Version 1
: Received: 10 May 2024 / Approved: 10 May 2024 / Online: 10 May 2024 (15:03:46 CEST)
How to cite:
Duan, X.; Wang, H. L.; Cao, Z. X.; Su, N.; Wang, Y. F.; Zheng, Y. Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster. Preprints2024, 2024050712. https://doi.org/10.20944/preprints202405.0712.v1
Duan, X.; Wang, H. L.; Cao, Z. X.; Su, N.; Wang, Y. F.; Zheng, Y. Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster. Preprints 2024, 2024050712. https://doi.org/10.20944/preprints202405.0712.v1
Duan, X.; Wang, H. L.; Cao, Z. X.; Su, N.; Wang, Y. F.; Zheng, Y. Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster. Preprints2024, 2024050712. https://doi.org/10.20944/preprints202405.0712.v1
APA Style
Duan, X., Wang, H. L., Cao, Z. X., Su, N., Wang, Y. F., & Zheng, Y. (2024). Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster. Preprints. https://doi.org/10.20944/preprints202405.0712.v1
Chicago/Turabian Style
Duan, X., Yu feng Wang and Ya Zheng. 2024 "Deficiency of ValRS-m Causes Male Infertility in Drosophila melanogaster" Preprints. https://doi.org/10.20944/preprints202405.0712.v1
Abstract
The Drosophila spermatogenesis has the renewal of germline stem cells, meiosis of spermatocytes, and morphological transformation of spermatids into mature sperm. We previously demonstrated that Ocnus (ocn) plays an essential role in spermatogenesis. ValRS-m (Valyl-tRNA synthetase, mito-chondrial) gene was downregulated in ocn RNAi testes. By hatchability testing, we found that ValRS-m-knockdown induced complete sterility of male flies. We also concluded that ValRS-m-knockdown inhibited the transition from spermatogonia to spermatocytes and caused accumulated spermatogonia in testes. To understand the intrinsic reason for this destruction, we further conducted transcriptome sequencing analysis in ValRS-m RNAi testes. A total of 7710 dif-ferentially expressed genes (DEGs) were identified to have at least a 2-fold change with a p-value of
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
