Version 1
: Received: 3 April 2024 / Approved: 3 April 2024 / Online: 4 April 2024 (09:18:09 CEST)
How to cite:
Sufar, E. K.; Hasanaliyeva, G.; Wang, J.; Bilsborrow, P.; Rempelos, L.; Volakakis, N.; Baranski, M.; Leifert, C. Effect of Rhizobium Seed Inoculation on Grain Legume Yield and Protein Content – a Systematic Review and Meta-Analysis. Preprints2024, 2024040344. https://doi.org/10.20944/preprints202404.0344.v1
Sufar, E. K.; Hasanaliyeva, G.; Wang, J.; Bilsborrow, P.; Rempelos, L.; Volakakis, N.; Baranski, M.; Leifert, C. Effect of Rhizobium Seed Inoculation on Grain Legume Yield and Protein Content – a Systematic Review and Meta-Analysis. Preprints 2024, 2024040344. https://doi.org/10.20944/preprints202404.0344.v1
Sufar, E. K.; Hasanaliyeva, G.; Wang, J.; Bilsborrow, P.; Rempelos, L.; Volakakis, N.; Baranski, M.; Leifert, C. Effect of Rhizobium Seed Inoculation on Grain Legume Yield and Protein Content – a Systematic Review and Meta-Analysis. Preprints2024, 2024040344. https://doi.org/10.20944/preprints202404.0344.v1
APA Style
Sufar, E. K., Hasanaliyeva, G., Wang, J., Bilsborrow, P., Rempelos, L., Volakakis, N., Baranski, M., & Leifert, C. (2024). Effect of Rhizobium Seed Inoculation on Grain Legume Yield and Protein Content – a Systematic Review and Meta-Analysis. Preprints. https://doi.org/10.20944/preprints202404.0344.v1
Chicago/Turabian Style
Sufar, E. K., Marcin Baranski and Carlo Leifert. 2024 "Effect of Rhizobium Seed Inoculation on Grain Legume Yield and Protein Content – a Systematic Review and Meta-Analysis" Preprints. https://doi.org/10.20944/preprints202404.0344.v1
Abstract
Variable and sometimes contrasting effects of using artificial rhizobium inocula to increase grain legume performance have been reported. The objectives of this systematic review and meta-analysis were therefore to (i) estimate the overall effect of rhizobium inocula on grain yield and protein content and (ii) identify potential confounding factors that explain the observed variation. A web of Science database literature search was carried out in 2016. Data from all field experiments, greenhouse and pot experiments that compared grain yields and protein contents in Rhizobium/Bradyrhizobium-inoculated with non-inoculated control crops were extracted and analyzed using previously described unweighted and weighted meta-analysis protocols. GRADE assessments of the strength of evidence of results from the weighted meta-analyses were carried which included an assessment of the risk of publication bias. Results: For unweighted meta-analyses we identified eligible data in 236 and 36 studies for grain yield and protein content respectively. For weighted meta-analyses we identified eligible data in 92 and 8 studies for grain yield and protein content respectively. Rhizobium inoculation was found to (i) significantly increase grain yield in both the unweighted (mean percent difference: 41%, 95%CI 36,46, p<0.0001) and weighted meta-analysis (Standardized mean difference: 1.56, 95%CI 1.26,1.87, p<0.0001) and (ii) significantly increase protein content in the unweighted meta-analysis (mean percent difference: 11%, 95%CI 5,18, p<0.0001), but not the weighed meta-analyses which only detected a trend (0.1>p>0.05) towards higher protein content in inoculated grain legume crops. Multi-level model-based weighted meta-analyses suggest that the (i) type of experiment, (ii) type of fertilizer used in experiments, (iii) grain legume type/species and (iv) the country in which experiments were carried out, were confounding factors. The main limitation of the evidence is that a large number of publications did not report measures of variation and could therefore not be included in the more scientifically sound weighted meta-analyses. However based on the large evidence-base available for grain yield it can be concluded that overall Bradyrhizobium/Rhizobium seed inoculation results can increase grain yield by ~40% compared with non-inoculated grain legume crops.
Biology and Life Sciences, Agricultural Science and Agronomy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.