and S.K.; formal analysis, A.E.; investigation, O.F., S.V., and A.E.; resources, S.K. and L.P..; data curation, S.V.; writing—original draft preparation, S.V.; writing—review and editing, O.F. and S.K.; visualization, S.V.; supervision, L.P.; project administration, L.P.; funding acquisition, L.P. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki, and approved by the Ethics Committee of the University Hospital Regensburg (08/117).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

Data sharing not applicable

Conflicts of Interest

The authors declare no conflict of interest.

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Preprints 95467 g001
Figure 1. Western Blot image. In the left lane is the the PageRuler Prestained NIR Protein Ladder. In the right lane 15 µl exosome isolate were applied. In the middle lane the same volume of unconcentrated supernatant was used. The protein ladder is labeled with the respective molecular weights in kDa.
Figure 1. Western Blot image. In the left lane is the the PageRuler Prestained NIR Protein Ladder. In the right lane 15 µl exosome isolate were applied. In the middle lane the same volume of unconcentrated supernatant was used. The protein ladder is labeled with the respective molecular weights in kDa.
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Figure 2. Resazurin assay for cell viability evaluation. Mean values and standard derivations are shown. MCF-7 cells treated with ADSC ES medium show a significantly lower cell viability than cells treated with ADSC-CM (* p-value < 0.05).
Figure 2. Resazurin assay for cell viability evaluation. Mean values and standard derivations are shown. MCF-7 cells treated with ADSC ES medium show a significantly lower cell viability than cells treated with ADSC-CM (* p-value < 0.05).
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Figure 3. LDH assay for cytotoxicity detection. Mean values and standard derivations are shown. Both the conditioned medium (CM) and the 35 µl/cm2 exosome supplementation (Ex. 35 µl/cm2) differ highly significant from all other groups (** p-value < 0.01; ***: p-value < 0.001).
Figure 3. LDH assay for cytotoxicity detection. Mean values and standard derivations are shown. Both the conditioned medium (CM) and the 35 µl/cm2 exosome supplementation (Ex. 35 µl/cm2) differ highly significant from all other groups (** p-value < 0.01; ***: p-value < 0.001).
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Figure 4. RT-PCR for gene expression. Gene expression of MCF-7 cells after treatment with ADSC-conditioned medium or medium supplemented with 30 µl/cm2 exosome isolate was tested for genes related to apoptosis, angiogenesis, or proliferation. Generally, expression of pro-apoptotic genes was higher in cells treated with the exosome medium compared to the cells treated with the conditioned medium. For anti-apoptotic gene Bcl-2, angiogenesis promoting genes VEGF-A, PDGF-A, and PDGF-B, and the proliferation marker Ki67 expression was higher in cells treated with the conditioned medium compared to the exosome medium. Mean values and standard derivations are shown.
Figure 4. RT-PCR for gene expression. Gene expression of MCF-7 cells after treatment with ADSC-conditioned medium or medium supplemented with 30 µl/cm2 exosome isolate was tested for genes related to apoptosis, angiogenesis, or proliferation. Generally, expression of pro-apoptotic genes was higher in cells treated with the exosome medium compared to the cells treated with the conditioned medium. For anti-apoptotic gene Bcl-2, angiogenesis promoting genes VEGF-A, PDGF-A, and PDGF-B, and the proliferation marker Ki67 expression was higher in cells treated with the conditioned medium compared to the exosome medium. Mean values and standard derivations are shown.
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Table 1. Sequences of primers.
Table 1. Sequences of primers.
Gene Forward primer sequence Reverse primer sequence
Bad 5‘-TCACAAATCCTCCCCAAGTGG-3‘ 5‘-GAATGCGCCCTAAATCACTGA-3‘
Bak1 data data
Bax 5‘-TTCCGAGTGGCAGCTGAGATGTTT-3‘ 5‘-TGCTGGCAAAGTAGAAGAGGGCAA-3‘
Bcl-2 5‘-ATCGCCCTGTGGATGACTGAG-3‘ 5‘-CAGCCAGGAGAAATCAAACAGAGG-3‘
Casp-3 data data
GAPDH 5‘-GGAGCGAGATCCCTCCAAAAT-3‘ 5‘-GGCTGTTGTCATACTTCTCATGG-3‘
Ki67 data data
PDGF-A 5‘-CCCCTGCCCATTCGGAGGAAGAG-3‘ 5‘-TTGGCCACCTTGACGCTGCGGTG-3‘
PDGF-B 5‘-GATCCGCTCCTTTGATGATC-3‘ 5‘-GTCTCACACTTGCATGCCAG-3‘
RB1 5‘-TTGGATCACAGCGATACAAACTT-3‘ 5‘-AGCGCACGCCAATAAAGACAT-3‘
TP53 5‘-GGAGTATTTGGATGACAGAAAC-3‘ 5‘-GATTACCACTGGAGTCTTC-3‘
VEGF-A 5‘-AACCAGCAGAAAGAGGAAAGAGG-3‘ 5‘-CCAAAAGCAGGTCACTCACTTTG-3‘
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