Version 1
: Received: 12 May 2024 / Approved: 12 May 2024 / Online: 13 May 2024 (08:20:29 CEST)
How to cite:
Huang, C.; Zhang, Y.; Yu, H.; Hou, C.; Guan, H.; Chen, X.; Xie, J. The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the cry1Ac Gene with Concomitant Enhancement of Its Insecticidal Activity. Preprints2024, 2024050787. https://doi.org/10.20944/preprints202405.0787.v1
Huang, C.; Zhang, Y.; Yu, H.; Hou, C.; Guan, H.; Chen, X.; Xie, J. The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the cry1Ac Gene with Concomitant Enhancement of Its Insecticidal Activity. Preprints 2024, 2024050787. https://doi.org/10.20944/preprints202405.0787.v1
Huang, C.; Zhang, Y.; Yu, H.; Hou, C.; Guan, H.; Chen, X.; Xie, J. The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the cry1Ac Gene with Concomitant Enhancement of Its Insecticidal Activity. Preprints2024, 2024050787. https://doi.org/10.20944/preprints202405.0787.v1
APA Style
Huang, C., Zhang, Y., Yu, H., Hou, C., Guan, H., Chen, X., & Xie, J. (2024). The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity. Preprints. https://doi.org/10.20944/preprints202405.0787.v1
Chicago/Turabian Style
Huang, C., Xiuping Chen and Jiajian Xie. 2024 "The Recombination of Insertion Sequence Results in Deletion of the 3′ End of the <em>cry1Ac</em> Gene with Concomitant Enhancement of Its Insecticidal Activity" Preprints. https://doi.org/10.20944/preprints202405.0787.v1
Abstract
The structure and expression of exogenous genes in transgenic crops are critical for the target traits. R7569 was a mutant event identified in this study with deletion at the 3' end of cry1Ac gene compared to the transgenic insect-resistant cotton MON531 event with commercial application. R7569 has the same exogenous insertion structure as MON531, but with a deletion in the 3' end of the cry1Ac gene and the terminator region. R7569 has a truncated cry1Ac gene with the length of 2,554 bp, encoding 881 amino acids. The transcription termination site was mainly concentrated downstream of the truncated position and extended 160-270 bp from the truncated position using rapid-amplification of cDNA ends (RACE). The transcript levels of cry1Ac genes of R7569 and MON531 decreased gradually at seedling, bud and bell stages, and the transcript levels of cry1Ac genes of R7569 were significantly higher than those of MON531 in seedling and bud stages, but there was no significant difference in the boll stage. The content of Cry1Ac protein in R7569 gradually decreased with the seedling, bud and boll stage, and the content of Cry1Ac protein in all three periods was higher than that of MON531. The insect resistance assay showed that the resistance levels of R7569 and MON531 were both at the high level, and the corrected mortality rate against bollworms was 99.5% and 95.2%, respectively, and there was no significant difference between them. The LC50 value of R7569 was 0.732ng/g dw, with a slope of 1.654 indicating a high level of resistance to bollworm. In this study, for the first time, we found a partial deletion of the target gene in commercially applied transgenic crops, and the partial deletion of the 3' end of the cry1Ac gene retained a better transcription, expression level and insecticidal activity, which can provide a specific case for the safety evaluation of transgenic crops.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
,
*
,
*
