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Submitted:
25 April 2024
Posted:
25 April 2024
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Run | Factors | RR120 Decolourization % |
Mean values |
S/N Ratio | ||||
---|---|---|---|---|---|---|---|---|
pH | Temp. | Carbon source |
Nitrogen Source |
Dye concentration |
||||
L1 | 4 | 25 | G | Y | 0.1 | 59.305 | 59.305 | -35.4618 |
L2 | 4 | 30 | F | P | 0.2 | 67.63 | 67.63 | -36.6028 |
L3 | 4 | 35 | S | AS | 0.3 | 64.04 | 64.04 | -36.129 |
L4 | 4 | 40 | L | AN | 0.4 | 66.62 | 66.62 | -36.4721 |
L5 | 4 | 45 | ST | U | 0.5 | 44.8 | 44.8 | -33.0256 |
L6 | 5 | 25 | F | AS | 0.4 | 62.74 | 62.74 | -35.9509 |
L7 | 5 | 30 | S | AN | 0.5 | 44.32 | 44.32 | -32.932 |
L8 | 5 | 35 | L | U | 0.1 | 59.335 | 59.335 | -35.4662 |
L9 | 5 | 40 | ST | Y | 0.2 | 65.825 | 65.825 | -36.3678 |
L10 | 5 | 45 | G | P | 0.3 | 64.91 | 64.91 | -36.2462 |
L11 | 6 | 25 | S | U | 0.2 | 70.515 | 70.515 | -36.9656 |
L12 | 6 | 30 | L | Y | 0.3 | 66.255 | 66.255 | -36.4244 |
L13 | 6 | 35 | ST | P | 0.4 | 57.39 | 57.39 | -35.1767 |
L14 | 6 | 40 | G | AS | 0.5 | 72.945 | 72.945 | -37.2599 |
L15 | 6 | 45 | F | AN | 0.1 | 65.86 | 65.86 | -36.3724 |
L16 | 7 | 25 | L | P | 0.5 | 69.87 | 69.87 | -36.8858 |
L17 | 7 | 30 | ST | AS | 0.1 | 84.27 | 84.27 | -38.5135 |
L18 | 7 | 35 | G | AN | 0.2 | 98.67 | 98.67 | -39.8837 |
L19 | 7 | 40 | F | U | 0.3 | 90.495 | 90.495 | -39.1325 |
L20 | 7 | 45 | S | Y | 0.4 | 76.91 | 76.91 | -37.7197 |
L21 | 8 | 25 | ST | AN | 0.3 | 89.72 | 89.72 | -39.0578 |
L22 | 8 | 30 | G | U | 0.4 | 85.945 | 85.945 | -38.6844 |
L23 | 8 | 35 | F | Y | 0.5 | 87.095 | 87.095 | -38.7999 |
L24 | 8 | 40 | S | P | 0.1 | 92.75 | 92.75 | -39.3463 |
L25 | 8 | 45 | L | AS | 0.2 | 94.135 | 94.135 | -39.475 |
Level | pH | Temperature | Carbon source | Nitrogen source | Dye concentration | |||||
---|---|---|---|---|---|---|---|---|---|---|
Mean | S/N | Mean | S/N | Mean | S/N | Mean | S/N | Mean | S/N | |
1 | 60.48 | -35.54 | 70.43 | -36.86 | 74.76 | -37.37 | 73.04 | -36.94 | 72.3 | -37.03 |
2 | 59.43 | -35.39 | 69.68 | -36.63 | 76.35 | -37.51 | 75.63 | -37.47 | 79.35 | -37.86 |
3 | 66.59 | -36.44 | 73.31 | -37.09 | 71.24 | -36.94 | 70.51 | -36.85 | 75.08 | -37.4 |
4 | 84.04 | -38.43 | 77.73 | -37.72 | 69.71 | -36.62 | 70.22 | -36.65 | 69.92 | -36.8 |
5 | 89.93 | -39.07 | 69.32 | -36.57 | 68.4 | -36.43 | 71.08 | -36.95 | 63.81 | -35.78 |
Delta | 30.5 | 3.68 | 8.4 | 1.15 | 7.95 | 1.08 | 5.41 | 0.81 | 15.55 | 2.08 |
Rank | 1 | 1 | 3 | 3 | 4 | 4 | 5 | 5 | 2 | 2 |
Bacterial | Code | Combination No. | Combination | Dye degradation% | |||||
Lysinibacilluscapsici PB300(T) | A | 2h | 4h | 6h | 8h | 10h | 12h | ||
1. | A+B | 30.25 | 45.47 | 74.32 | 80.16 | 99.59 | 96.76 | ||
2. | A+C | 39 | 51.37 | 69.85 | 87.54 | 89.68 | 99.44 | ||
3. | A+D | 47.17 | 65.87 | 78.82 | 79.21 | 89.27 | 99.81 | ||
Alcaligenesfaecalissubsp. Phenolicus DSM 16503(T) | B | 4. | B+C | 39.62 | 60.37 | 73.95 | 88.95 | 99.15 | 99.94 |
5. | B+D | 29.65 | 62.95 | 80.27 | 89.38 | 99.21 | 98.76 | ||
6. | C+D | 45.47 | 65.67 | 70.68 | 89.12 | 99.59 | 99.11 | ||
Acinetobacterbaumanni ATCC 19606(T) | C | 7. | A+B+C | 51.4 | 61.35 | 87.81 | 99.73 | 99.82 | 100 |
8. | A+B+D | 40.42 | 65.6 | 87.04 | 99.56 | 99.79 | 99.46 | ||
Pseudomonasaeruginosa JCM 5962(T) | D | 9. | A+C+D | 33.45 | 65 | 88.96 | 99.76 | 99.14 | 99.48 |
10. | A+B+C+D | 49.87 | 72.01 | 87.76 | 99.91 | 100 | 100 |
No. | Compound | Molecular formula | R.Time (min.) | Molecular Weight (g/mol) | Peak Area % | Peak Height % |
---|---|---|---|---|---|---|
1. | Butanoic acid | C5H10O2 | 3.433 | 102 | 49.38 | 23.88 |
2. | Pentanoic acid | C6H12O2 | 4.953 | 116 | 3.22 | 5.54 |
3. | Pentanoic acid | C6H12O2 | 5.345 | 116 | 0.90 | 2.85 |
4. | 2-Piperidinone | C5H9NO | 13.527 | 99 | 26.72 | 25.43 |
5. | Tridecanoic acid | C15H30O2 | 23.945 | 242 | 0.37 | 1.64 |
6. | 1,4-diazabicyclo[4.3.0]nonan-2,5-dione | C8H12N2O2 | 24.362 | 168 | 2.27 | 3.13 |
7. | Cyclo(L-prolyl-L-valine) | C10H16N2O2 | 25.498 | 196 | 8.77 | 19.45 |
8. | Pyrrolo[1,2-a]pyrazine-1,4-dione | C11H18N2O2 | 26.020 | 210 | 1.18 | 2.91 |
9. | L-Leucine | C25H47NO3 | 27.036 | 409 | 3.18 | 7.31 |
10. | Pyrrolo[1,2-a]pyrazine-1,4-dione | C11H18N2O2 | 27.356 | 210 | 4.01 | 7.81 |
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