Version 1
: Received: 30 April 2024 / Approved: 30 April 2024 / Online: 1 May 2024 (16:47:12 CEST)
Version 2
: Received: 28 May 2024 / Approved: 29 May 2024 / Online: 29 May 2024 (08:33:29 CEST)
How to cite:
Tanoeiro, J. R. D. A. G. P.; Fehrenbach, G. W.; Murray, P.; Pedrosa, R.; Chen, Y. Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production. Preprints2024, 2024050013. https://doi.org/10.20944/preprints202405.0013.v2
Tanoeiro, J. R. D. A. G. P.; Fehrenbach, G. W.; Murray, P.; Pedrosa, R.; Chen, Y. Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production. Preprints 2024, 2024050013. https://doi.org/10.20944/preprints202405.0013.v2
Tanoeiro, J. R. D. A. G. P.; Fehrenbach, G. W.; Murray, P.; Pedrosa, R.; Chen, Y. Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production. Preprints2024, 2024050013. https://doi.org/10.20944/preprints202405.0013.v2
APA Style
Tanoeiro, J. R. D. A. G. P., Fehrenbach, G. W., Murray, P., Pedrosa, R., & Chen, Y. (2024). Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production. Preprints. https://doi.org/10.20944/preprints202405.0013.v2
Chicago/Turabian Style
Tanoeiro, J. R. D. A. G. P., Rui Pedrosa and Yuanyuan Chen. 2024 "Evaluation of Dunaliella salina Growth in Different Salinities for Potential Application on Saline Wastewater Treatment and Biomass Production" Preprints. https://doi.org/10.20944/preprints202405.0013.v2
Abstract
This study investigated the adaptability of Dunaliella salina to different salinity levels, with emphasis on growth, pigment concentration, and desalination potential. It was found that among the 21 salinity levels, salinity 75 produced consistently favorable results in cell count (13.08 x 103 ± 1.41 x 103 cells/mL), dry biomass (2.46 ± 0.06 g/L), pigment content (chlorophyll a = 97.5 ± 0.1 µg/L, chlorophyll b = 123.6 ± 0.3 µg/L), and desalination (9.32 ± 0.47 reduction). Therefore, salinity 75 was selected for the final trial (scale-up), which revealed unanticipatedly high cell counts (58.96 x 103 ± 535.22 cells/mL), with dry biomass weight being statistically different (higher) than the expected (4.21 ± 0.02 g/L) (p<0.0001), most likely due to high cell count and energy reserve storage for high salinity adaption in the form of bio-compounds. Pigment growth continued (chlorophyll a = 95.4 ± 2.2 µg/L, chlorophyll b = 128.1 ± 5.1 µg/L), indicating pigment production under salt stress. Notably, desalination did not occur in this stage, possibly due to the necessity for a bigger initial inoculate, prolonged exposure or bioaccumulation becoming the prevailing mechanism over desalination. Nevertheless, the trial highlights D. salina's strong adaptation to various salinity levels. This suggests a promising future in halophyte research, particularly in understanding the mechanisms that prevent salt accumulation in cells and how to overcome this barrier. Additionally, these results suggest that microalgae could be a viable resource in saline-rich environments unsuitable for conventional agriculture, promoting industrial adaptation to adverse conditions.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
