A five-step sequence was developed to forge 1A (Scheme 1). S_N 2 reaction of methyl 2-aminopyridine-4-carboxylate with 2-chloro-5-nitropyrimidine, followed by nitro reduction (H₂, Raney Ni), afforded intermediate 3, that underwent addition to 1-chloro-4-isocyanato-2-(trifluoromethyl)benzene, accomplishing the formation of intermediate 4. Then hydrolysis of methyl ester of 4, afforded carboxylic acid 5 that was transformed to carboxamide 1A under coupling reagents condition (HATU, DIEA). Finally, base-mediated (NaH, DMF) rearrangement of 1A delivered 1A2 (Figure 3) in high yield. Crystallization 1A2 from mixed solvent of methanol and dichloromethane obtained 1A2a as a solvate with dichloromethane.

Single-crystal X-ray structure analysis reveals that compound 1A2a is a dichloromethane solvate at the stoichiometric ratio 1:1 and crystallizes in the triclinic system space group P1 with lattice parameters of a = 0.98517(7) nm, b = 1.02973(7) nm and c = 1.14712(8) nm). The crystal size is 0.340×0.270×0.080 mm³ (Table 1). The crystal structure analysis of interatomic distances in crystal 1A2a indicates the presence of a set of intramolecular C-F7…H51-C, C-O10…H28-C, C-Cl1…C42-F, C-O10…C41 interaction, one N17…H12-N hydrogen bond and intermolecular C-O10…H48-C interaction formed between the carbonyl group and the solvate molecule (Table 2). By study and comparison their geometrical parameters, it is possible to suggest that all of these interactions are weak, except for N17…H12-N hydrogen bond (Figure 3). View of the pack drawing of 1A2a is shown in Figure 4.

The cytotoxicity of compound 1A, 1A2 on five human cancer cell lines, including A549 (lung), HepG-2 (hepatocellular carcinoma, HCC), HCT116 (colon carcinoma), MDA-MB-231 (breast), PC-3 (prostate) were determined by an MTS method (Table 3). The effect of the two compounds on EGFR kinase was assessed using the EGFR Kinase Enzyme System (Promega) (Table 3). Cytotoxicity results showed that compound 1A displayed good antiproliferative activity in MDA-MB-231 (breast) cells with an IC₅₀ value of 16.18±1.42 μM. However, it was found to be ineffective on other cell lines. To our disappointment, compound 1A2 exerted no potencies in the five cancer cell lines (IC₅₀s＞40 μM). Both compounds show little ability to inhibit the EGFR kinase, with compound 1A2 displayed relatively higher inhibition percentage than compound 1A at 40 μM concentration.

NMR spectra were recorded on Bruker DRX-400 instrument with TMS as internal standard. The Chemical shifts (δ ppm) are calibrated with reference to the deuterated solvent DMSO (δ 2.50 for ¹H NMR and 39.52 for ¹³C NMR); CD₃OD (δ 3.31 for ¹H NMR and 49.00 for ¹³C NMR), and coupling constants (J) are given in Hz. Silica gel column chromatography (200-300 mesh) was performed with various combinations of dichloromethane and methanol as eluent to purify compounds. ESI-MS analysis was determined on API QSTAR Pulsari spectrometer. Infrared spectra were recorded on a FT-IR spectrometer with KBr pellets. Yields refer to purified, dried and spectroscopically (¹H NMR) homogeneous material. All the commercial chemicals were used without further purification.

The crystal data for compound 1A2a was collected at 100(2) K on APEX DUO using Cu Kα radiation (λ = 1.54178Å). Data absorption corrections were applied using Semi-empirical from equivalents methods. This structure was solved using refinement method of full⁃matrix least⁃squares against F² with all non⁃hydrogen atoms anisotropic and hydrogen atoms isotropic. The final R₁ values were 0.0932 with I > 2σ(I). Maximum and minimum transmission factors were 0.73 and 0.29, respectively. 18242 unique reflections were measured giving 4496 independent reflections (R_int = 0.0875). An ORTEP representation of the coordination environment of the compound 1A2a including the atom labeling scheme is shown in Figure 3. The diffraction data is listed in . The selected bond, interatomic distances and torsion angles is listed in Table 2.

The human tumor cell lines A549, HepG2, HCT116, MDA-MB-231, and PC-3 which were obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco's modified Eagle's medium (DMEM) or RMPI-1640 containing 10% heat-inactivated fetal bovine serum (FBS) at 37 ^oC in 5% CO₂, then seeded in 96-well plates at the concentration of 3000-5000 cells per well for 12-24 h when they were growing exponentially. The cells were treated with all compounds in different concentrations in triplicates, and incubated for another 48 h. A 20 μL of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfopheny)-2H-tetrazolium, inner salt (MTS, 1.9 mg/mL) and 100 μL culture medium were added to all wells and stained for 2-4 h at 37^oC. The absorbance was measured with a MULTISKAN FC reader at λ = 492 nm. Data from triplicate experiments were expressed as mean ± SD. The IC₅₀ value of each compound was calculated using Reed and Muench’s method.

The inhibitory percentage against EGFR kinase of compounds 1A, 1A2 at 40 μM was measured using the EGFR Kinase Enzyme System (Promega). Operation steps strictly followed instructions from the manufacturer. EGFR (3 units/reaction), reaction buffer A, MnCl₂, DTT and peptide substrate were added to compound and incubated at 30 °C for 10 min followed by addition of ATP and an hour incubation at 30 °C. ADP-Glo™ Reagent was then added and incubated for another 40 min at 30 °C. A final 30 min incubation was needed after the addition of the Kinase Detection Reagent. Luminescence was measured using FlexStation 3.

To a solution of methyl 2-aminopyridine-4-carboxylate (1) (10.0 g, 65.8 mmol) in DMF (150 mL) was added 2-chloro-5-nitropyrimidine (10.5 g, 65.8 mmol) at 0 ^oC. Then mixture was warmed to room temperature and stirred overnight under nitrogen atmosphere. Upon completion of the reaction as indicated by TLC, the mixture was vacuum-filtered,and the filter cake was purified via silica gel column chromatography with DCM and MeOH as the eluent (10: 1 DCM: MeOH) to afford compound 2 as a yellow solid (13.0 g, 63%). ¹H NMR (400 MHz, DMSO-d₆) δ 11.17 (br s, 1 H), 10.12 (br s, 1 H), 9.85 (s, 2 H), 8.67 (d, J = 7.4 Hz, 1H), 8.04 (s, 1 H), 7.27 (d, J = 7.3 Hz, 1H), 3.95 (s, 3 H); IR (KBr) ν_max (cm^-1): 3430, 3234, 3033, 2958, 2852, 1732, 1671, 1606, 1531, 1418, 1349, 1277, 1116, 867, 765, 648, 532, 429; ESI-MS displays a peak at m/z 276.1, calcd for [M + H]⁺: 276.2282.

To a solution of compound 2 (13.0 g, 41.7 mmol) in MeOH (100 mL) was added Raney Ni (3.0 g). The reaction mixture was degassed and back filled with hydrogen for three times, then attached to a hydrogen source, stirred vigorously at room temperature overnight. The catalyst was removed by filtration and the filtrate was concentrated to give a crude material which was purified by flash column chromatography (silica, 10: 1 DCM: MeOH) to yield compound 3 as a yellow solid (5.2 g, 44.2%). ¹H NMR (400 MHz, CD₃OD) δ 8.77 (d, J = 7.4 Hz, 1H), 8.31 (s, 2 H), 7.72 (d, J = 1.6 Hz, 1 H), 7.32 (dd, J₁ = 7.4 Hz, J₂ = 1.6 Hz, 1 H), 4.00 (s, 3 H); IR (KBr) ν_max (cm^-1): 3414, 3236, 3119, 1723, 1674, 1627, 1583, 1441, 1301, 1204, 996, 831, 756, 721, 669, 553; ESI-MS displays a peak at m/z 246.4, calcd for [M + H]⁺: 246.2453.

To a solution of compound 3 (5.2 g, 18.4 mmol) and Et₃N (3.7 g, 36.8 mmol) in DCM (100 mL) was added 1-chloro-4-isocyanato-2-(trifluoromethyl)benzene (7.0 g, 31.8 mmol) at 0 ^oC. Then the reaction progressed at room temperature overnight. Upon completion the reaction, the mixture was washed with brine (30 mL x 3), dried over Na₂SO₄, and evaporated under vacuum to give a crude material, which was purified via silica gel column chromatography with DCM and MeOH as the eluent (10: 1 DCM: MeOH) to afford compound 4 as a yellow solid (5.0 g, 58%). ¹H NMR (400 MHz, CD₃OD) δ 9.34 (s, 1 H), 9.25-9.10 (m, 1 H), 8.38 (s, 2 H), 8.07 (s, 1 H), 7.84 (d, J = 6.7 Hz, 1 H), 7.75 (dd, J₁ = 8.7 Hz, J₂ = 2.3 Hz, 1 H), 7.58 (d, J = 8.8 Hz, 1 H), 4.06 (s, 3 H); IR (KBr) ν_max (cm^-1): 3387, 3344, 3214, 3120, 2928, 1735, 1679, 1583, 1556, 1435, 1334, 1200, 1131, 902, 836, 759, 722; ESI-MS displays a peak at m/z 467.3, calcd for [M + H]⁺: 467.8090.

To a solution of compound 4 (5.0 g, 10.7 mmol) in MeOH (100 mL) was added 2 N LiOH (10.7 mL, 21.4 mmol). The mixture was stirred at room temperature overnight. After consumption of the starting material, the mixture was acidified with 2 N HCl to pH 6-7, then concentrated to afford compound 5 as a yellow solid, which was used in the next step without further purification. LC-MS retention time 3.907 minute. ESI-MS displays a peak at m/z 453.0, calcd for [M + H]⁺: 452.7745.

A flask was charged with HATU (7.5 g, 19.7 mmol) and dissolved in DCM/DMF (1: 1) (60 mL), followed by immersion in an ice bath at 0 ^oC, then compound 5 (5.9 g, 13.0 mmol), methylamine hydrochloride (3.5 g, 51.8 mmol) and DIEA (10.1 g, 78.1 mmol) were added to the cold solution. The mixture was flushed with nitrogen, progressed at room temperature overnight. Upon completion the reaction, the mixture was concentrated in vacuo, diluted with AcOEt (300 mL), washed with Saturated Na₂CO₃ (30 mL x 2) and Saturated NaCl (50 mL x 3), respectively. The organic phase was dried over Na₂SO₄, concentrated. The crude material was purified by silica gel column chromatography (10: 1 DCM: MeOH) to afford compound 1A as a yellow solid (2.0 g, 33.0%). ¹H NMR (400 MHz, DMSO-d₆) δ 12.47 (s, 1H), 8.75-8.59 (m, J = 4.6 Hz, 1H), 8.43 (d, J = 5.1 Hz, 1H), 8.34 (s, 1H), 8.07 (s, 2H), 7.85 (d, J = 2.4 Hz, 1H), 7.79 (d, J = 8.5 Hz, 1H), 7.62 (dd, J = 8.5, 2.4 Hz, 1H), 7.42 (dd, J = 5.2, 1.4 Hz, 1H), 5.51 (s, 2H), 2.77 (d, J = 4.5 Hz, 3H); ¹³C NMR (101 MHz, DMSO) δ 165.13, 152.77, 151.99, 150.22, 148.57, 143.91, 142.11, 140.15, 139.13, 135.44, 132.17, 129.32 (q, J = 20.5), 128.91 (q, J = 7.4), 127.02 (q, J = 35.3), 124.02, 121.30, 116.44, 110.91, 26.29; IR (KBr) ν_max (cm^-1): 3440, 3310, 3219, 3124, 1635, 1563, 1506, 1428, 1410, 1314, 1283, 1229, 1131, 1030, 943, 897, 824, 704, 657; ESI-MS displays a peak at m/z 466.0, calcd for [M + H]⁺: 466.8242.

A frame-dried round-bottomed flask was charged compound 1A (932 mg, 2.0 mmol, 1.0 equiv) and sodium hydride (NaH) (240 mg, 6.0 mmol, 3.0 equiv, 60% dispersion in mineral oil), cooled to 0 °C, then DMF (15 mL) was added via syringe all at once. The resulting suspension was allowed to warm to room temperature over 5 min. Upon completion of the reaction, as indicated by TLC, water (30 mL) was added to quench the reaction and extracted with DCM (3 x 30 mL). The combined organic phase was dried over anhydrous Na₂SO₄ filtered, evaporated. The crude material was purified by silica gel column chromatography (10: 1 DCM: MeOH) to afford compound 1A2 as a brown solid (755 mg, 81%). ¹H NMR (400 MHz, DMSO-d₆) δ 9.17 (s, 1H), 8.76 (d, J = 4.4 Hz, 1H), 8.27 (s, 2H), 8.20 (s, 2H), 7.93 (d, J = 6.6 Hz, 1H), 7.81 (br s, 1H), 7.50 (br s, 1H), 6.76 (d, J = 6.5 Hz, 1H), 5.99 (s, 2H), 2.78 (d, J = 4.4 Hz, 3H); ¹³C NMR (101 MHz, DMSO-d₆) δ 164.60, 159.90, 158.45, 148.22, 143.96, 142.96, 142.77, 140.69, 138.67, 131.46, 126.31 (q, J = 120.6), 124.32, 122.67, 121.60, 121.26, 117.49, 116.62 (q, J = 23.7), 105.72, 26.35; ESI-MS displays a peak at m/z 466.0, calcd for [M + H]⁺: 466.8242; IR (KBr) ν_max (cm^-1): 3433, 3350, 3226, 1680, 1663, 1561, 1482, 1439, 1307, 1235, 1133, 1038, 827, 741, 683, 663, 606, 584, 504.

Crystallization 1A2 from mixed solvent of methanol and dichloromethane afforded 1A2a as a dichloromethane solvate at the stoichiometric ratio 1:1 as a brown triclinic crystal. Crystal data. C₁₉H₁₅ClF₃N₇O₂•CH₂Cl₂ 1A2a, M = 550.76, triclinic, space group P1, a = 9.8517(7) Å, b = 10.2973(7) Å, c = 11.4712(8) Å, α = 86.802(3)°, β = 81.486(3)°, γ = 84.921(3)°, V = 1145.29(14) ų, Z = 2, D_calc = 1.597 g/cm³, μ(CuK_α) = 4.164 mm^-1, T = 100.(2) K, F(000) = 560, crystal size = 0.340 x 0.270 x 0.080 mm³, intensities of 18242 reflections (4496 independent, R_int = 0.0875).

The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Figure S2: General Information ; Figure S2: ¹H NMR spectrum (400 MHz) of compound 2 in DMSO-d₆; Figure S3: ¹H NMR spectrum (400 MHz) of compound 3/4 in CD₃OD; Figure S4: ¹H/¹³C NMR spectrum (400 MHz) of compound 1A in DMSO-d₆; Figure S5: ¹H NMR spectrum (400 MHz) of compound 1A2 in DMSO-d₆; Figure S6: IR spectrum of 2/3; Figure S7: IR spectrum of 4/1A2; Figure S8: IR/ ESI-MS spectrum of 1A; Figure S9: ESI-MS spectrum of 1A2.