Prerpints.org logo
Preprint
Short Note

Male Morphological Dimorphism in the Genji Firefly Nipponoluciola cruciata in Central Japan

Altmetrics

Downloads

74

Views

92

Comments

0

A peer-reviewed article of this preprint also exists.

This version is not peer-reviewed

Submitted:

16 June 2024

Posted:

17 June 2024

You are already at the latest version

Alerts
Abstract
The Japanese Genji firefly Nipponoluciola cruciata is famous for geographic variation in flash pattern. Such geographic variation has also been well studied in terms of ecology and molecular phylogeny. However, there are very few studies on geographic variation in morphology of this species. This study focused on morphological characteristics of males of this species in Yamanashi and Gunma Prefectures separated by the Kanto Mountains in central Japan. Previous studies have shown that this species in the two prefectures exhibits different genetic features as well as different flash patterns. This study aimed to explore morphological characteristics of males of this species and performed multivariate analysis using measurements previously published in the literature. As a result, allometric analysis between body length and elytra width suggested that this species exhibits different allometric patterns between the two prefectures. Moreover, cluster analysis of measurements of genitalia suggested that this species is completely separated into two different groups between the two prefectures. These results suggest the importance of further morphological studies to clarify the divergence of this species.
Keywords: 
Subject: Biology and Life Sciences  -   Insect Science

1. Introduction

The aquatic firefly Nipponoluciola cruciata (formerly named Luciola cruciata, Coleoptera, Lampyridae) is commonly called Genji-botaru and one of the most popular insects in Japan [1]. Interestingly, this species shows geographic variation in interflash intervals, which vary from about 2 s in western Japan to about 4 s in eastern Japan [2,3,4]. Molecular phylogenetic studies have also found genetic differentiation between the 4-s and 2-s flash types [5,6,7,8]. However, there are very few studies on geographic differences in morphological characteristics of this species. Ohba [9] examined several morphological measurements of this firefly such as body length, body width, pronotum length, and pronotum width throughout Japan. However, he found no significant geographic differences in mean values of measurements. He also showed geographic differences in regression of pronotum length on pronotum width but did not further multivariate analysis to compare regression lines. Imasaka [10,11] also examined several morphological measurements of this species throughout Japan but did not perform detailed statistical analysis.
Fortunately, the present author found that Imasaka [10,11] documented morphological measurements of this species in detail. Therefore, in this article, the author aims to reanalyze his data and statistically clarify characteristics of this species varying geographically.
This article focuses on morphological characteristics of this species in Gunma and Yamanashi Prefectures, central Japan (Figure 1). According to previous studies [4,12,13], this species in Gunma belongs to the 4-s flash type and in Yamanashi belongs to the 3-s flash type. In terms of geographic characteristics, the Kanto Mountains exist between the two prefectures. Previous studies [4,12,13,14] inferred that the divergence of N. cruciata in central Japan occurred between the 4-s flash type and the common ancestor of the 2-s and 3-s flash types about 11 Ma in the Miocene in relation to the uplifting of the Kanto Mountains. Therefore, the present author hypothesized that statistical differences would be also observed in the measurements of Imasaka [10,11] between the two prefectures.

2. Materials and Methods

The data used in this study were morphological measurements of male N. cruciata collected at two sites in Gunma Prefecture and two sites in Yamanashi Prefecture, central Japan as shown in Figure 1. They were obtained from Table 2 of Imasaka [10] and Table 2 of Imasaka [11].
The measurements used this study are as follows: body length, elytra width (width of the basal part), and lengths of six parts of genitalia. The statistical analysis was performed using two multivariate analysis methods: allometric analysis between body length and elytra width and cluster analysis of lengths of six parts of genitalia. The data of genitalia contain missing values. Specimens with missing values were excluded from cluster analysis. The specimen B82 shows an extremely small value of the part j of genitalia (Table 2 of Imasaka [11]). This was assumed to be a misprint and therefore this specimen was excluded from cluster analysis.
According to previous studies on the firefly Luciola parvula [15,16], allometric equations were applied to the data of body length and elytra width using standardized major axis regression with the smatr package in the R software [17]. The data were log10-transformed into the log-log relationship between body length (x) and elytra width (y). Consequently, the log10-transformed allometric equation was expressed as:
log10 y = log10 a + b log10 x
where a and b are constants. The constant b is the slope of the line, also known as the allometric coefficient.
The hierarchical cluster analysis of the lengths of six parts of genitalia was performed with the function hclust of the R software [17] using Ward’s method with Euclidean distance.

3. Results

As shown in Figure 2, the two allometric lines did not differ significantly in slope (χ2 = 2.07, df = 1, p = 0.150) but did differ significantly in elevation (y-intercept) (χ2 = 8.31, df = 1, p = 0.004). The common slope (b = 1.40) was significantly different from 1 (χ2 = 9.00, df = 2, p = 0.011), which indicated a positive allometry between body length and elytra width.
As shown in Figure 3, the results of cluster analysis showed that Gunma and Yamanashi specimens were completely classified into two major clusters.

4. Discussion

The results of allometric analysis showed that the allometry of N. cruciata in Gunma and Yamanashi Prefectures is represented by two parallel lines. These findings are similar to the allometric patterns of the Japanese terrestrial firefly Luciola parvula observed in Nagano Prefecture, central Japan [15]. However, as shown in Figure 2, there remains a possibility that N. cruciata in Gunma and Yamanashi Prefectures may show different allomeric slopes. Further studies are required with larger sample size to determine the allometric patterns of N. cruciata in central Japan.
The results of cluster analysis revealed that N. cruciata shows different shapes of male genitalia between Gunma and Yamanashi Prefectures. As mentioned above, this species in Gunma belongs to the 4-s flash type and in Yamanashi belongs to the 3-s flash type [4,12,13]. Therefore, this species of different flash types may exhibit different shapes of male genitalia in other areas. However, few studies have explored the relationship between morphology and flash typeｓ in this species. Ohba [18] mentioned that the 4-s type occasionally lacks a black marking in the pronotum but did not refer to differences in internal organs such as genitalia.
Ohba [9] suggested that variations in size of external structures in N. cruciata are larger within populations than among populations. However, the results of this study showed that multivariate analysis can be useful to detect size variations in both external and internal structures. The male morphological dimorphism of this species observed in this study may be associated with topography in this area. Further morphological studies are required to clarify the divergence of this species especially in mountainous areas of central Japan.

4. Conclusion

This article explored differences in morphological characteristics of the Japanese Genji firefly Nipponoluciola cruciata between Yamanashi and Gunma Prefectures separated by the Kanto Mountains in central Japan. Two multivariate analysis methods were performed using measurements previously published in the literature. First, allometric analysis between body length and elytra width suggested that this species exhibits different allometric patterns between the two prefectures. Second, cluster analysis of measurements of genitalia suggested that this species in the two prefectures is completely classified into different groups. These results suggest the importance of further morphological studies to clarify the divergence of this species.

Acknowledgments

The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author confirms sole responsibility for the following: study conception and design, data collection, analysis and interpretation of results, and manuscript preparation.

Conflicts of Interest

The author declares no conflicts of interest regarding the publication of this paper.

References

  1. Takada, K. (2011) Popularity of Different Lampyrid Species in Japanese Culture as Measured by Google Search Volume. Insects, 2, 336-342. [CrossRef]
  2. Kanda, S. (1935) Fireflies (Hotaru). Nippon Hakko Seibutsu Kenkyu Kai, Tokyo.
  3. Ohba, N. (1988) Genji Botaru (The Genji firefly). Bun-ichi Sogo Press, Tokyo.
  4. Iguchi, Y. (2010) Temperature-Dependent Geographic Variation in the Flashes of the Firefly Luciola cruciata (Coleoptera: Lampyridae). Journal of Natural History, 44, 861-867. [CrossRef]
  5. Suzuki, H., Sato, Y., Fujiyama, S. and Ohba, N. (1996) Allozymic Differentiation between Two Ecological Types of Flashing Behavior in the Japanese Firefly, Luciola cruciata. Japanese Journal of Entomology, 64, 682–691.
  6. Yoshikawa, T., Ide, K., Kubota, Y., Nakamura, Y., Takebe, H. and Kusaoke, H. (2001) Intraspecific Genetic Variation and Molecular Phylogeny of Luciola cruciata (Coleoptera: Lampyridae) Inferred from the Mitochondrial ND5 Gene Sequences. Japanese Journal of Entomology, 4, 117–127. [CrossRef]
  7. Suzuki, H., Sato, Y. and Ohba, N. (2002) Gene Diversity and Geographic Differentiation in Mitochondrial DNA of the Genji Firefly, Luciola cruciata (Coleoptera: Lampyridae). Molecular Phylogenetics and Evolution, 22, 193–205. [CrossRef]
  8. Kato, D., Suzuki, H., Tsuruta, A., Maeda, J., Hayashi, Y., Arima, K., Ito, Y., and Nagano, Y. (2020) Evaluation of the Population Structure and Phylogeography of the Japanese Genji Firefly, Luciola cruciata, at the Nuclear DNA Level Using RAD-Seq Analysis. Scientific Reports, 10, 1533. [CrossRef]
  9. Ohba, N. (2001) Geographical Variation, Morphology, and Flash Pattern of the Firefly, Luciola cruciata (Coleoptera: Lampyridae). Science Report of the Yokosuka City Museum, 48, 45–89.
  10. Imasaka, S. (2010) Geographic Variation in Luciola cruciata: 1st Report on Measurement Data from Central and Western Japan. Monthly Bulletin of Rikusei Hotaru Seitai Kenkyukai, 24, 1–18.
  11. Imasaka, S. (2012) Geographic Variation in Luciola cruciata: 2nd Report on Measurement Data from Central and Western Japan. Monthly Bulletin of Rikusei Hotaru Seitai Kenkyukai, 39, 1–16.
  12. Iguchi, Y. (2001) Pattern and Process of Geographical Diversification of Flashes in The Firefly Luciola cruciata in the Northern Part of Yamanashi Prefecture. Proceedings of the Japan Association for Fireflies Research (Zenkoku Hotaru Kenkyukai-shi), 34, 10–12. [CrossRef]
  13. Iguchi, Y. (2008) The Interflash Interval of the Genji-Firefly Luciola cruciata in the Central Part of Japan. Proceedings of the Japan Association for Fireflies Research (Zenkoku Hotaru Kenkyukai-shi), 41, 43–45. [CrossRef]
  14. Iguchi, Y. (2024) Katsuyama Famous for Its Dinosaurs and Fireflies in Relation to Alien Fireflies in Tatsuno and Kamikochi Tourism. Report of Laboratory of Biology, 11 May 2024. [CrossRef]
  15. Iguchi, Y. (2023) Allometric Approach to the Two Male Morphs in the Japanese Firefly Luci-ola parvula. Frontiers in Insect Science, 3. [CrossRef]
  16. Iguchi, Y. (2024) Geographic Differences in Allometric Patterns of Males of the Japanese Firefly Luciola parvula. Advances in Entomology, 12, 18-23. [CrossRef]
  17. R Core Team (2023) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna. Available at: https://www.r-project.org/.
  18. Ohba, N. (2004) Flash Communication Systems of Japanese Fireflies. Integrative and Comparative Biology, 44, 225-233. [CrossRef]
Preprints 109418 g001
Figure 1. Map of the study area in Gunma and Yamanashi Prefectures, central Japan. Numbers denote the following localities for specimens used in this study: 1. Tomioka, Gunma; 2. Yoshii, Gunma; 3. Takane, Yamanashi; 4. Tsuru, Yamanashi.
Figure 1. Map of the study area in Gunma and Yamanashi Prefectures, central Japan. Numbers denote the following localities for specimens used in this study: 1. Tomioka, Gunma; 2. Yoshii, Gunma; 3. Takane, Yamanashi; 4. Tsuru, Yamanashi.
Preprints 109418 g001
Preprints 109418 g002
Figure 2. Allometric relationship between body length and elytra width in N. cruciata in Gunma and Yamanashi Prefectures, central Japan. The standardized major axis regression lines were separately fitted to each prefecture.
Figure 2. Allometric relationship between body length and elytra width in N. cruciata in Gunma and Yamanashi Prefectures, central Japan. The standardized major axis regression lines were separately fitted to each prefecture.
Preprints 109418 g002
Preprints 109418 g003
Figure 3. Dendrogram of cluster analysis of measurements of genitalia in male N. cruciata in Gunma and Yamanashi Prefectures, central Japan. The specimens collected in Gunma and Yamanashi Prefectures are represented by Gu and Ya, respectively, in the x-axis label.
Figure 3. Dendrogram of cluster analysis of measurements of genitalia in male N. cruciata in Gunma and Yamanashi Prefectures, central Japan. The specimens collected in Gunma and Yamanashi Prefectures are represented by Gu and Ya, respectively, in the x-axis label.
Preprints 109418 g003
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.
Copyright: This open access article is published under a Creative Commons CC BY 4.0 license, which permit the free download, distribution, and reuse, provided that the author and preprint are cited in any reuse.
Prerpints.org logo

Preprints.org is a free preprint server supported by MDPI in Basel, Switzerland.

facebook logotwitter logolinkedin logo
MDPI Initiatives
Important Links
Subscribe

Disclaimer

Terms of Use

Privacy Policy

© 2024 MDPI (Basel, Switzerland) unless otherwise stated