Version 1
: Received: 6 June 2024 / Approved: 7 June 2024 / Online: 11 June 2024 (08:29:33 CEST)
How to cite:
Bryce-Sharron, N.; Nasiri, M.; Powell, T.; West, M. J.; Crickmore, N. A Shared Receptor Suggests a Common Ancestry Between an Insecticidal Bacillus thuringiensis Cry Protein and an Anti-cancer Parasporin. Preprints2024, 2024060520. https://doi.org/10.20944/preprints202406.0520.v1
Bryce-Sharron, N.; Nasiri, M.; Powell, T.; West, M. J.; Crickmore, N. A Shared Receptor Suggests a Common Ancestry Between an Insecticidal Bacillus thuringiensis Cry Protein and an Anti-cancer Parasporin. Preprints 2024, 2024060520. https://doi.org/10.20944/preprints202406.0520.v1
Bryce-Sharron, N.; Nasiri, M.; Powell, T.; West, M. J.; Crickmore, N. A Shared Receptor Suggests a Common Ancestry Between an Insecticidal Bacillus thuringiensis Cry Protein and an Anti-cancer Parasporin. Preprints2024, 2024060520. https://doi.org/10.20944/preprints202406.0520.v1
APA Style
Bryce-Sharron, N., Nasiri, M., Powell, T., West, M. J., & Crickmore, N. (2024). A Shared Receptor Suggests a Common Ancestry Between an Insecticidal <em>Bacillus thuringiensis</em> Cry Protein and an Anti-cancer Parasporin. Preprints. https://doi.org/10.20944/preprints202406.0520.v1
Chicago/Turabian Style
Bryce-Sharron, N., Michelle J West and Neil Crickmore. 2024 "A Shared Receptor Suggests a Common Ancestry Between an Insecticidal <em>Bacillus thuringiensis</em> Cry Protein and an Anti-cancer Parasporin" Preprints. https://doi.org/10.20944/preprints202406.0520.v1
Abstract
Cry toxins produced by the bacterium Bacillus thuringiensis are of significant agronomic value worldwide due to their potent and highly specific activity against various insect orders. However, some of these pore-forming toxins display specific activity against a range of human cancer cells whilst possessing no known insecticidal activity, Cry41Aa is one such toxin. Cry41Aa has similarities to its insecticidal counterparts in both its 3-domain toxic core structure and pore-forming abilities, but how it has evolved to target human cells is a mystery. This work shows that some insecticidal Cry toxins can enhance the toxicity of Cry41Aa against hepatocellular carcinoma cells, despite possessing no intrinsic toxicity themselves. This interesting crossover is not limited to human cancer cells, as Cry41Aa was found to inhibit some Aedes-active Cry toxins in mosquito larval assays. Here, we present findings that suggest that Cry41Aa shares a receptor with several insecticidal toxins indicating a stronger evolutionary relationship than their divergent activities might suggest.
Keywords
Cry41Aa; Cry1Ca; Aedes aegypti; HepG2
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
