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Natural Antimicrobials in Dairy Products: Benefits, Challenges, and Future Trends

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03 April 2024

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04 April 2024

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Abstract
This review delves into using natural antimicrobials in the dairy industry and examines various sources of these compounds, including microbial, plant, and animal sources. It highlights dairy products' most commonly used natural antimicrobials, such as nisin, lysozyme, lactoferrin, chitosan, and essential oils. It discusses the mechanisms by which they inhibit microbial growth and improve the shelf life of dairy products. Moreover, the review explores encapsulation technology as a promising approach for increasing the viability of natural antimicrobials. It discusses how encapsulation technology can protect these compounds from environmental factors that can reduce their effectiveness, such as pH, temperature, and oxygen exposure. The review also highlights the different materials that can be used for encapsulation, such as alginate, chitosan, and starch, and discusses their properties and suitability for encapsulating different natural antimicrobials. Finally, the review examines the benefits and challenges of using natural antimicrobials in dairy products. While natural antimicrobials offer several advantages, including improved safety, quality, and sensory properties of dairy products, it's crucial to be aware of the challenges associated with their use, such as potential allergenicity, regulatory requirements, and consumer perception. The review concludes by emphasizing the need for further research to identify and develop effective and safe natural antimicrobials for the dairy industry to ensure the quality and safety of dairy products for consumers.
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Subject: Chemistry and Materials Science  -   Other

1. Introduction

Despite the advances in health control measures in the food production industry [1], data from the World Health Organization (WHO) suggests that about 600 million people worldwide suffer from foodborne diseases (FADs), resulting in 420,000 deaths annually. Dairy products account for approximately 14% of these cases [2]. This is because dairy products can carry significant pathogens such as Listeria monocytogenes, Salmonella spp, Staphylococcus aureus, and Escherichia coli [3].
One of the biggest hurdles the food industry faces is preventing the decay of food products caused by microbial activity. This deterioration leads to the loss of nutrients, flavor, and texture, making the products unsuitable for commercialization [4]. As a result, the dairy sector has prioritized maintaining microbiological quality to ensure food safety and prevent economic losses. This has led to increased use of preservatives with antimicrobial properties in food formulations.
Benzoates and sorbates are commonly used as food preservatives and have a history of safe use [5]. However, some studies suggest these substances may have allergenic potential in sensitive individuals [6]. Furthermore, they can form toxic and carcinogenic by-products [7], which is a cause for concern among consumers. As a result, there is a growing demand for foods free from artificial preservatives, which puts pressure on the industry to focus on producing "clean label" products [8].
In this scenario, natural antimicrobial compounds are a promising alternative for conserving and increasing the shelf life of foods. They can be classified according to the source (microbial, plant, and animal), biological function, and biosynthesis [9]. Bacteriocins are the most widely used antimicrobials from microbial sources, produced mainly by gram-positive bacteria, including lactic acid bacteria (LAB) [10]. One example is nisin, which can be added directly to products in its purified form or produced in-situ by probiotics, with efficacy described mainly in milk [11], yogurt [12], and cheeses [11,13]. Biopreservatives of animal origin have also been used in these products, such as lysozyme, lactoferrin, and chitosan [14,15,16,17]. These substances generally can directly inhibit microorganisms and biofilm formation and catalyze reactions that produce antimicrobial compounds [18].
Several studies have evaluated using plant extracts to replace conventional preservatives in dairy products. This has resulted in an improvement in both the quality and shelf life of these products, as well as an enhancement of their sensorial aspects [19,20,21,22]. The use of essential oils (EOs) has also been studied in inhibiting the growth of microorganisms within dairy matrices [23,24]. These studies have shown further benefits, such as improving the sensorial and nutritional aspects of formulations containing lactic acid [24]. Additionally, essential oils offer functional properties such as anti-inflammatory, antioxidant, antispasmodic, diuretic, and tonic action [25].
Although natural antimicrobials have been successfully used in the food industry, some factors intrinsic and extrinsic to the food can reduce their effectiveness [9,26]. Encapsulating these compounds has emerged as a promising technology to overcome this issue. It involves isolating bioactive substances in a protective coating, which improves their viability, stability, and solubility [27]. Studies have shown that capsules made from different materials can preserve biopreservatives and deliver them to the target location [27,28,29]. This approach can help maintain dairy products' physicochemical and sensory properties [29].
The dairy industry and researchers are constantly exploring the potential of natural antimicrobials from different sources. In this regard, a review has been conducted to highlight the effects of applying such antimicrobials to dairy products. This review aimed to provide a deeper understanding of the potential benefits and challenges of using natural antimicrobials in the dairy industry. The review also explores encapsulation as a promising alternative for increasing the viability of these compounds. This will help identify the most effective and safe natural antimicrobial options for dairy products, ensuring the quality and safety of the products for consumers.

2. Methodology

To gather relevant data for a review on natural antimicrobials in dairy products, a comprehensive literature search was conducted using three prominent academic databases: Science Direct, PubMed, and Scopus. The initial search used "natural antimicrobials" and "dairy products". Subsequently, additional search terms, including "bacteriocins," "antimicrobial enzymes," "plant extracts," "essential oils," "milk," "fermented milk," "yogurt," "cheese," and "dairy drink," were also used to refine the search. Priority was given to articles published within the last five years to ensure that the most recent research was included. However, specific older articles which contained significant discussions relevant to the review were also included in the search. In addition, Table 1 summarizes the studies that used antimicrobials in dairy products and their main findings.

3. Bacteriocins

Bacteriocins are antimicrobial peptides synthesized mainly by Lactic Acid Bacteria (LABs), which can be added to food in purified or semi-purified form, or produced in-situ by a starter culture [26]. These compounds can inhibit pathogenic and spoilage microorganisms, without altering the physical-chemical characteristics of the food, in addition to being easily digested by the human gastrointestinal tract [30]. However, the use of bacteriocins as food additives is still limited for several reasons, such as low effectiveness in eliminating specific pathogens or the high cost of large-scale application [31].

3.1. Nisina

Despite its limited effect on Gram-negative bacteria, molds and yeasts, Nisin is widely used in the dairy industry due to its strong action against important Gram-positive bacteria, such as Listeria. monocytogenes and Staphylococcus aureus [32]. Its antimicrobial activity is based on the mechanism of binding to the precursor molecule of the cell wall of the target microorganism, with consequent inhibition of its biosynthesis [33].
Felício et al. [34] evaluated the effects of this bacteriocin on Staphylococcus aureus counts in-vitro and situ in Minas Frescal cheese, noting that in vitr, nisin delayed the lag phase. On the other hand, in the in-situ evaluation, the concentration of 500 IU·mL−1 significantly (p>0.05) reduced the count of this microorganism in the cheese clot and serum. Meshref et al., [35] obtained similar results when studying the effect of nisin against Staphylococcus aureus in kareish cheese. The authors observed significant log reductions after 4 weeks of storage.
Ning et al. [36] investigated the antibacterial activity of nisin synergistically with sucralose laurate (SL) in dairy beverages. Through alkaline phosphatase and peptidoglycan assays, fluorescence spectroscopy, and flow cytometry analysis, the authors observed that these associated compounds block the synthesis of peptidoglycan, damaging the integrity of the cell wall of S. aureus, and inhibiting the activities of Na—ATPase in the cell membrane.
Nisin has variants, A and Z, with nisin A being the most used in the industry as it is a safe and effective preservative when added within recommended limits. Oshima et al., [37] analyzed the effectiveness of nisin A in controlling Bacillus thuringiensis, Bacillus cereus, and Paenibacillus jamilae in a dairy-based dessert, demonstrating that 80 IU g1 and 120 IU g1 of this preservative was able to inhibit the growth of these bacteria in dessert puddings with 5% and 7.5% fat, respectively. Efficiency against spore-forming pathogens, for example Bacillus cereus, is a significant result as it can be aligned with the reduced need to expose the product to high temperatures for an extended period during heat treatment, thus ensuring better preservation of functional characteristics, nutritional, and sensorial of the products.
Although the properties of Nisin Z are still little studied, this variant also demonstrated excellent preservative potential and high stability in dairy products [12]. Murat et al. [11] reported that strains of Lactococcus lactis subsp. lactis have high activity in the synthesis of nisin Z in fluid milk and cheeses, whose purified form was moderately inhibitory on Gram (-) bacteria and stable at temperatures between -20°C and 4°C for 6 weeks. Furthermore, nisin Z extended the shelf life of UHT milk at 30°C by up to 6 weeks and was susceptible to pepsin (92%), lipase (89%), and catalase (84%) at 37°C for 60 min.
Although the numerous advantages of using this bacteriocin have been proven, some factors may affect its effectiveness in dairy products [32]. For example, Modugno et al. [38] report that dairy products with pH close to neutrality (pH>6) can limit their efficiency due to undesirable structural variations, favoring β-folding. Van Tassel et al. [13] proved this statement through the incorporation of nisin and ferrulic acid, isolated or combined, in fresh Hispanic cheese and demonstrated that 4 mg/g of ferrulic acid was significantly more inhibitory than Nisin Z against Listeria innocua and Listeria monocytogenes. Fresh cheeses have the characteristic of having a pH close to neutrality as there is no addition of LABs or maturation processes. Because of this, the use of Nisin Z may have a limited effect on these products, although data in the literature seems contradictory regarding the best pH value for Nisin Z to act [11,13,38,39].
The interaction of nisin with compounds in the food matrix is another limiting factor for its application [12]. Wee et al. [12] characterized and quantified the degradation products of nisin A and Z by HPLC, suggesting that fruit flavoring substances added to yogurt can promote the oxidation of nisin Z, reducing its effectiveness.
Furthermore, microbial resistance to nisin has already been described in cheese by Caprini et al. [39], who added nisin at concentrations of 100 or 500 IU.mL to Minas Frescal cheese but did not observe a reduction in Staphylococcus aureus counts. The authors reinforce the theory that the resistance of certain microorganisms to nisin may vary geographically since different effects were observed in other regions [39]. However, this result may highlight the low effectiveness of nisin in products with a pH close to neutrality [13,38].
Although promising, there is a lack of consensus on some aspects that help to understand the data. For example, in some cases, beneficial effects are reported in fluid milk [11], and at other times, inefficiency is reported at a pH close to neutrality [13,38,39]. In general, interference with antimicrobial action may be more related to the composition of the matrix than its pH. For example, the action of nisin is limited due to its interaction with the casein micelle [40] and fat globules [41], which are present in a more concentrated form in cheese. Furthermore, the presence of divalent cations that block the access of nisin to the membrane of pathogens is another limiting factor [42] and is little discussed in the articles.

3.2. Natamycin

Natamycin is a potent polyene-macrolide antifungal produced by strains of Streptomyces natalenses [43] and recognized as safe (GRAS) by the Food and Drug Administration (FDA) in doses of up to 0.3 mg kg-1 [44]. It is generally used in the dairy industry to preserve cheeses as it presents excellent activity against surface-contaminating fungi and does not modify the sensorial characteristics of the product [43]. Several studies report the effectiveness of Natamycin in inhibiting the growth of Penicillium sp [45,46], Aspergillus sp [47,48], and Cladosporium sp [45,49], with most fungi and yeasts being inhibited by 0.5-6 μg /ml and 1.0 - 5.0 μg /ml of this compound, respectively [50].
Research into the application of Natamycin in dairy products is mainly limited to the conservation of cheese, whether in-situ addition or in the production of edible films and packaging [51,52]. In this scenario, the antifungal activity of Natamycin alone (5, 10, and 20 ppm) and combined at 10 ppm with potassium sorbate (1%) was investigated by Ombarak and Shelaby et al. [51] in Tallaga cheese stored at 4°C for 30 days, demonstrating inhibitory solid activity against fungi at all concentrations studied, including in synergism with potassium sorbate.
González -Forte, Amalv & Bertola [45] demonstrated that Natamycin in a cornstarch coating showed an inhibitory effect on the surface of semi-hard cheeses against fungi isolated from dairy factories including Penicillium spp. In a similar study, Küçük et al. [52] developed alginate and zein films containing Natamycin (100, 200, 500, 1,000, 2,000, and 4,000 ppm) for kosher cheeses, finding that the inhibitory activities of the coatings against Aspergillus niger and Penicillium camemberti increased as the natamycin concentration increased. Torrijos et al. [46] evaluated this compound's in-vitro antifungal activity against Penicillium sp. by spray treatment and in hydroxyethylcellulose film on Mozzarella. They observed that concentrations between 1.6 and 3.1 μg/mL of Natamycin promoted a reduction at 5.28 Log CFU/g and 4.15 Log CFU/g, respectively.
Finally, the antifungal activity of Natamycin was also described in low-density polyethylene (LDPE) packaging by Anari et al. [53]. The modified LDPE with acrylic acid at different UV exposure times (0-10 min) and applied Natamycin to be covalently linked to the pendant functional groups, concluding that films treated for 6 minutes control the growth of Rhodotorula mucilaginosa and Candida parapsilosis and extend the shelf life of fermented milk by 23 days.
Natamycin is widely used in dairy factories, whether by immersion, surface application, or spraying. Studies report very positively its effectiveness against important molds and yeasts in foods despite not being many innovations. In this sense, the biggest trend is its use in packaging and active films, which can become a promising field for studies, especially when considering long-storage dairy products.

3.3. Reuterine

3-hydroxy-propionaldehyde (3-HPA) or reuterin is an antimicrobial naturally produced from glycerol by Lactobacillus reuteri, with inhibitory activity against a broad spectrum of Gram-positive and Gram-negative bacteria, protozoa, molds, and yeasts [54]. It is also recognized as Generally Recognized As Safe (GRAS) and Qualified Presumption of Safety (QPS) in the USA and Europe, respectively [55], being capable of inducing oxidative stress in microorganisms when reacting with thiol groups [56].
Escherichia coli is a common pathogen in cheeses, especially soft and fresh ones [57]. Severe cases of infection with this bacteria can lead to acute gastrointestinal conditions, in addition to kidney failure, hemolytic-uremic syndrome (HUS), and death. Al-Nabuls et al. [58] investigated the survival of this pathogen in salty white cheeses with the addition of Lactobacillus reuteri at different temperatures (10 and 15ºC) and NaCl concentrations (10% and 15% w/w). The study revealed that the presence of L. reuteri in brine cheese at all tested concentrations significantly reduced E. coli O157:H7 counts after 28 days of storage. Similar results were described by Langa et al. [59], who evaluated the protective effect of this antimicrobial produced by Lactobacillus reuteri INIA P572 and 100mM glycerol against Listeria monocytogenes and Escherichia coli O157:H7 during the manufacture and ripening of semi-hard cheese, reporting inhibition of the growth of both after 30 days.
The inhibitory activity of reuterin is also effective against contaminating fungi and was described in yogurts by Vimont et al. [54] and Pilote-Fortin et al. [60]. Vimont et al. [54] quantified the minimum inhibitory activity (MIC) and minimum fungicidal activity (MFC) of reuterin against a representative panel of contaminating fungi and yeasts in yogurt. As a result, there was a fungistatic and fungicidal effect, eliminating around 99.9% of the microorganisms tested at concentrations equal to or lower than 15.6 mM. Pilote- Fortin et al. [60] evaluated the stability and antimicrobial efficacy of this compound at 10 mM added to milk before processing stirred yogurt, finding total inhibitory activity against Rhodotorula mucilaginosa and Mucor racemosus in 1 week and reduction of Aspergillus niger in 3 logarithmic cycles for 4 weeks.
Although the results show efficiency against a wide group of microorganisms, the metrics for evaluating efficiency vary depending on the manufacturing methodology. LABs have the characteristic of using lactose as a substrate, the same used by some Gran (-) bacteria, for example. In this sense, there is a more significant shortage of nutrients. This directly affects the growth of other microorganisms, without knowing precisely the real effect of reuterin against specific pathogens. However, its contribution is evident [58,59]. Using isolated reuterin is a more accurate alternative to verify its efficiency, presenting more significant results against microorganisms [60].

4. Antimicrobial Compounds from Animal Sources

4.1. Lysozyme

Lysozyme is an important antimicrobial found in animal sources, particularly in dairy products like cheese, where it binds easily to casein micelles [61]. It occurs naturally in milk [15] and acts by breaking the β-1,4 bonds between N-acetyl-d-glucosamine and N-acetylmuramic acid residues in the peptidoglycans present in bacterial cell walls [18]. It is especially effective against Gram-positive bacteria. Additionally, it has been found to have antioxidant properties by reducing the formation of reactive oxygen species (ROS) [62].
Cosentino et al. [63] conducted a study on lysozyme in milk. They reported that it has antimicrobial activity against Bacillus megaterium, Bacillus mojavensis, Clavibacter michiganensis, Clostridium tyrobutyricum, Xanthomonas campestris, and Escherichia coli. This activity is similar to synthetic antibiotics and is unaffected by heat treatment. The inhibitory action of lysozyme was also studied by D' Incecco et al. [64] in 16 hard cheeses made with raw milk. The study demonstrated that cheeses with lysozyme had a lower cultivable microbial count and more DNA from lysed bacterial cells. Furthermore, lysozyme caused inhibition of Lactobacillus delbrueckii and Lactobacillus fermentum at the end of 9 and 16 months, respectively.
Ozturkoglu-Budak et al. [65] conducted a study on donkey milk and extracted lysozyme and lactoferrin using FPLC. They applied this extraction to Kashar cheese and found that adding these antimicrobial proteins resulted in cheeses with lower microbial loads. These proteins also helped maintain titratable acidity, increased dry matter content and hardness. They resulted in higher scores for sensory attributes such as appearance, texture, and flavor throughout the maturation period.
Mehyar et al. [66] conducted a study on Halloumi cheese to investigate the effects of chitosan and lysozyme coatings on its microbial quality, shelf life, and sensory properties. The cheese was stored at 3°C and 25°C in brines of 5% and 10% w/w NaCl. The results showed that lysozyme coatings were effective in reducing lactic acid bacteria, psychrotrophic bacteria, anaerobes, molds, and yeasts in cheese salted in a saline solution of up to 15% NaCl. This reduction did not affect the cheese's sensory properties in any way. In another study, Wang et al. [16] concluded that films with immobilized lysozyme exhibited excellent storage stability and resistance to pH and temperature. The films also effectively reduced Staphylococcus aureus in milk at 4°C and 25°C. Furthermore, in a recent study by Awad et al. [17], hydrolyzed peptides from egg white lysozyme showed better antioxidant, sensory, and microbiological effects in yogurt compared to native lysozyme at the same concentration in 28 days of storage.
In addition to reducing bacteria, lysozyme improves sensory characteristics [65,66], particularly texture and storage stability. This may be due to its binding ability to casein micelles [61]. Furthermore, many studies evaluate its use in packaging. Finally, hydrolyzed lysozyme appears to favor the formation of peptides with bioactive properties, increasing the functionality of dairy products and presenting greater antimicrobial activity [17].

4.2. Lactoferrin

Lactoferrin is a glycoprotein found in mammary gland secretions (comprising 10–20% of total milk proteins) and other exocrine fluids [67]. Its bacteriostatic and bactericidal activity is related to its ability to bind iron, a substrate for several pathogens such as Salmonella spp. and L. monocytogenes [68]. Due to its preservative effect and high nutritional value, it has been widely used in dairy products, especially cheese processing [69,70].
Caputo et al. [71] conducted a study to test the effectiveness of bovine lactoferrin hydrolyzed by pepsin (LFH) as a biopreservative against pigmenting pseudomonas psychrotrophic bacteria, which causes spoilage and blue discoloration of Mozzarella cheese. The results showed that LFH prevented the growth of Pseudomonas fluorescens, and no changes were observed in the casein and pigmentation profiles of the cheese samples inoculated with LFH during 14 days of refrigeration. In addition, the count of P. fluorescens was significantly lower compared to the control cheese from day 5 onwards. The pigment leucoindigoidin was only detected in cheese samples without LFH [71]. Also, Biernbaum et al. [72] tested the minimum inhibitory concentration of lactoferrin in raw milk against Salmonella enterica and Escherichia coli O157:H7. The study found that concentrations equal to or greater than 14.06 mg/mL and 112.5 mg/mL of lactoferrin significantly reduced the count of E. coli O157:H7 and S. enterica, respectively.
A recent study conducted by Adnan et al. [73] aimed to determine the effects of different dosages of lactoferrin (5, 10, 15, 20, and 0 mg/100 g) on the microbiological, proximate, antioxidant, and sensory properties of Cheddar cheese. The study revealed that lactoferrin effectively reduced the total viable bacteria count significantly in supplements of 15 and 20mg/100g after 45 days of maturation. Additionally, all dosages increased cheese's antioxidant capacity without affecting its fatty acid content, proximate composition, color, flavor, and texture scores.
It is worth noting that the compound's effectiveness in killing bacteria in cheeses can also be influenced by microbial resistance and various factors such as salt concentration, pH levels, humidity, and product storage temperature [74]. For instance, a study conducted by Hassan et al. [75] investigated lactoferrin treatment's impact on E. coli and S. aureus strains found in Kareish, Domiati, and Tallaga cheeses obtained from different markets and restaurants. The findings indicated that all lactoferrin treatments affected the survival of E. coli in Kareish and Domiati cheese. However, only 20% of the compound reduced S. aureus counts in Kareish cheese, and neither concentration significantly inhibited both pathogens in Tallaga cheese.
It is worth noting that although lactoferrin is effective against microbes, some heat treatments can reduce its efficacy [76]. For instance, Goulding et al. [76] found that temperatures ranging from 72-95°C can permanently alter the physical and chemical properties of bovine lactoferrin, making it unsuitable for use in dairy products that undergo heat treatments at higher temperatures, such as baked cheese production at 72°C.

4.3. Chitosan

Among the most abundant and well-known natural biopolymers, chitosan, extracted from insects, fungi, and crustaceans, stands out as an amino polysaccharide with excellent preservative properties. The food, medical, and pharmaceutical industries commonly use its purified form and derivatives [77]. Chitosan is estimated to have a global market share of US$10.88 billion in 2022 and is predicted to increase to US$47.06 billion by 2030 [78]. As it is non-toxic, odorless, biodegradable, and easily absorbed by the body, its application is extensive in dairy products, mainly microencapsulated or coating films [79].
Alfaifi, Alkabli, and Elshaarawy [80] have developed a preservative using water-soluble chitosan (WSC) and 2-azido propanoic acid (APA). They have reported that the minimum inhibitory concentration (MIC) of WSC-APA completely suppresses the growth of E. coli O157:H7 and S. aureus in raw milk samples. The refrigerated milk samples were kept for 20 and 24 hours, respectively. Additionally, concentrations of 0.25 mg/mL of these associated compounds were sufficient to eliminate Staphylococcus spp after 6 days of storage. Moreover, there was a 99.7% reduction in coliforms after 10 days of storage.
Chitosan is a substance that can increase the shelf life of yogurts, improve their consistency, and reduce syneresis. According to a study by Zedan, Hosseini, and Mohammadi [81], adding high molecular weight chitosan (at concentrations of 2%, 4%, or 6%) to yogurt with Artemisia oil dracunculus can have positive effects. Samples containing 6% chitosan and 20% essential oils had a higher pH, lower acidity, and syneresis, as well as a proportional decrease in the count of total bacteria and yeasts as the chitosan concentration increased. However, in sensory evaluation, samples with only 2% chitosan received the highest overall score, indicating that higher concentrations may negatively affect consumer acceptance.
Fungi often contaminate cheese surfaces. However, it has been reported that chitosan incorporated into coating films can prevent such contamination. Dıblan et al. [82] conducted a study comparing different active films on the stability and microbial quality of kaşar cheese, concluding that chitosan films were more effective in controlling yeasts and fungi than films of potassium sorbate, nisin, and silver-substituted zeolite. In another study, Dong et al. [83] combined cell-free supernatant (CFS) of Lacticaseibacillus paracasei ALAC-4 with chitosan matrix in the active packaging of Mongolian cheese. This combination significantly inhibited molds and yeasts, particularly Candida albicans, for 15 days during storage at 4℃. The chitosan matrix also provided excellent mechanical properties.

5. Antimicrobials from Plant Sources

There is a growing interest in using plant-derived preservatives in the dairy sector, particularly those derived from vegetable by-products, herbs, and spices. This interest is fueled by the fact that certain plants that can thrive in harsh environments are capable of producing compounds with insecticidal, fungicidal, antibacterial, and antiviral properties [84]. These compounds are classified based on their main chemical groups, such as terpenes, phenylpropenes, terpenoids, allicin, and isothiocyanates [85], and they can interact with the bacterial cell membrane and disrupt its structure [86]. Due to their antimicrobial properties and high antioxidant capacity, these substances effectively preserve food [26]. The potential of plant-derived preservatives in the dairy industry is very promising and should inspire optimism for the future.

5.1. Plant Extracts

Plant extracts can be either liquid or solid. They can be extracted through various methods such as maceration, digestion, infusion, and decoction or emerging techniques such as Accelerated Solvent Extraction (ASE) and Ultrasound-Assisted Extraction [87]. The literature has widely reported the antimicrobial activity of these compounds in dairy products [19,20,21,22]. Moreira et al. [19] conducted a study to investigate the effects of decreasing the concentration of sodium chloride on the microbiological quality of fresh goat cheese. They achieved this by adding pequi extract (Caryocar brasiliense) to pasteurized milk (CM), cheese mass (CS), and under immersion (CIE). The results showed a significant decrease in LAB counts in CS and CIE samples after 21 days of storage. No activity of Enterobacteriaceae, Staphylococcus spp., E. coli, and bacteria from the coliform group was observed in any of the samples during storage [19].
In a study by Wanniatie et al. [20], the effect of adding red ginger extract to goat's milk yogurt was investigated. Results showed that the total bacteria count decreased significantly after adding 2%, but 4% extract increased the LAB count. In another study by Kamel et al. [21], carrot powder was incorporated into soft buffalo cheese with probiotics. The total bacterial count decreased from 7.5 to 7.3 log CFU/g in the product added with 0.6% of the compound. However, lactic acid bacteria and Bifidobacterium longum count increased at the end of 28 days in dosages of 0.4% and 0.6%. In terms of sensory evaluation, samples with 0.6% carrot powder obtained the lowest score for the flavor attribute on the first day of storage. However, no significant differences were observed in the flavor, texture, color, and appearance scores among all the samples at the end of the storage period.
Ávila Arribas et al. [22] also obtained satisfactory results when comparing the inhibitory effect of oregano plant extract (Origanum vulgare), savory (Satureja montana), hyssop (Hissopus officinalis) and tarragon (Artemisia dracunculus) under growth of different strains of Clostridium spp. in cheeses. The authors reported that all Clostridium strains were sensitive to EEs in at least one of the concentrations tested and that EEs from hyssop, lavender, and tarragon had lower MICs (<40 μL / mL). EEs from savory, lavender, and tarragon also delayed the appearance of vegetative cells of Clostridium spp. and delayed stewing in 2 weeks without harming the sensorial characteristics of the cheese.
Shehata et al. [88] conducted a study to evaluate the antimicrobial effect of taro leaf extract (TLE) at concentrations of 250 and 500 mg/L. The researchers found that this compound exhibited excellent activities against various microorganisms, including E. coli BA 12296, Salmonella Senftenberg ATCC 8400, Fusarium oxysporum ITEM 12591, and S. aureus, with inhibition zones of 19.3 ± 1.02 mm, 18.53 ± 0.75 mm, 12.93 ± 1.17 mm, and 11.33 ± 0.84 mm, respectively. Additionally, including the extract enhanced the antioxidant potential, the viability of L. paracasei, and the concentration of polyphenols in the drinks. These findings demonstrated good acceptability by consumers.
Plant extracts have become increasingly important in processing functional foods, and they are being used as alternatives to synthetic antimicrobials. Certain extracts' effectiveness against sporulating bacteria that cause late puffing in matured cheeses is a significant advantage. This defect is challenging to control and can result in significant losses for the food industry. Additionally, when the extracts are derived from industrial waste, they have greater environmental appeal and add value to the products.

5.2. Essential Oils

Essential oils (EO) are volatile hydrophobic liquids important for plants to defend themselves against bacteria, viruses, and fungi [89]. These compounds are highly effective in inhibiting Gram-negative bacteria, as their hydrophobic nature allows them to easily penetrate the lipopolysaccharide barrier of the outer membrane of these microorganisms. Once inside bacterial cells, they interfere with the transport mechanism of molecules, leading to cell death [90]. However, it is important to consider their minimum inhibitory concentrations (MIC) due to their pungent smell and potential toxicity at high concentrations [23].
Several EOs have already been studied in dairy products, such as thyme [91]; cinnamon [92]; oregano [93,94]; pink pepper [95]; lemon [96,97]; perilla leaf [98]; rosemary [99]; tangerine and orange [96]; mastic [100]; and, curry and cloves [101], demonstrating high preservative action, in addition to increasing shelf life and improving physicochemical and sensory characteristics. Jemaa et al. [91] compared the effect of thyme EO (Thymus capitatos) and its nanoemulsion on the quality of raw milk contaminated by S. aureus, demonstrating greater efficiency of the nanoemulsion in inhibiting the pathogen. However, milk with EO had greater antioxidant capacity and lower protein degradation. Furthermore, there were no statistical differences in the acidity content and peroxide inhibition in both treatments, demonstrating that thyme EO in solution or nanoencapsulated can improve milk quality and extend its shelf life.
In a study conducted by Abbes et al. [92], the antimicrobial efficacy of cinnamon essential oil (EO) in milk was examined. The results showed that Salmonella Hadar was completely inactivated at 3μg mL−1. Similarly, Hao et al. [94] investigated the antimicrobial properties of oregano EO, which is rich in carvacrol, against Escherichia coli and Staphylococcus aureus in milk. They used nuclear magnetic resonance (NMR) to study the mechanism of action and observed bacteriostatic and bactericidal effects, which were associated with changes in the cellular morphology of both microorganisms. In a previous study, Campos et al. [93] analyzed the inhibitory effect of oregano EO on filamentous fungi (Aspergillus flavus, Fusarium oxysporum, and Penicillium citrinum) as well as Escherichia coli and Staphylococcus aureus during the ripening of cheese. Oregano EO at a concentration of 0.02% w/w inhibited the growth of the tested strains over the 30-day maturation period. It reduced 7 logs (CFU/g) of S. aureus in the first hour of maturation and suppressed all viable cells of E. coli after three days of maturation. There were no changes to the pH and humidity of the cheese.
Listeria monocytogenes is a difficult pathogen to control in cheese production. In a recent study conducted by Dannenberg et al. [95], the efficacy of pink pepper essential oil (EO) as an antioxidant and antimicrobial agent was evaluated in Minas Frescal Cheese. The study also tested the oil against 18 bacteria in-vitro. The study showed a reduction of 1.3 log CFU/g of L. monocytogenes within 30 days, along with the inhibitory activity against seven spoilage bacteria, six pathogenic bacteria, and three bacteria with technological applications. In addition, there was a reduction of free radical DPPH. Another study by Fancello et al. [97] evaluated the effect of lemon EO (Citrus limon var pomia) on ricotta salata cheese stored at 5°C. The study showed that the EO had bactericidal effects on L. monocytogenes DSMZ and bacteriostatic effects on a mixture of L. monocytogenes strains. The chemical analysis of the liquid phase of this EO revealed that its main active compounds were linalyl acetate, limonene, and two isomers of citral.
Numerous studies have shown that essential oils (EOs) can enhance the quality of yogurts in terms of their microbiological, physical, chemical, and sensory characteristics [98,99,102,103]. For example, He et al. [98] investigated the impact of perilla leaf EO on the production of volatile compounds and the microbiological quality of yogurt. They found that adding 0.04% of this EO resulted in longer shelf life, the production of 69 volatile compounds (mainly limonenes), an increased concentration of terpenic substances, and greater sensory acceptance than the control yogurt. Similarly, Kamel et al. [99] examined the properties of rosemary EO as an alternative to synthetic preservatives in yogurt. They reported that rosemary EO exhibited antimicrobial activity against various microorganisms such as Escherichia coli, Staphylococcus aureus, Salmonella marcescens, total coliforms, yeasts (Candida albicans), and fungi (Aspergillus flavus). Moreover, adding rosemary EO enhanced the viability of LABs (lactic acid bacteria) and improved the overall acceptance of the yogurt.
Ice creams also represent a dairy matrix favorable to adding essential oils, as they are among the most consumed dairy products and are highly accepted among different age groups [104]. For example, EOs obtained from fruit residues from industrial processes are sustainable alternatives for incorporating natural antimicrobials into edible ice creams [96]. Tomar and Akarca [96] evaluated the microbiological, physical, chemical, and sensory properties of ice creams produced with EOs from lemon, tangerine, and orange peels in different concentrations (0.1%, 0.3%, and 0.5%). In conclusion, the authors did not identify the presence of total coliforms, Salmonella spp., Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes in any of the samples. Furthermore, the ice cream added with 0.5% EO and orange peel stood out with the lowest counts of aerobic mesophilic bacteria (3.80 log CFU/g), yeasts and fungi (3.71 log CFU/g), psychrophilic bacteria (4.01 log CFU/g) and Pseudomonas spp. (2.04 log CFU/g).
Mitropoulou et al. [100] researched ice cream to determine the minimum inhibitory concentration of microorganisms and the main active compounds of mastic EO (Pistacia lentiscus) by GC/MS. The study found that ice cream containing 0.2% (w/w) of EO gradually decreased the counts of Escherichia coli, Listeria monocytogenes, and Pseudomonas fragi during the first seven weeks of storage. On average, the count was reduced by 3 log CFU/g compared to control samples (p<0.05). The primary constituents of EO that contributed to this reduction were a-pinene (67.7%), myrcene (18.8%), and β-pinene (3.0%).
The use of EOs in dairy-based sweets has also been reported. Badola et al. [101] added EOs from curry leaves (0.05–0.15 ppm) and cloves (0.15–0.25 ppm) and evaluated the microbiological, antioxidant, physicochemical, and sensory characteristics. In addition to the greater antioxidant activity and greater presence of total phenolics, there was a reduction in the count of total bacteria, molds, and yeasts.
Although the numerous technological and antimicrobial properties of EOs are reported, their incorporation into the formulation of dairy products can also be limited by several factors [107]. For example, volatile organic compounds can quickly decompose in adverse environmental conditions, leading to loss of flavor, aroma, and antioxidant and antimicrobial efficacy [108]. Furthermore, antimicrobial phytochemicals in high concentrations can present toxicity and allergenic potential in sensitive individuals [109], in addition to possible interactions with food components, damaging their quality [110].
Although essential oils (EOs) have been shown to have antimicrobial properties, their use in certain dairy products should be limited. This is because they can negatively affect the product's sensory qualities [100,101]. In some cases, the undesirable flavor may be due to the presence of certain phytochemicals such as 3-carene, caryophyllene [105], and eugenol [106]. This issue is particularly noticeable in dairy products with high-fat content.

6. Encapsulation of Compounds with Antimicrobial Activity in Dairy Products

As previously mentioned, the effectiveness of antimicrobial compounds can be reduced by factors such as temperature, humidity, exposure to light, and undesirable interactions with dairy matrix constituents [70]. Encapsulation of these compounds is a viable technology for the protection and controlled release of natural antimicrobials during the production or storage of products [9]. This technique temporarily retains an active substance within a shell-forming material, releasing it under specific conditions such as water activity, time, temperature or pH [111]. In summary, encapsulation can increase the stability of natural antimicrobial compounds from different sources in dairy matrices, minimizing the risks of inactivation and loss of efficacy over time.
Several applications of natural antimicrobial compounds encapsulated in dairy products are described in the literature, including the use of polymeric nanocapsules [112], nanofibers [102], as well as agar microcapsules [113], soy protein isolates [114], and chitooligosaccharide [115]. The results are generally promising, mainly in increasing these products' quality, safety, and validity. However, selecting an appropriate encapsulation system must consider the interactions between the wall material with the antimicrobial compounds and the components of the food matrix to ensure better chemical stability of the substance and its delivery to the target [116]. More research is needed since most studies on dairy matrices are focused on milk [117,118], yogurts [102,115], and coating films for cheeses [48,119].

7. Conclusion

Dairy products are commonly exposed to microorganisms that can spoil the food and cause harm to human health. To counter this, researchers have investigated different sources and applications of natural antimicrobials that are effective against specific microorganisms found in dairy products. These antimicrobials can originate from microbes, animals, and plants. For instance, some natural antimicrobials such as lysozyme positively affect dairy products, particularly sensory and textural aspects. However, inconsistency exists in the literature regarding some factors, such as the ideal intrinsic conditions for the action of certain antimicrobials. Additionally, standardization is crucial to accurately interpret the results of studies, as the metabolism of the microorganism producing the antimicrobial can affect the results. Using natural antimicrobials in packaging and active films is a growing trend that can benefit dairy products during storage. However, some natural antimicrobials, such as some essential oils, can produce off-flavors in high-fat dairy products. Further studies are necessary to develop natural antimicrobials and validate their use in dairy products to gain a better understanding of their effects and interactions with the food matrix and active packaging and their impact on untested microorganisms.

Author Contributions

Conceptualization: Maria Eduarda Masques Soutelino, Adriana Cristina de Oliveira Silva, Ramon da Silva Rocha, Literature research: Maria Eduarda Masques Soutelino, Adriana Cristina de Oliveira Silva, Ramon da Silva Rocha, writing—original draft preparation: Maria Eduarda Masques Soutelino, Adrina Cristina Oliveira Silva; writing—review and editing: Adriana Cristina de Oliveira Silva; visualization: Maria Eduarda Masques Soutelino, Adriana Cristina de Oliveira Silva, Ramon da Silva Rocha; project administration: Ramon da Silva Rocha.

Data Availability Statement

Not applicable

Acknowledgments

Ramon S. Rocha wants to thank the grant 2022/14824-4, São Paulo Research Foundation (FAPESP), and Maria Eduarda M. Soutelino for Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for financial support.

Conflicts of Interest

The authors declare no conflict of interest.

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Table 1. Main studies on natural antimicrobials in dairy products.
Table 1. Main studies on natural antimicrobials in dairy products.
Natural antimicrobial Dairy product Dosage Main results Reference
Nisin Minas Frescal cheese 500 UI·mL Increased lag phase of S. aureus
Decrease in S. aureus counts in cheese dough and whey		 [34]
Nisin
 Dairy beverage
 3 MIC’s

 Associated with lactose laurate, it inhibited the growth of S. aureaus for 10 days, stabilizing the pH and relative viscosity. [36]
Natamicin Mozarella 0,25, 0,5 e 1 mg/dm 2 The dosage of 1mg/dm2 of natamycin associated with hydroxyethylcellulose film and pulverized reduced 5.28 Log CFU/g and 4.19 Log CFU/g (99.9%) of the fungal population of Penicillium spp.
Increased shelf life		 [46]
Natamicin Dairy beverage 1% pp/p Films incorporated with 1% natamycin and treated with UV rays for 6 minutes provided maximum anti-yeast activity against Rhodotorula mucilaginosa and Candida parapsilosis [53]
Reuterin (Lactobacillus reuteri) Fresh cheese 6 log10 cfu de Lb. reuteri The addition of Lb. reuteri significantly decreased (p>0,05) the Escherichia coli O157:H7 population in fresh cheeses after 28 days in 10% or 15% brine at 10°C or 25°C. [58]
Reuterin Yogurt 1,38mM e 6,9 mM Reuterin at a concentration of 1.38 mM showed a fungistatic effect and at a concentration of 6.9 mM it showed a fungicidal effect against a representative panel of contaminating fungi in dairy products. [54]
Lisozyme Milk 1.09mg/L Inhibited the growth of Bacillus megaterium, Bacillus mojavensis, Clavibacter michiganensis, Clostridium tyrobutyricum, Xanthomonas campestris and Escherichia coli, with an action similar to ampicillin and kanamycin, with the exception of Bacillus mojavensis, which showed resistance to lysozyme in milk samples subjected to heat treatment. [63]
Lysozyme hydrolyzed peptides Yogurt 0,4% Inhibition of molds and yeasts during 28 days of storage
Increased antioxidant capacity
Higher scores for color and appearance, taste and overall acceptance.		 [17]
Lactoferrin Milk 14,06 mg/mL and 112,5 mg/mL Significant decrease (p ≤ 0,05) in the count of Escherichia coli O157:H7 and Salmonella enterica at levels equal to or greater than 14,06 mg/mL and 112.5 mg/mL of lactoferrin, respectively. [72]
Lactoferrin Cheddar 5, 10, 15 and 20 mg/100g Dosages of 15 and 20 mg/100g resulted in a significant decrease in viable bacteria counts after 45 days.
22% decrease in total viable bacteria count and 72% increase in antioxidant capacity at a dosage of 20 mg/100g.
All dosages resulted in a significant increase in antioxidant capacity, but there was no change in the proximate and fatty acid composition, or in the color, flavor and texture scores of the cheeses.		 [73]
Conjugate of
chitosan and azidopropanoic acid		 Milk 0,25 mg/mL Total inhibition of the E. coli O157:H7 and S. aureus population in raw milk refrigerated at 4°C after 20 and 24 hours, respectively.
No species of S. aureaus were detected in milk after 6 days of storage.
99.7% reduction in coliform counts after 10 days of storage.		 [80]
Chitosan Kasar cheese 2% p/p Incorporated into the active film, it promoted total inhibition of yeasts and fungi in the cheese after 20 days of storage at 15 °C, in addition to a 1.5 log reduction in S.aureaus counts qnd increase in titratable acidity.
Pequi extract (Caryocar brasiliense) Caprin fresh cheese 6,25 mL/L Cheeses with extract added to the dough or immersed in the extract exhibited a significant decrease ( P < 0.05) in LAB counts after 21 days of storage.
Samples added from the extract showed lower luminosity (p<0.05)
The cheese added with pequi extract to the dough showed greater hardness (p<0.05)		 [19]
Red ginger extract Goat's milk yogurt 1%, 2%,3% e 4% p/p Decrease in total viable bacteria count after adding 2% of the extract
Addition of 2% extract reduced viscosity, density and LAB, but addition of 4% extract increased LAB count.		 [20]
Oregano OE Cured cheese 0,02% p/p Inhibition of the growth of Aspergillus flavus, Fusarium oxysporum and Penicillium citrinum, Escherichia coli and Staphylococcus aureus during 30 days of maturation
Reduction of seven logs in the S. aureus population in the first hour of ripening and inhibition of all viable E. coli cells after three days of ripening, without changes in the pH and humidity of the cheeses.		 [93]
Aroeira OE Ice cream 0,2% p/p Decrease of approximately 3 logcfu/g in the counts of E. coli, L. monocytogenes and P. fragi until the 7th week of storage.
Low sensory acceptance		 [100]
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