Version 1
: Received: 19 June 2024 / Approved: 20 June 2024 / Online: 20 June 2024 (09:35:03 CEST)
How to cite:
Chevalier, M.; Ortis, M.; Olivieri, C.-V.; Vitale, S.; Paul, A.; Tonoyan, L.; Doglio, A.; Marsault, R. Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament.. Preprints2024, 2024061408. https://doi.org/10.20944/preprints202406.1408.v1
Chevalier, M.; Ortis, M.; Olivieri, C.-V.; Vitale, S.; Paul, A.; Tonoyan, L.; Doglio, A.; Marsault, R. Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament.. Preprints 2024, 2024061408. https://doi.org/10.20944/preprints202406.1408.v1
Chevalier, M.; Ortis, M.; Olivieri, C.-V.; Vitale, S.; Paul, A.; Tonoyan, L.; Doglio, A.; Marsault, R. Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament.. Preprints2024, 2024061408. https://doi.org/10.20944/preprints202406.1408.v1
APA Style
Chevalier, M., Ortis, M., Olivieri, C. V., Vitale, S., Paul, A., Tonoyan, L., Doglio, A., & Marsault, R. (2024). Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament.. Preprints. https://doi.org/10.20944/preprints202406.1408.v1
Chicago/Turabian Style
Chevalier, M., Alain Doglio and Robert Marsault. 2024 "Herpes Simplex Virus Type 1 Infection of Human Periodontal Ligament." Preprints. https://doi.org/10.20944/preprints202406.1408.v1
Abstract
The periodontal ligament (PDL) is a complex connective tissue that connects the tooth root to the dental alveolar bone and plays crucial mechanical roles. PDL also exhibits regenerative roles and regulatory functions to maintain per-iodontium integrity and homeostasis. While PDL exposure to oral microbial pathogens is common, virtually nothing is known regarding viral infections of PDL. In particular, human herpes simplex virus type 1 (HSV-1) persistently infects the oral cavity through infections of the oral epithelium, connective tis-sue and neurons. While the oral spread of HSV-1 is generally asymptomatic, this virus has also been implicated in various oral pathologies. In this study, using a primary cell model derived from PDL (PDL-cells), and whole surgical fragments of PDL, we provide evidence supporting the efficient infection of PDL by HSV-1 and the promotions of cytopathic effects. Infection of PDL by HSV-1 was also associated with an acute innate inflammatory response, as il-lustrated by the production of the antiviral interferons and pro-inflammatory cytokines. Furthermore, this inflammatory response to HSV-1 was exacerbated in the presence of bacterial-derived products, such as peptidoglycans. This work therefore highlights the ability of HSV-1 to infect mesenchymal cells from PDL, suggesting that PDL may serve as a viral reservoir for the perio-dontal spread of HSV-1. Moreover, this raises questions about HSV-1 oral pathogenesis, as HSV-1-associated cytopathic and inflammatory effects may contribute to profound alterations of PDL integrity and functioning.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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