Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile

Version 1 : Received: 24 January 2024 / Approved: 25 January 2024 / Online: 25 January 2024 (14:08:37 CET)

A peer-reviewed article of this Preprint also exists.

Acosta-Cárdenas, J.; Jiménez-García, L.F.; Cruz-Gómez, S. de J.; Mendoza-von der Borch, A.P.; Segura-Valdez, M. de L. Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile. International Journal of Molecular Sciences 2024, 25, 5281, doi:10.3390/ijms25105281. Acosta-Cárdenas, J.; Jiménez-García, L.F.; Cruz-Gómez, S. de J.; Mendoza-von der Borch, A.P.; Segura-Valdez, M. de L. Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile. International Journal of Molecular Sciences 2024, 25, 5281, doi:10.3390/ijms25105281.

Abstract

Nuclear speckles are compartments enriched in splicing factors present in the nucleoplasm of mammalian cells. Their morphology is linked to the transcriptional and splicing activities of the cell through a recruitment mechanism. Speckles have been studied mostly in mammalian culture cells, and few studies are devoted to speckles in tissue cells. In mammals, speckles are present in different tissues and their morphology also depends on the hormonal cycle in rats. In the present work, we explore whether a similar situation is also present in non-mammalian tissue cells dur-ing the reproductive cycle. We studied the speckled pattern in several tissues of a viviparous rep-tile, the lizard Sceloporous torquatus, during two different stages of reproduction. We used immu-nofluorescence staining against splicing factors in hepatocytes and oviduct epithelium cells and fluorescence and confocal microscopy. The distribution of splicing factors in the nucleoplasm of oviductal cells and hepatocytes coincides with the nuclear speckled pattern described in mam-mals. In addition, the morphology of speckles varies in oviduct cells at the two stages of the re-productive cycle analyzed, paralleling the phenomenon observed in the rat. The results show that the morphology of speckles in reptile cells depends upon the reproductive stage as it occurs in mammals.

Keywords

cell nucleus; nuclear speckles; reptile; splicing factors; SR proteins; ribonucleoproteins

Subject

Biology and Life Sciences, Cell and Developmental Biology

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