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Standardizing Laser Speckle Orthogonal Contrast Imaging: Achieving Reproducible Measurements Across Instruments
Version 1
: Received: 29 May 2024 / Approved: 30 May 2024 / Online: 30 May 2024 (10:56:35 CEST)
A peer-reviewed article of this Preprint also exists.
Orlik, X.; Colin, E.; Plyer, A. Standardizing Laser Speckle Orthogonal Contrast Imaging: Achieving Reproducible Measurements across Instruments. Photonics 2024, 11, 585. Orlik, X.; Colin, E.; Plyer, A. Standardizing Laser Speckle Orthogonal Contrast Imaging: Achieving Reproducible Measurements across Instruments. Photonics 2024, 11, 585.
Abstract
Using two independent speckle imaging systems based on the recently published LSOCI method operating in contact mode on the skin, we assess the reproducibility of in vivo measurements and conduct an inter-instrument comparison. To this aim, we propose a calibration method to handle each imaging system as a comprehensive unit, which includes the laser source, optics, and camera. Our experimental results demonstrate that the microcirculation images produced by both instruments exhibit high accuracy and stability, with microcirculation activity values in excellent agreement, thereby paving the way for clinical applications.
Keywords
microcirculation; in vivo imaging; calibration; dynamic speckle; vascularization; LSOCI
Subject
Physical Sciences, Biophysics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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